Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,496,777	T→C	intergenic (+146/‑79)	ydcJ → / → opgD	putative metalloenzyme/OPG biosynthetic periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,496,777	0	T	C	100.0%	325.8 / NA	86	intergenic (+146/‑79)	ydcJ/opgD	putative metalloenzyme/OPG biosynthetic periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/38);  total (48/38)

AAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTG  >  NC_000913/1496630‑1496922                                                                                                                                                    |                                                                                                                                                  aaaaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCat                                                                                                                                                <  2:412120/151‑1 (MQ=255) aaaaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCa                                                                                                                                                 <  1:651279/150‑1 (MQ=255)  aaaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCata                                                                                                                                               <  1:988659/151‑1 (MQ=255)   aaTCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCataa                                                                                                                                              <  1:339728/151‑1 (MQ=255)       tGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCataaata                                                                                                                                           >  2:653468/1‑150 (MQ=255)          ccATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCa                                                                                                                                       <  2:415373/151‑1 (MQ=255)            aTCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACt                                                                                                                                     <  1:210790/151‑1 (MQ=255)              ccGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACttt                                                                                                                                   <  2:363749/151‑1 (MQ=255)                gCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCt                                                                                                                                 >  1:157237/1‑151 (MQ=255)                 ccTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCtt                                                                                                                                >  1:847017/1‑151 (MQ=255)                 ccTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCtt                                                                                                                                <  1:1077480/151‑1 (MQ=255)                    ttGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTg                                                                                                                               <  1:755881/149‑1 (MQ=255)                        aaaaaTTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTg                                                                                                                         >  1:563337/1‑151 (MQ=255)                         aaaaTTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTg                                                                                                                         >  2:553736/1‑150 (MQ=255)                         aaaaTTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTg                                                                                                                         >  2:1079441/1‑150 (MQ=255)                                    gATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAg                                                                                                              >  1:529127/1‑150 (MQ=255)                                     aTTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAgcg                                                                                                            >  1:130404/1‑151 (MQ=255)                                     aTTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAgcg                                                                                                            >  1:1140285/1‑151 (MQ=255)                                         tACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCgg                                                                                                         <  1:394606/150‑1 (MQ=255)                                          aCAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGa                                                                                                       <  1:812582/151‑1 (MQ=255)                                           cAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGAc                                                                                                      <  2:95996/151‑1 (MQ=255)                                            aaaCGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGAcc                                                                                                     <  1:137987/151‑1 (MQ=255)                                            aaaCGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGAcc                                                                                                     >  2:916137/1‑151 (MQ=255)                                                gAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTc                                                                                                 <  1:725834/151‑1 (MQ=255)                                                 aaTCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTca                                                                                                <  2:786701/151‑1 (MQ=255)                                                  aTCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTcac                                                                                               >  2:56442/1‑151 (MQ=255)                                                       gCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAacac                                                                                          >  2:138917/1‑151 (MQ=255)                                                          cATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACAcc                                                                                         <  1:122570/149‑1 (MQ=255)                                                           aTCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGa                                                                                      <  2:19942/151‑1 (MQ=255)                                                           aTCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGa                                                                                      <  2:429011/151‑1 (MQ=255)                                                             cccATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAg                                                                                    <  1:978993/151‑1 (MQ=255)                                                              ccATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAgg                                                                                   <  2:509513/151‑1 (MQ=255)                                                                       tAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACttt                                                                          >  1:1152429/1‑151 (MQ=255)                                                                        aaaaaaaaTGCCGATTTATGCATATTCTCTCAGTTCATCAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                         >  2:149725/1‑151 (MQ=255)                                                                        aaaaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                         >  1:438163/1‑151 (MQ=255)                                                                        aaaaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                         <  1:541380/151‑1 (MQ=255)                                                                        aaaaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                         >  2:705260/1‑151 (MQ=255)                                                                         aaaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                         >  1:376405/1‑150 (MQ=255)                                                                          aaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                         >  1:946715/1‑149 (MQ=255)                                                                          aaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTc                                                                         >  2:317871/1‑149 (MQ=255)                                                                          aaaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAg                                                                       <  1:1142282/151‑1 (MQ=255)                                                                           aaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAgg                                                                      <  2:157272/151‑1 (MQ=255)                                                                           aaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAgg                                                                      >  1:10242/1‑151 (MQ=255)                                                                           aaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAgg                                                                      <  2:868062/151‑1 (MQ=255)                                                                            aaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAgg                                                                      >  1:322652/1‑150 (MQ=255)                                                                                    gATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCg                                                             >  1:466220/1‑151 (MQ=255)                                                                                    gATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCg                                                             <  2:419523/151‑1 (MQ=255)                                                                                    gATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCg                                                             >  2:90830/1‑151 (MQ=255)                                                                                        tATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGt                                                            >  2:603001/1‑148 (MQ=255)                                                                                         aTGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGAc                                                        <  1:156132/151‑1 (MQ=255)                                                                                               atTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGAttt                                                   >  2:869889/1‑150 (MQ=255)                                                                                               atTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGAttt                                                   >  1:114093/1‑150 (MQ=255)                                                                                                 tctctcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATttat                                                 <  1:506203/150‑1 (MQ=255)                                                                                                    ctcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATttatta                                               <  2:375976/149‑1 (MQ=255)                                                                                                    ctcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATttatt                                                <  1:99353/148‑1 (MQ=255)                                                                                                     tcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTaa                                              >  2:615285/1‑149 (MQ=255)                                                                                                      cAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTaa                                              <  1:257211/148‑1 (MQ=255)                                                                                                       aGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGt                                          >  1:600101/1‑151 (MQ=255)                                                                                                        gTTCAACAATTGGATTATTAATAAATATTCTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGtt                                         >  1:485725/1‑151 (MQ=255)                                                                                                           caacaaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCa                                       >  2:506916/1‑150 (MQ=255)                                                                                                           caacaaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCa                                       >  2:656612/1‑150 (MQ=255)                                                                                                            aacaaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAAt                                     >  1:357262/1‑151 (MQ=255)                                                                                                               aaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGc                                  >  1:904067/1‑151 (MQ=255)                                                                                                                   ggATTATTAATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTAt                               <  1:105860/150‑1 (MQ=255)                                                                                                                        attaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgccgc                         >  2:1164500/1‑151 (MQ=255)                                                                                                                         ttaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgccgcc                        >  2:408874/1‑151 (MQ=255)                                                                                                                         ttaATAAATATTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgccgcc                        >  2:186772/1‑151 (MQ=255)                                                                                                                           aataaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgt                      >  2:1042901/1‑151 (MQ=255)                                                                                                                           aataaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGCATGGATCGTCGACGATTTATTAAAGGATCAATGGCTATGGccgccg                       >  2:141735/1‑150 (MQ=255)                                                                                                                           aataaataTTGTCAAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAATGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGTTATGGCCGCCgt                      <  1:812214/151‑1 (MQ=255)                                                                                                                            ataaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgtg                     <  1:138882/151‑1 (MQ=255)                                                                                                                             taaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgtg                     >  1:919614/1‑150 (MQ=255)                                                                                                                             taaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgtg                     >  1:774827/1‑150 (MQ=255)                                                                                                                              aaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGtgtg                   >  2:16592/1‑151 (MQ=255)                                                                                                                              aaataTTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGtgtg                   <  1:746778/151‑1 (MQ=255)                                                                                                                                atatTGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCg                 >  1:15062/1‑151 (MQ=255)                                                                                                                                   ttGTCTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGt               >  1:207982/1‑150 (MQ=255)                                                                                                                                       cTAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTAcc            >  2:967106/1‑149 (MQ=255)                                                                                                                                        tAGAGTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGc         >  2:340979/1‑151 (MQ=255)                                                                                                                                         agagTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCg        >  1:603479/1‑151 (MQ=255)                                                                                                                                         agagTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGACTATGGCCGCCGTGTGCGGTACCAg          <  1:1079191/149‑1 (MQ=255)                                                                                                                                           agTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATg                              <  2:1127477/127‑1 (MQ=255)                                                                                                                                           agTGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATg                              >  1:1127225/1‑127 (MQ=255)                                                                                                                                             tGAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCAt    >  2:831008/1‑151 (MQ=255)                                                                                                                                              gAGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCAt    <  2:965475/150‑1 (MQ=255)                                                                                                                                               aGCGGCCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTg  <  2:897155/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  AAAATCTGACCATCCGCCTTTGCAAAAATTTGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTG  >  NC_000913/1496630‑1496922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑