Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,325,514	T→C	F257L (TTC→CTC)	yciQ →	enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,325,514	0	T	C	100.0%	386.0 / NA	100	F257L (TTC→CTC)	yciQ	enhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/55);  total (45/55)

CGATCCGTTTTATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGTTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTT  >  NC_000913/1325366‑1325659                                                                                                                                                     |                                                                                                                                                  cgATCCGTTTTATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTc                                                                                                                                                 >  2:856635/1‑151 (MQ=255)  gATCCGTTTTATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTcc                                                                                                                                                >  1:710891/1‑151 (MQ=255)  gATCCGTTTTATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTcc                                                                                                                                                >  1:600835/1‑151 (MQ=255)       gTTTTATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGtt                                                                                                                                           >  2:73890/1‑151 (MQ=255)         tttATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTtg                                                                                                                                         >  1:675669/1‑151 (MQ=255)         tttATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTtg                                                                                                                                         >  1:390946/1‑151 (MQ=255)            aTCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTtgtg                                                                                                                                       <  2:631545/150‑1 (MQ=255)            aTCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTgg                                                                                                                                      <  2:172343/151‑1 (MQ=255)              cGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTgg                                                                                                                                      >  2:441029/1‑149 (MQ=255)              cGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGAt                                                                                                                                    <  2:984739/151‑1 (MQ=255)               gTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTTTGGATg                                                                                                                                   <  1:953482/151‑1 (MQ=255)                    gATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTtat                                                                                                                              <  1:948239/151‑1 (MQ=255)                       ttCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTatat                                                                                                                            >  1:279928/1‑150 (MQ=255)                       ttCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTatat                                                                                                                            <  1:146164/150‑1 (MQ=255)                          aCGGTCCTCTACCGGTGGCCTCACGCTTTACTTAGCAATGCTTCGGCTCCTCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATctct                                                                                                                        >  1:131168/1‑151 (MQ=255)                          aCGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATctct                                                                                                                        >  1:252853/1‑151 (MQ=255)                           cGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTg                                                                                                                       >  2:276961/1‑151 (MQ=255)                           cGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTg                                                                                                                       <  1:16963/151‑1 (MQ=255)                            ggTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTgg                                                                                                                      <  2:783875/151‑1 (MQ=255)                            ggTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTgg                                                                                                                      >  2:1004762/1‑151 (MQ=255)                             gTCCTCTACCGCTGGCCGCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTgg                                                                                                                      >  1:268364/1‑150 (MQ=255)                              tCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGa                                                                                                                     >  1:494744/1‑150 (MQ=255)                               ccTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGaa                                                                                                                    <  1:34142/150‑1 (MQ=255)                               ccTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGa                                                                                                                     >  1:792762/1‑149 (MQ=255)                                  ctACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAgc                                                                                                                  <  2:778002/149‑1 (MQ=255)                                   tACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAgcgc                                                                                                                >  2:1161963/1‑150 (MQ=255)                                    aCCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAgcgc                                                                                                                >  2:358528/1‑149 (MQ=255)                                    aCCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAgcgc                                                                                                                <  1:1113580/149‑1 (MQ=255)                                    aCCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCa                                                                                                               <  1:971231/150‑1 (MQ=255)                                                 cGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTAc                                                                                                  <  1:616023/150‑1 (MQ=255)                                                 cGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACg                                                                                                 <  1:1169927/151‑1 (MQ=255)                                                   cTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGcc                                                                                               >  2:841432/1‑151 (MQ=255)                                                    tttACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCg                                                                                              >  1:413566/1‑151 (MQ=255)                                                        cTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGt                                                                                            >  1:495202/1‑149 (MQ=255)                                                          tAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAg                                                                                          <  2:880726/149‑1 (MQ=255)                                                           aGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGAt                                                                                        <  2:24639/150‑1 (MQ=255)                                                            gCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGAtgt                                                                                      >  2:1022808/1‑151 (MQ=255)                                                              aaTGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGAtgtg                                                                                     >  1:22606/1‑150 (MQ=255)                                                              aaTGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGa                                                                                    <  2:1042739/151‑1 (MQ=255)                                                                tGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGACCGCAATTTACGCCGGTAGATGTGAtt                                                                                  >  1:725051/1‑151 (MQ=255)                                                                 gCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGAtt                                                                                  <  2:144894/150‑1 (MQ=255)                                                                 gCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGAtt                                                                                  <  1:1178405/150‑1 (MQ=255)                                                                   cgcGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTGCCCTCCGCGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGaa                                                                               <  1:576823/149‑1 (MQ=255)                                                                   cTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGa                                                                                >  1:408394/1‑150 (MQ=255)                                                                    tCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGaa                                                                               >  2:1182735/1‑150 (MQ=255)                                                                     cGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGaa                                                                               <  1:856437/149‑1 (MQ=255)                                                                        cTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCg                                                                           >  2:694158/1‑150 (MQ=255)                                                                          ccGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGAt                                                                         <  1:133405/150‑1 (MQ=255)                                                                           cGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGt                                                                       >  2:347070/1‑151 (MQ=255)                                                                            gCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGt                                                                       <  2:959251/150‑1 (MQ=255)                                                                            gCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTc                                                                      >  2:1146459/1‑151 (MQ=255)                                                                             cAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCa                                                                     <  1:987001/151‑1 (MQ=255)                                                                             cAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCa                                                                     <  1:333931/151‑1 (MQ=255)                                                                              aaaCGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCAt                                                                    <  1:932221/151‑1 (MQ=255)                                                                               aaCGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCAtt                                                                   >  1:271887/1‑151 (MQ=255)                                                                                acgacgAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTc                                                                  <  2:885213/151‑1 (MQ=255)                                                                                  gacgaATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCg                                                                <  1:587910/151‑1 (MQ=255)                                                                                     gaATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGcc                                                              >  2:524308/1‑150 (MQ=255)                                                                                     gaATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGcc                                                              >  2:697497/1‑150 (MQ=255)                                                                                      aaTATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGcc                                                              >  2:459378/1‑149 (MQ=255)                                                                                         atTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGAtt                                                         >  2:7101/1‑151 (MQ=255)                                                                                           tttCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTac                                                       >  1:810371/1‑151 (MQ=255)                                                                                           tttCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTac                                                       >  2:392691/1‑151 (MQ=255)                                                                                           tttCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTa                                                        <  1:146516/150‑1 (MQ=255)                                                                                           tttCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTa                                                        <  1:275195/150‑1 (MQ=255)                                                                                           tttCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTa                                                        <  1:977217/150‑1 (MQ=255)                                                                                            ttCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTac                                                       <  1:842104/150‑1 (MQ=255)                                                                                             tCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTaca                                                      <  2:1065872/150‑1 (MQ=255)                                                                                              cAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTacac                                                     <  1:163199/150‑1 (MQ=255)                                                                                               aGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGc                                                   <  1:524174/151‑1 (MQ=255)                                                                                                 ccATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGccc                                                 <  2:972522/151‑1 (MQ=255)                                                                                                       tcttTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCgg                                               <  2:711057/147‑1 (MQ=255)                                                                                                           ttACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTa                                        <  2:600978/150‑1 (MQ=255)                                                                                                            tACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTAc                                       <  2:521431/150‑1 (MQ=255)                                                                                                            tACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACg                                      >  1:227154/1‑151 (MQ=255)                                                                                                               ccTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGtct                                   <  2:894029/151‑1 (MQ=255)                                                                                                                cTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGtctc                                  >  2:1183352/1‑151 (MQ=255)                                                                                                                   cACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGAt                               <  2:621037/151‑1 (MQ=255)                                                                                                                   cACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGAt                               >  2:1087817/1‑151 (MQ=255)                                                                                                                    aCGTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATg                              <  1:908152/151‑1 (MQ=255)                                                                                                                      gTCATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCg                            <  2:52615/151‑1 (MQ=255)                                                                                                                        cATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGa                           >  1:1042462/1‑150 (MQ=255)                                                                                                                        cATCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGa                           >  1:58375/1‑150 (MQ=255)                                                                                                                         aTCGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGa                           >  1:1022708/1‑149 (MQ=255)                                                                                                                           cGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGc                        <  2:675827/150‑1 (MQ=255)                                                                                                                           cGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGc                        <  1:137399/150‑1 (MQ=255)                                                                                                                           cGTTGTTAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCt                       >  1:628892/1‑151 (MQ=255)                                                                                                                            gttgttAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTa                      <  1:1022569/151‑1 (MQ=255)                                                                                                                              tgttAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGt                    >  1:463467/1‑151 (MQ=255)                                                                                                                               gttAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGt                    <  1:276893/150‑1 (MQ=255)                                                                                                                                ttAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGttt                  >  1:564534/1‑151 (MQ=255)                                                                                                                                ttAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGtt                   <  2:252911/150‑1 (MQ=255)                                                                                                                                 tAATTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGttt                  <  2:710601/150‑1 (MQ=255)                                                                                                                                  aaTTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTac                >  1:1027862/1‑151 (MQ=255)                                                                                                                                  aaTTTGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTa                 <  2:476512/150‑1 (MQ=255)                                                                                                                                      tGGTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTacgac             <  1:693955/150‑1 (MQ=255)                                                                                                                                        gTTTCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGAt          <  2:808430/151‑1 (MQ=255)                                                                                                                                          ttCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATa         <  1:1004528/150‑1 (MQ=255)                                                                                                                                          ttCCGTGTCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATa         <  2:433429/150‑1 (MQ=255)                                                                                                                                                gtCTGCTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGttt  <  1:647214/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CGATCCGTTTTATCGTGAAGATTTCACGGTCCTCTACCGCTGGCCTCACGCTTTACTTAGCAATGCCTCGGCTCCGCAAACGACGAATATTTTCAGCCATCTTCTTTTACCCTCCACGTCATCGTTGTTAATTTGGTTTCCGTGTCTGTTCCTGGTTTGTGGATGGTTATATCTCTGGAAGCGCAGGCCGCAATTTACGCCGGTAGATGTGATTGAAACCGATGTCATTCCGCCAGATTACACGCCCGGCATGTTACGTCTCGATGCGAAGCTAGTTTACGACGATAAAGGTTT  >  NC_000913/1325366‑1325659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑