Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,784	A→G	S356S (AGT→AGC)	uidC ←	putative outer membrane porin for beta‑glucuronides porin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,784	0	A	G	100.0%	301.9 / NA	80	S356S (AGT→AGC)	uidC	putative outer membrane porin for beta‑glucuronides porin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/40);  total (40/40)

CAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTACTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCC  >  NC_000913/1691637‑1691931                                                                                                                                                    |                                                                                                                                                    cAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCt                                                                                                                                                   <  2:386378/150‑1 (MQ=255)      gATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTc                                                                                                                                             >  1:346408/1‑151 (MQ=255)       aTCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGTTTc                                                                                                                                             >  2:488356/1‑150 (MQ=255)          gCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCAt                                                                                                                                           >  1:856796/1‑149 (MQ=255)             cAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATa                                                                                                                                          >  2:458981/1‑147 (MQ=255)                 gTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCcgcg                                                                                                                                    >  1:818358/1‑149 (MQ=255)                   aCTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGt                                                                                                                                 <  2:953072/150‑1 (MQ=255)                   aCTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTc                                                                                                                                <  1:595990/151‑1 (MQ=255)                    cTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTc                                                                                                                                >  1:190291/1‑150 (MQ=255)                     tGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTcac                                                                                                                              >  2:28256/1‑151 (MQ=255)                      gCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCTCCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTcaca                                                                                                                             <  2:696343/151‑1 (MQ=255)                            gcgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGCCCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCCTTTGCTGGATGCATAGCCGCGAGTCACAATCg                                                                                                                         <  1:942592/149‑1 (MQ=255)                            gcgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCgg                                                                                                                        >  2:608551/1‑150 (MQ=255)                             cgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTcaca                                                                                                                             >  2:1075323/1‑144 (MQ=255)                             cgcTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTcaca                                                                                                                             <  1:1075074/144‑1 (MQ=255)                                   tCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAAt                                                                                                                 <  1:462983/150‑1 (MQ=255)                                        cccTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGc                                                                                                           <  2:1185614/151‑1 (MQ=255)                                        cccTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGc                                                                                                           <  1:964383/151‑1 (MQ=255)                                           tttATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGt                                                                                                         >  1:503198/1‑150 (MQ=255)                                            ttATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGt                                                                                                         <  2:688277/149‑1 (MQ=255)                                            ttATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTa                                                                                                        <  1:929766/150‑1 (MQ=255)                                              aTCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAAt                                                                                                     <  2:1117903/151‑1 (MQ=255)                                                  gcagcaAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTgcg                                                                                                 <  2:1135367/151‑1 (MQ=255)                                                     gcaAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTgcggcg                                                                                              <  2:341384/151‑1 (MQ=255)                                                      caAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCCGGCTTCTTCTTGAAAAAACACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGt                                                                                             >  2:26118/1‑151 (MQ=255)                                                         gaaagaaaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTaa                                                                                          <  2:467370/151‑1 (MQ=255)                                                           aagaaaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAAc                                                                                        <  2:314134/151‑1 (MQ=255)                                                           aagaaaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAAc                                                                                        <  2:1010813/151‑1 (MQ=255)                                                            agaaaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACg                                                                                       <  2:808543/151‑1 (MQ=255)                                                             gaaaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACg                                                                                       >  1:631697/1‑150 (MQ=255)                                                             gaaaTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACg                                                                                       >  2:208383/1‑150 (MQ=255)                                                                aTTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAg                                                                                   <  2:420612/151‑1 (MQ=255)                                                                 ttCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGt                                                                                  >  2:987935/1‑151 (MQ=255)                                                                  tCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGtt                                                                                 >  2:104238/1‑151 (MQ=255)                                                                      cccTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGAc                                                                              <  2:1076418/150‑1 (MQ=255)                                                                          tGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATTTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGccc                                                                          >  2:137876/1‑150 (MQ=255)                                                                          tGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTATGCTGGCTTCATAGCCGCGAGTCACAATAGGTGCATATGTCAGCGTATCTTGCAGCGTTAAACGATAGTTGACGcc                                                                           >  2:895067/1‑149 (MQ=255)                                                                                ggCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGCTTCTTATCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGcc                                                                   >  2:402385/1‑151 (MQ=255)                                                                                ggCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATATCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGcc                                                                   >  2:271543/1‑151 (MQ=255)                                                                                   ccTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAgg                                                                >  2:315568/1‑151 (MQ=255)                                                                                    cTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAgg                                                                >  1:347210/1‑150 (MQ=255)                                                                                      tCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACt                                                             >  1:287476/1‑151 (MQ=255)                                                                                         cTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGc                                                           >  1:759115/1‑150 (MQ=255)                                                                                          tGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCAt                                                         >  1:664005/1‑151 (MQ=255)                                                                                           gACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCAtt                                                        <  2:280669/151‑1 (MQ=255)                                                                                            aCCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTc                                                       >  1:671326/1‑151 (MQ=255)                                                                                              ccGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCt                                                     <  2:857410/151‑1 (MQ=255)                                                                                                gATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTc                                                   <  1:419944/151‑1 (MQ=255)                                                                                                 aTAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTc                                                   >  2:994660/1‑150 (MQ=255)                                                                                                      tCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGtt                                             >  1:788362/1‑151 (MQ=255)                                                                                                       cATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGtt                                             <  2:742139/150‑1 (MQ=255)                                                                                                        aTACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTAc                                           >  1:204870/1‑151 (MQ=255)                                                                                                          accaccTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACg                                          <  1:402288/150‑1 (MQ=255)                                                                                                          accaccTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGa                                         <  1:28251/151‑1 (MQ=255)                                                                                                           ccaccTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGAt                                        <  2:388532/151‑1 (MQ=255)                                                                                                            caccTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATc                                       >  2:279813/1‑151 (MQ=255)                                                                                                            caccTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATc                                       >  1:201123/1‑151 (MQ=255)                                                                                                              ccTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTACAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCaa                                     <  1:608407/151‑1 (MQ=255)                                                                                                                 aaGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAg                                    <  1:934944/149‑1 (MQ=255)                                                                                                                  aGATACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACt                                 <  2:1109615/151‑1 (MQ=255)                                                                                                                     tACCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTa                               >  1:1072644/1‑150 (MQ=255)                                                                                                                      aCCCGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTaa                              <  2:856994/150‑1 (MQ=255)                                                                                                                       cccGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAAt                             <  1:577580/150‑1 (MQ=255)                                                                                                                       cccGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAAt                             <  2:882490/150‑1 (MQ=255)                                                                                                                        ccGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATg                            >  2:1131542/1‑150 (MQ=255)                                                                                                                        ccGTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATg                            >  2:135965/1‑150 (MQ=255)                                                                                                                          gTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGaaa                         <  2:802245/151‑1 (MQ=255)                                                                                                                          gTGCCTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCACAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGaaa                         <  1:361589/151‑1 (MQ=255)                                                                                                                             ccTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAgg                      >  1:812002/1‑151 (MQ=255)                                                                                                                              cTTCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAggg                     >  2:952228/1‑151 (MQ=255)                                                                                                                                tCAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGa                    <  1:110011/150‑1 (MQ=255)                                                                                                                                 cAATCCGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATa                  <  2:787302/151‑1 (MQ=255)                                                                                                                                   aTCCTCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGc                >  2:203287/1‑151 (MQ=255)                                                                                                                                     ccGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCTAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCa              <  1:26113/151‑1 (MQ=255)                                                                                                                                      cGCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCa              >  1:317169/1‑150 (MQ=255)                                                                                                                                       gCACATCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAAt            <  1:571178/151‑1 (MQ=255)                                                                                                                                         acaTCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGt          >  1:129997/1‑151 (MQ=255)                                                                                                                                          caTCTCGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTc         <  1:742438/151‑1 (MQ=255)                                                                                                                                             ctcGTTTGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGt      >  2:620094/1‑151 (MQ=255)                                                                                                                                                 tttGCTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATcc  >  2:719005/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCTTCGCTGACCCGATAGTTCATACCACCTAAGATACCCGTGCCTTCAATCCGCACATCTCGTTTACTGGATTCATAGCCGCGAGTCACAATCGGTGCAAATGTCAGCGTAAATTGCGGCGTTAAACGATAGTTGACGCCCAATTGCCAGGACTGCATTCCTTCACGGTTACGATCAAGACTTAATGAAAAGGGATAGCCAATGTCGGTATCC  >  NC_000913/1691637‑1691931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑