Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	851,798	A→G	L267P (CTT→CCT)	opgE ←	OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	851,798	0	A	G	100.0%	250.8 / NA	67	L267P (CTT→CCT)	opgE	OPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/35);  total (32/35)

ATGCTGGCGATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGG  >  NC_000913/851654‑851945                                                                                                                                                 |                                                                                                                                                    aTGCTGGCGATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGAtt                                                                                                                                               >  1:1144991/1‑151 (MQ=255)    cTGGCTATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCt                                                                                                                                            >  1:422261/1‑151 (MQ=255)      ggCGATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTg                                                                                                                                           <  1:778382/150‑1 (MQ=255)      ggCGATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGc                                                                                                                                          <  2:1038172/151‑1 (MQ=255)       gCGATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCg                                                                                                                                         <  1:361821/151‑1 (MQ=255)        cGATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGt                                                                                                                                        >  2:425822/1‑151 (MQ=255)         gATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGCCAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTg                                                                                                                                       >  1:320867/1‑151 (MQ=255)          aTAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCTCGCGATTTGCCAGGGTGACAAAGTTAGCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGGCAGGCGATTTCTGCGTgg                                                                                                                                      <  1:652760/151‑1 (MQ=255)            aGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTgg                                                                                                                                      >  1:1145485/1‑149 (MQ=255)            aGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAg                                                                                                                                    <  1:989176/151‑1 (MQ=255)            aGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAg                                                                                                                                    <  1:587630/151‑1 (MQ=255)               ggTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTTCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCa                                                                                                                                 <  1:432572/151‑1 (MQ=255)                 tATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACt                                                                                                                               >  2:767050/1‑151 (MQ=255)                    cGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTgg                                                                                                                             <  1:901886/150‑1 (MQ=255)                        ttCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTggcggc                                                                                                                         >  2:1084312/1‑150 (MQ=255)                         tCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCa                                                                                                                        >  2:605571/1‑150 (MQ=255)                           gCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATg                                                                                                                     <  2:649498/151‑1 (MQ=255)                            ccGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGt                                                                                                                    >  2:677897/1‑151 (MQ=255)                            ccGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGt                                                                                                                    >  1:52141/1‑151 (MQ=255)                               aTTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGt                                                                                                                 <  2:887727/151‑1 (MQ=255)                               aTTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGt                                                                                                                 <  1:721805/151‑1 (MQ=255)                                 ttGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTc                                                                                                                >  2:877284/1‑150 (MQ=255)                                  tGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTc                                                                                                                >  2:270046/1‑149 (MQ=255)                                    aCCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCa                                                                                                            <  2:1167374/151‑1 (MQ=255)                                    aCCCTGGTTGGAAAACCACCAGGTATGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCTATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGTCACTGTCGGCAATGTAGTCAg                                                                                                              <  1:712487/149‑1 (MQ=255)                                      ccTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCaaa                                                                                                          <  2:677544/151‑1 (MQ=255)                                         ggTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCTCTGGCGGCAATGTAGTCAGCAAatat                                                                                                       <  1:979870/151‑1 (MQ=255)                                         ggTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAata                                                                                                        <  2:1107020/150‑1 (MQ=255)                                         ggTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAata                                                                                                        <  1:132231/150‑1 (MQ=255)                                         ggTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAat                                                                                                         <  1:1025712/149‑1 (MQ=255)                                           ttGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATc                                                                                                      >  2:955308/1‑150 (MQ=255)                                                   ccaccaGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTa                                                                                             <  2:990717/151‑1 (MQ=255)                                                        aGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACgct                                                                                        <  2:816455/151‑1 (MQ=255)                                                             tGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTgg                                                                                   >  1:415608/1‑151 (MQ=255)                                                              ggAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACgctgct                                                                                     <  1:382115/148‑1 (MQ=255)                                                                  gCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAAc                                                                              <  2:777085/151‑1 (MQ=255)                                                                  gCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAAc                                                                              >  1:978657/1‑151 (MQ=255)                                                                    ccGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACgg                                                                            <  2:839421/151‑1 (MQ=255)                                                                     cgcgcgATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACggg                                                                           <  2:1160492/151‑1 (MQ=255)                                                                          gATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTAtt                                                                      <  2:961356/151‑1 (MQ=255)                                                                           aTTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTg                                                                     >  2:915130/1‑151 (MQ=255)                                                                              tGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTc                                                                   <  2:1090119/150‑1 (MQ=255)                                                                                 cAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAg                                                               <  2:529768/151‑1 (MQ=255)                                                                                  aGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAg                                                               >  1:978520/1‑150 (MQ=255)                                                                                  aGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGt                                                              >  1:45904/1‑151 (MQ=255)                                                                                   gggTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTGTTGTCCCAGTg                                                             >  2:722436/1‑151 (MQ=255)                                                                                    ggTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTg                                                             >  1:190163/1‑150 (MQ=255)                                                                                    ggTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTg                                                             >  1:358041/1‑150 (MQ=255)                                                                                         cAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGcc                                                       >  1:1135643/1‑151 (MQ=255)                                                                                             gTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCaa                                                     >  1:490252/1‑149 (MQ=255)                                                                                               tATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAgg                                                  <  1:376957/150‑1 (MQ=255)                                                                                               tATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAgg                                                  <  1:316985/150‑1 (MQ=255)                                                                                                  ccTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCAcc                                              <  1:766877/151‑1 (MQ=255)                                                                                                  ccTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCAcc                                              >  2:84206/1‑151 (MQ=255)                                                                                                   cTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCg                                             >  2:973548/1‑151 (MQ=255)                                                                                                     gAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAg                                           <  2:1114449/151‑1 (MQ=255)                                                                                                      aaaCTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAgg                                          >  2:1093343/1‑151 (MQ=255)                                                                                                         cTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGc                                        >  2:345790/1‑150 (MQ=255)                                                                                                         cTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGc                                        >  2:964153/1‑150 (MQ=255)                                                                                                           gtgGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCa                                       <  2:1094052/149‑1 (MQ=255)                                                                                                             ggTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATcgc                                   >  2:1181855/1‑151 (MQ=255)                                                                                                                          cAACGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTc                      <  2:1156198/151‑1 (MQ=255)                                                                                                                            aCGCGATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCAc                    >  1:656133/1‑151 (MQ=255)                                                                                                                               cgATTGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCg                  >  1:837653/1‑150 (MQ=255)                                                                                                                                   tGAGCGTTAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATca              <  1:104537/150‑1 (MQ=255)                                                                                                                                          tAAGCCAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATcaccaccac       <  2:52156/150‑1 (MQ=255)                                                                                                                                              ccAGGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAgg  >  1:935871/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    ATGCTGGCGATAGCGGTATCGTATTCGCCGATTTGACCCTGGTTGGAAAACCACCAGGTCTGGAAGCCCGCGCGATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGG  >  NC_000913/851654‑851945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑