Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,833,205	G→A	A260T (GCC→ACC)	xthA →	exonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,833,205	0	G	A	98.4%	222.7 / NA	65	A260T (GCC→ACC)	xthA	exonuclease III
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (30/33);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGCGTTTTT  >  NC_000913/1833057‑1833350                                                                                                                                                     |                                                                                                                                                  ctcATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACAcc                                                                                                                                                 >  2:588116/1‑151 (MQ=255)  tcATGGTTTGATTACCGCTCAAAAGGATTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACAccc                                                                                                                                                >  1:825084/1‑151 (MQ=255)    aTGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACAccccc                                                                                                                                              <  2:248097/151‑1 (MQ=255)      ggTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGt                                                                                                                                            <  1:1097671/151‑1 (MQ=255)           gATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTggg                                                                                                                                       >  1:87575/1‑151 (MQ=255)              tACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCg                                                                                                                                     >  2:648228/1‑150 (MQ=255)                    tCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTcc                                                                                                                              >  1:133383/1‑151 (MQ=255)                              ttGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAAt                                                                                                                     <  2:21384/150‑1 (MQ=255)                               tGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAAttt                                                                                                                   >  1:709275/1‑151 (MQ=255)                                gACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAAttt                                                                                                                   >  2:1156084/1‑150 (MQ=255)                                    aTAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTagc                                                                                                                >  2:153843/1‑149 (MQ=255)                                      aaCCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTagcagc                                                                                                             >  1:344140/1‑150 (MQ=255)                                      aaCCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGct                                                                                                            >  2:515562/1‑151 (MQ=255)                                         cGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCtctc                                                                                                         >  1:662494/1‑151 (MQ=255)                                          gTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTcc                                                                                                        >  2:527453/1‑151 (MQ=255)                                                 gcgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCt                                                                                                   <  1:259021/149‑1 (MQ=255)                                                 gcgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTc                                                                                                  >  1:819176/1‑150 (MQ=255)                                                 gcgcATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCa                                                                                                 <  2:139089/151‑1 (MQ=255)                                                       cGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTgg                                                                                           <  1:700211/151‑1 (MQ=255)                                                       cGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTg                                                                                            <  1:14603/150‑1 (MQ=255)                                                             gctgctCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCa                                                                                       <  1:113798/149‑1 (MQ=255)                                                             gctgctCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCa                                                                                       <  1:192750/149‑1 (MQ=255)                                                             gctgctCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCa                                                                                       <  1:1115431/149‑1 (MQ=255)                                                                 ctCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGgaga                                                                                  <  1:1079757/150‑1 (MQ=255)                                                                 cccGCCAGCCAACCGCTGGCAGAATTTTGCGTAGAAACCTGCATCGACTATGAAATCCGCAGCATGGAAAACCCGTCCGATCACCCCCCCGTCCGGGCGATCTTCCGCCCCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGgaga                                                                                  <  1:766786/148‑1 (MQ=255)                                                                   cGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGaa                                                                                 <  2:587640/149‑1 (MQ=255)                                                                   cGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAAtt                                                                               <  2:1134983/151‑1 (MQ=255)                                                                    gccagccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTa                                                                              <  2:546752/151‑1 (MQ=255)                                                                        gccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCt                                                                          <  2:422244/151‑1 (MQ=255)                                                                         ccaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCtt                                                                         >  1:588585/1‑151 (MQ=255)                                                                         acaACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCtt                                                                         >  2:212547/2‑151 (MQ=255)                                                                          caACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTg                                                                        <  2:782910/151‑1 (MQ=255)                                                                          caACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTg                                                                        <  1:90821/151‑1 (MQ=255)                                                                           aaCCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTga                                                                       <  1:1094970/151‑1 (MQ=255)                                                                             ccGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTgaga                                                                     <  1:74896/151‑1 (MQ=255)                                                                              cGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTgaga                                                                     >  2:94433/1‑150 (MQ=255)                                                                               gCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGaaa                                                                   <  1:610899/151‑1 (MQ=255)                                                                               gCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGaa                                                                    >  1:20689/1‑150 (MQ=255)                                                                                 tGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGaaaa                                                                  >  2:1172796/1‑150 (MQ=255)                                                                                     aGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATca                                                              >  1:725901/1‑150 (MQ=255)                                                                                           ttGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACt                                                       >  2:164131/1‑151 (MQ=255)                                                                                            tGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACt                                                       <  1:796645/150‑1 (MQ=255)                                                                                              cGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTc                                                    <  1:71097/151‑1 (MQ=255)                                                                                                 aGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAg                                                  <  2:442094/150‑1 (MQ=255)                                                                                                  gAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTa                                                <  2:345738/151‑1 (MQ=255)                                                                                                      ccGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATg                                             >  1:557198/1‑150 (MQ=255)                                                                                                         gCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGAttt                                         <  1:935240/151‑1 (MQ=255)                                                                                                             cGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATttgtt                                      <  2:87599/150‑1 (MQ=255)                                                                                                                  aTGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgcctgc                                >  2:281890/1‑151 (MQ=255)                                                                                                                  aTGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgcctg                                 <  2:1174834/150‑1 (MQ=255)                                                                                                                  aTGAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTtgcctg                                 <  2:191539/150‑1 (MQ=255)                                                                                                                    gAAATCCGCAGCATGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCg                              <  1:592560/151‑1 (MQ=255)                                                                                                                           gcagcaTGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTg                       >  2:346974/1‑151 (MQ=255)                                                                                                                            cagcaTGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGt                      >  2:126837/1‑151 (MQ=255)                                                                                                                            cagcaTGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGt                      >  2:721733/1‑151 (MQ=255)                                                                                                                             agcaTGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGt                      <  2:825271/150‑1 (MQ=255)                                                                                                                                aTGGAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCTTCCTTTGTtat                   <  2:981462/150‑1 (MQ=255)                                                                                                                                   gAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCg               >  2:785633/1‑151 (MQ=255)                                                                                                                                   gAAAAACCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCg               >  2:791999/1‑151 (MQ=255)                                                                                                                                     aaaaCCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGtc             <  1:1162520/151‑1 (MQ=255)                                                                                                                                      aaaCCGTCCGATCCCACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGtct            <  1:200673/151‑1 (MQ=255)                                                                                                                                        aCCGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTctct          <  1:527321/151‑1 (MQ=255)                                                                                                                                          cGTCCGATCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGc        >  2:1154674/1‑151 (MQ=255)                                                                                                                                                aTCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGCGttttt  >  1:244054/1‑151 (MQ=255)                                                                                                                                                 tCACACCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGCGttttt  >  1:1086818/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTGGCAGAATGTTGCGTAGAAACCGGCATCGACTATGAAATCCGCAGCATGGAAAAACCGTCCGATCACGCCCCCGTCTGGGCGACCTTCCGCCGCTAATTTAGCAGCTCTCCTGGCTCAAACTGGGTCAGGAGAATTAACCTTGAGAAAAATCAACAAACTGTCAGTAATGATTTGTTGCCTGCCGTCCTTTGTTATACCGTCTCTGCGTTTTT  >  NC_000913/1833057‑1833350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑