Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	187,251	G→A	T467M (ACG→ATG)	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	187,251	0	G	A	98.7%	293.1 / ‑4.1	76	T467M (ACG→ATG)	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (40/35);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

ATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTT  >  NC_000913/187104‑187395                                                                                                                                                    |                                                                                                                                                 atatCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCatgt                                                                                                                                               >  2:557883/1‑151 (MQ=255)        gAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTg                                                                                                                                        >  2:678380/1‑151 (MQ=255)           aCAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCTAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGaa                                                                                                                                     <  2:1004583/151‑1 (MQ=255)                gcggcgATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGAt                                                                                                                                <  2:125991/151‑1 (MQ=255)                 cggcgATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATc                                                                                                                               <  2:390947/151‑1 (MQ=255)                  ggcgATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGAt                                                                                                                                <  2:938566/149‑1 (MQ=255)                   gcgATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCa                                                                                                                              >  2:1012360/1‑150 (MQ=255)                       tGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTg                                                                                                                         >  1:595865/1‑151 (MQ=255)                           aaTCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATc                                                                                                                     <  2:414351/151‑1 (MQ=255)                            aTCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGAGCAAACTGCATCt                                                                                                                    <  2:988740/151‑1 (MQ=255)                             tCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTg                                                                                                                   >  1:955126/1‑151 (MQ=255)                              cAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTg                                                                                                                   <  1:363653/150‑1 (MQ=255)                                gCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGccc                                                                                                                <  2:472679/151‑1 (MQ=255)                                   cAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCcgac                                                                                                             <  1:389272/151‑1 (MQ=255)                                   cAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCcg                                                                                                               <  2:534280/149‑1 (MQ=255)                                    aGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCcgacg                                                                                                            >  1:888414/1‑151 (MQ=255)                                       tGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGat                                                                                                          <  2:405154/150‑1 (MQ=255)                                       tGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGat                                                                                                          >  2:507106/1‑150 (MQ=255)                                        gACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGatat                                                                                                        >  2:1006725/1‑151 (MQ=255)                                         aCGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATg                                                                                                       <  1:41907/151‑1 (MQ=255)                                            gCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCg                                                                                                     >  1:192575/1‑150 (MQ=255)                                              aGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGatagatcgga                                                                                                  <  1:961070/151‑8 (MQ=255)                                              aGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGAcc                                                                                                  >  1:619420/1‑151 (MQ=255)                                                 cgcgGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCAtt                                                                                               <  1:384071/151‑1 (MQ=255)                                                  gcgGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCATTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTg                                                                                              <  1:15638/151‑1 (MQ=255)                                                   cgGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGc                                                                                             <  2:465473/151‑1 (MQ=255)                                                     gCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCgg                                                                                           <  2:60742/151‑1 (MQ=255)                                                     gCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCgg                                                                                           >  2:150941/1‑151 (MQ=255)                                                      ccAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGc                                                                                          <  2:546364/151‑1 (MQ=255)                                                      ccAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGc                                                                                          >  1:642146/1‑151 (MQ=255)                                                        aGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCat                                                                                        >  2:1091746/1‑151 (MQ=255)                                                        aGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCat                                                                                        >  2:309727/1‑151 (MQ=255)                                                        aGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCat                                                                                        >  1:841758/1‑151 (MQ=255)                                                          aCGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCatat                                                                                      >  1:164873/1‑151 (MQ=255)                                                           cGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCAtata                                                                                     <  2:856687/151‑1 (MQ=255)                                                            gTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCAtata                                                                                     >  2:5977/1‑150 (MQ=255)                                                            gTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATAc                                                                                    >  2:692994/1‑151 (MQ=255)                                                               cacacacaACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATAcgcg                                                                                 >  2:789878/1‑151 (MQ=255)                                                                     caACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGc                                                                           <  2:986299/151‑1 (MQ=255)                                                                            tggcgctggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTAt                                                                    >  2:880149/1‑151 (MQ=255)                                                                             ggcgctggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATg                                                                   <  2:978702/151‑1 (MQ=255)                                                                               cgctggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGc                                                                 <  1:702300/151‑1 (MQ=255)                                                                                  tggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGc                                                                 <  2:1178285/148‑1 (MQ=255)                                                                                   ggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATg                                                             <  1:514433/151‑1 (MQ=255)                                                                                      gcgTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCAt                                                          <  1:1081839/151‑1 (MQ=255)                                                                                       cgTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCAtt                                                         >  2:1177254/1‑151 (MQ=255)                                                                                       cgTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCAtt                                                         >  1:468726/1‑151 (MQ=255)                                                                                        gTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTg                                                        <  1:392695/151‑1 (MQ=255)                                                                                             ttcACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAAt                                                   >  2:826534/1‑151 (MQ=255)                                                                                               cACTGGCAGAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAAt                                                   >  1:455514/1‑149 (MQ=255)                                                                                                  tGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTAGGGCGATGCATTGGCAATAGccc                                              >  1:466597/1‑151 (MQ=255)                                                                                                       aaaCTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGcgcc                                         >  1:768367/1‑151 (MQ=255)                                                                                                            ctcCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCAcc                                    >  1:223269/1‑151 (MQ=255)                                                                                                              ccAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCg                                   <  1:483340/150‑1 (MQ=255)                                                                                                                  tttCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCg                              >  2:964362/1‑151 (MQ=255)                                                                                                                    tCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCgg                             <  1:631722/150‑1 (MQ=255)                                                                                                                     cAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCggg                            <  1:870377/150‑1 (MQ=255)                                                                                                                      aGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGt                          >  2:241515/1‑151 (MQ=255)                                                                                                                         aTCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGAc                       >  1:224899/1‑151 (MQ=255)                                                                                                                           cACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGc                     >  2:945537/1‑151 (MQ=255)                                                                                                                            aCGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGc                     >  2:1010812/1‑150 (MQ=255)                                                                                                                            aCGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCa                    <  2:919530/151‑1 (MQ=255)                                                                                                                             cgcgGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGc                     >  2:189025/1‑149 (MQ=255)                                                                                                                               cgGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGaa                 <  1:150905/151‑1 (MQ=255)                                                                                                                                ggATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAAt                >  2:985787/1‑151 (MQ=255)                                                                                                                                  aTAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGc              >  2:632881/1‑151 (MQ=255)                                                                                                                                   tAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAg                   <  2:181217/145‑1 (MQ=255)                                                                                                                                   tAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAg                   >  1:181178/1‑145 (MQ=255)                                                                                                                                   tAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGc              <  1:903468/150‑1 (MQ=255)                                                                                                                                   tAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCt             >  2:241/1‑151 (MQ=255)                                                                                                                                    aGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTc            >  2:509029/1‑151 (MQ=255)                                                                                                                                     gTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCa           <  2:1081222/151‑1 (MQ=255)                                                                                                                                     gTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCa           <  2:32296/151‑1 (MQ=255)                                                                                                                                       aTGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCaaa         <  2:455625/151‑1 (MQ=255)                                                                                                                                       aTGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCaa          >  1:730050/1‑150 (MQ=255)                                                                                                                                         gTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCaaaa        >  1:396977/1‑150 (MQ=255)                                                                                                                                              tCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGtt  >  2:506877/1‑151 (MQ=255)                                                                                                                                               ccaccaTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGtt  <  1:689981/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 ATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTT  >  NC_000913/187104‑187395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑