Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,259,406	G→A	G466G (GGC→GGT)	dauA ←	C4‑dicarboxylic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,259,406	0	G	A	100.0%	296.1 / NA	75	G466G (GGC→GGT)	dauA	C4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/30);  total (45/30)

CAGACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGGCCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTC  >  NC_000913/1259259‑1259553                                                                                                                                                    |                                                                                                                                                    cAGACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGAcc                                                                                                                                                   >  2:228959/1‑150 (MQ=255)   gACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAAt                                                                                                                                                <  1:667131/151‑1 (MQ=255)     cGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATaa                                                                                                                                              <  1:707778/151‑1 (MQ=255)         tCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAAcgc                                                                                                                                           <  1:212516/150‑1 (MQ=255)             aaaaCGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACgcgc                                                                                                                                         >  2:89107/1‑148 (MQ=255)                aCGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAccc                                                                                                                                   <  1:933816/151‑1 (MQ=255)                 cGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCa                                                                                                                                  <  1:205457/151‑1 (MQ=255)                  gCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAAcc                                                                                                                                   >  2:903933/1‑149 (MQ=255)                       aaCGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGCATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACa                                                                                                                             >  1:372494/1‑150 (MQ=255)                         cGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCg                                                                                                                          <  1:1115014/151‑1 (MQ=255)                          gCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGt                                                                                                                         >  2:480551/1‑151 (MQ=255)                           cATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTc                                                                                                                        <  1:1061770/151‑1 (MQ=255)                           cATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTc                                                                                                                        <  1:468609/151‑1 (MQ=255)                           cATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTc                                                                                                                        >  2:523092/1‑151 (MQ=255)                           cATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTc                                                                                                                        <  1:882022/151‑1 (MQ=255)                             tCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTg                                                                                                                      >  2:660639/1‑151 (MQ=255)                                  accaccAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAAc                                                                                                                  >  1:675787/1‑150 (MQ=255)                                   ccaccaGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACAt                                                                                                                >  1:471918/1‑151 (MQ=255)                                       caGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTAc                                                                                                            <  1:334702/151‑1 (MQ=255)                                        aGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACg                                                                                                           <  1:873754/151‑1 (MQ=255)                                           aTCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACt                                                                                                        >  2:1157130/1‑151 (MQ=255)                                             cAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTa                                                                                                       <  2:818012/150‑1 (MQ=255)                                             cAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTa                                                                                                       <  2:391909/150‑1 (MQ=255)                                             cAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTAc                                                                                                      >  1:144593/1‑151 (MQ=255)                                             cAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTAc                                                                                                      <  1:417152/151‑1 (MQ=255)                                               aGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCg                                                                                                    >  1:128937/1‑151 (MQ=255)                                               aGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCg                                                                                                    <  1:804528/151‑1 (MQ=255)                                                gTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCgg                                                                                                   <  1:652884/151‑1 (MQ=255)                                                   ccGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGc                                                                                                <  1:1123386/151‑1 (MQ=255)                                                   ccGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGc                                                                                                >  2:353271/1‑151 (MQ=255)                                                       aaCGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAgg                                                                                            >  2:149015/1‑151 (MQ=255)                                                       aaCGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAgg                                                                                            >  2:736998/1‑151 (MQ=255)                                                        aCGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGc                                                                                           <  1:980444/151‑1 (MQ=255)                                                        aCGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGc                                                                                           >  1:583566/1‑151 (MQ=255)                                                          ggCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGa                                                                                         <  1:942500/151‑1 (MQ=255)                                                            cATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGt                                                                                       >  2:994708/1‑151 (MQ=255)                                                                ccACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCAt                                                                                    >  2:138469/1‑150 (MQ=255)                                                                      cAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCg                                                                             >  1:901819/1‑151 (MQ=255)                                                                       aGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCg                                                                             >  1:54456/1‑150 (MQ=255)                                                                           tCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATacg                                                                        >  2:1095437/1‑151 (MQ=255)                                                                            cacaATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATacg                                                                        >  2:1175621/1‑150 (MQ=255)                                                                                aTCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGc                                                                    >  2:1083297/1‑150 (MQ=255)                                                                                     tttGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAAc                                                              >  1:578947/1‑151 (MQ=255)                                                                                             cAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGa                                                      <  1:176304/151‑1 (MQ=255)                                                                                              aaGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGAt                                                     >  2:113839/1‑151 (MQ=255)                                                                                              aaGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGAt                                                     >  1:614691/1‑151 (MQ=255)                                                                                                gACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGc                                                   >  2:944872/1‑151 (MQ=255)                                                                                                  cGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCa                                                 <  2:591239/151‑1 (MQ=255)                                                                                                   gTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAg                                                <  1:979250/151‑1 (MQ=255)                                                                                                    tGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAg                                                >  1:627693/1‑150 (MQ=255)                                                                                                     gACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGc                                               >  2:744737/1‑150 (MQ=255)                                                                                                       cTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCAc                                             >  2:618811/1‑150 (MQ=255)                                                                                                           aGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATcc                                        >  1:832307/1‑151 (MQ=255)                                                                                                             gTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATcccc                                      <  1:899991/151‑1 (MQ=255)                                                                                                               ccGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCa                                     <  1:89083/150‑1 (MQ=255)                                                                                                                 gTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGc                                  >  1:987994/1‑151 (MQ=255)                                                                                                                 gTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGc                                  <  2:1039025/151‑1 (MQ=255)                                                                                                                  tGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCt                                 >  1:830893/1‑151 (MQ=255)                                                                                                                    aaTAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGAGCCCCACGc                                  >  1:245768/1‑148 (MQ=255)                                                                                                                     aTAAGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGAt                              <  1:228912/151‑1 (MQ=255)                                                                                                                        aGCCTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGc                           >  2:809742/1‑151 (MQ=255)                                                                                                                          ccTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCaa                         <  1:522959/151‑1 (MQ=255)                                                                                                                           cTTCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCaa                         >  1:583593/1‑150 (MQ=255)                                                                                                                             tCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATa                       <  2:690267/150‑1 (MQ=255)                                                                                                                             tCAGCAGCAGCAAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATa                       >  2:156509/1‑150 (MQ=255)                                                                                                                                   gcagcaAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCatat                >  2:281016/1‑151 (MQ=255)                                                                                                                                       caAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCaaa            <  1:965191/151‑1 (MQ=255)                                                                                                                                       caAAAAACAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCaa             >  2:750590/1‑150 (MQ=255)                                                                                                                                        aaaaaaCAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCaaa            >  1:419770/1‑150 (MQ=255)                                                                                                                                         aaaaaCAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAaac           >  1:263555/1‑150 (MQ=255)                                                                                                                                            aaCAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAaacaac        >  2:1022798/1‑150 (MQ=255)                                                                                                                                             aCAGCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAacac      >  2:649194/1‑151 (MQ=255)                                                                                                                                                gCGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGt   >  2:505468/1‑151 (MQ=255)                                                                                                                                                 cGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTc  <  2:583708/151‑1 (MQ=255)                                                                                                                                                 cGGACCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTc  >  2:243920/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGACGCTTCACAAAACGCTGGAACGCATCAAGACCACCAGCATCAAGTACCGGAACGGCATCCCACTTCAGAATCACAATCCGTTTGCCTTCAAGACGTGACTCCAGGTCCGTGAATAAGCCTTCAGCAGCAGCAAAAAACAGCGGGCCAATAACGCGCAGAACCAGGACATCGTCTGGAACATCTACGACTACCGGTGCCAGGCGAGTCATACGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTC  >  NC_000913/1259259‑1259553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑