Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,140,009	T→C	D535G (GAC→GGC)	asmA ←	suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,140,009	0	T	C	100.0%	300.1 / NA	83	D535G (GAC→GGC)	asmA	suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/40);  total (43/40)

TTGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAA  >  NC_000913/2139862‑2140154                                                                                                                                                    |                                                                                                                                                  ttGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGc                                                                                                                                                >  1:882191/1‑151 (MQ=255)   gcgcAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCa                                                                                                                                               <  1:1068933/150‑1 (MQ=255)   gcgcAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAg                                                                                                                                              <  2:158472/151‑1 (MQ=255)    cgcAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAgg                                                                                                                                             >  1:632276/1‑151 (MQ=255)       aGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGttt                                                                                                                                          >  2:96361/1‑151 (MQ=255)          aaCTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGa                                                                                                                                        <  1:1158810/150‑1 (MQ=255)          aaCTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGa                                                                                                                                        <  2:807976/150‑1 (MQ=255)          aaCTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGa                                                                                                                                        <  1:198490/150‑1 (MQ=255)              gATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGc                                                                                                                                   >  1:678431/1‑151 (MQ=255)              gATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGc                                                                                                                                   >  2:344772/1‑151 (MQ=255)                 ccACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCa                                                                                                                                 <  1:825057/150‑1 (MQ=255)                 ccACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCa                                                                                                                                 <  2:279841/150‑1 (MQ=255)                  cACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGa                                                                                                                               <  1:1007118/151‑1 (MQ=255)                      tGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGAtt                                                                                                                             <  2:1157179/149‑1 (MQ=255)                       gCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCa                                                                                                                           <  2:1024099/150‑1 (MQ=255)                          ggCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACAt                                                                                                                       >  2:1008543/1‑151 (MQ=255)                                 aTTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTcc                                                                                                                >  1:1074254/1‑151 (MQ=255)                                           tgcCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAgcg                                                                                                      >  2:219495/1‑151 (MQ=255)                                             ccAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAAACAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGcc                                                                                                    >  1:108181/1‑151 (MQ=255)                                               aGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGcc                                                                                                    >  1:763507/1‑149 (MQ=255)                                               aGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAg                                                                                                  <  1:292699/151‑1 (MQ=255)                                               aGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAg                                                                                                  <  2:618104/151‑1 (MQ=255)                                                gTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGc                                                                                                 <  2:136919/151‑1 (MQ=255)                                                 ttGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCTCCTGTCAGCGCCAGc                                                                                                 >  1:27286/1‑150 (MQ=255)                                                   gCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGcaca                                                                                              <  1:985005/151‑1 (MQ=255)                                                   gCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGcac                                                                                               <  1:1162307/150‑1 (MQ=255)                                                    ccATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAg                                                                                             <  1:765561/151‑1 (MQ=255)                                                      aTAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGc                                                                                           >  1:900415/1‑151 (MQ=255)                                                      aTAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGc                                                                                           >  1:653823/1‑151 (MQ=255)                                                        aaaCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCg                                                                                          <  1:958588/150‑1 (MQ=255)                                                        aaaCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCg                                                                                          <  1:64493/150‑1 (MQ=255)                                                         aaCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGAt                                                                                        >  2:746111/1‑151 (MQ=255)                                                            ccGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTg                                                                                      >  2:759879/1‑150 (MQ=255)                                                               cAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCt                                                                                  >  2:485467/1‑151 (MQ=255)                                                                aGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACTACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCtt                                                                                 <  1:669622/151‑1 (MQ=255)                                                                aGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCtt                                                                                 >  2:495439/1‑151 (MQ=255)                                                                  cGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGc                                                                               <  2:563971/151‑1 (MQ=255)                                                                     tACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATg                                                                            >  1:603837/1‑151 (MQ=255)                                                                      aCTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGt                                                                           >  2:733919/1‑151 (MQ=255)                                                                      aCTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGt                                                                           >  1:594910/1‑151 (MQ=255)                                                                        tGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCg                                                                         >  1:218556/1‑151 (MQ=255)                                                                          gCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGtt                                                                       >  2:146275/1‑151 (MQ=255)                                                                           cGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGttt                                                                      >  1:782661/1‑151 (MQ=255)                                                                            gTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCCCAGGCGATTGACCTTGCATGTCGtt                                                                       >  1:252469/1‑149 (MQ=255)                                                                             tttctttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTa                                                                     >  1:899452/1‑150 (MQ=255)                                                                                  ttcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACg                                                                  <  2:95613/148‑1 (MQ=255)                                                                                   tcAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCac                                                              >  2:1146128/1‑151 (MQ=255)                                                                                    cAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCacg                                                             <  2:678591/151‑1 (MQ=255)                                                                                     aGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCacg                                                             >  1:889903/1‑150 (MQ=255)                                                                                        aaaTCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGc                                                         >  1:1163334/1‑151 (MQ=255)                                                                                        aaaTCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGc                                                         >  1:254640/1‑151 (MQ=255)                                                                                         aatcaatcAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGcc                                                        >  2:752474/1‑151 (MQ=255)                                                                                               tcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCtt                                                  >  2:906511/1‑151 (MQ=255)                                                                                               tcaGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCtt                                                  >  1:935187/1‑151 (MQ=255)                                                                                                caGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCtt                                                  <  1:593247/150‑1 (MQ=255)                                                                                                  gTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCaa                                               <  1:843313/151‑1 (MQ=255)                                                                                                       cTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTc                                           >  2:207611/1‑150 (MQ=255)                                                                                                            cccGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCg                                     <  1:1189220/151‑1 (MQ=255)                                                                                                                 tCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtggt                                >  2:75518/1‑151 (MQ=255)                                                                                                                 tCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtggt                                >  1:798146/1‑151 (MQ=255)                                                                                                                   cAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTaa                              <  2:734318/151‑1 (MQ=255)                                                                                                                    aGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTaaa                             <  2:533048/151‑1 (MQ=255)                                                                                                                     gCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTaaa                             <  1:598140/150‑1 (MQ=255)                                                                                                                      ccacccacGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGc                           >  1:715719/1‑151 (MQ=255)                                                                                                                         cccacGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCgg                         >  2:775433/1‑150 (MQ=255)                                                                                                                            acGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCa                      >  1:217500/1‑150 (MQ=255)                                                                                                                             cGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCa                      >  2:205092/1‑149 (MQ=255)                                                                                                                             cGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAg                    <  1:1083599/151‑1 (MQ=255)                                                                                                                               aCCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGc                   <  1:202780/150‑1 (MQ=255)                                                                                                                                cccGAATATCAAGCTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCTTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcg                 <  2:334625/151‑1 (MQ=255)                                                                                                                                cccGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcg                 <  1:623686/151‑1 (MQ=255)                                                                                                                                 ccGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcgc                <  2:897162/151‑1 (MQ=255)                                                                                                                                 ccGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcgc                <  1:476037/151‑1 (MQ=255)                                                                                                                                 ccGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcgc                <  1:964712/151‑1 (MQ=255)                                                                                                                                   gAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGt              <  1:1145869/151‑1 (MQ=255)                                                                                                                                   gAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGt              <  1:364860/151‑1 (MQ=255)                                                                                                                                   gAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGt              >  1:874567/1‑151 (MQ=255)                                                                                                                                      tatCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCAc           >  1:1014384/1‑151 (MQ=255)                                                                                                                                       atCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACg          >  2:386892/1‑151 (MQ=255)                                                                                                                                       atCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACg          <  2:1178882/151‑1 (MQ=255)                                                                                                                                       atCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACg          <  1:499801/151‑1 (MQ=255)                                                                                                                                               gggTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCaaa  <  2:839224/151‑1 (MQ=255)                                                                                                                                               gggTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCaa   >  1:174214/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TTGCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAA  >  NC_000913/2139862‑2140154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑