Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,536,495 G→A A248V (GCG→GTG)  pdxK ← pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,4950GA98.8% 317.6 / NA 86A248V (GCG→GTG) pdxKpyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (41/44);  minor base T (0/1);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTAC  >  NC_000913/2536348‑2536642
                                                                                                                                                   |                                                                                                                                                   
tGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCt                                                                                                                                                  >  1:675142/1‑151 (MQ=255)
   cGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTcc                                                                                                                                               <  2:434748/151‑1 (MQ=255)
         gAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTc                                                                                                                                          <  1:502150/150‑1 (MQ=255)
           aaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGc                                                                                                                                       >  1:1098491/1‑151 (MQ=255)
           aaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGc                                                                                                                                       >  1:230406/1‑151 (MQ=255)
             aaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCa                                                                                                                                      >  1:472452/1‑150 (MQ=255)
                ggCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGc                                                                                                                                   <  2:382287/150‑1 (MQ=255)
                      cATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgat                                                                                                                            >  2:699278/1‑151 (MQ=255)
                      cATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgat                                                                                                                            <  1:150544/151‑1 (MQ=255)
                       aTCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACtgatg                                                                                                                           >  2:252406/1‑151 (MQ=255)
                           gcgcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagct                                                                                                                       >  2:484488/1‑151 (MQ=255)
                             gcCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctga                                                                                                                     <  2:341899/151‑1 (MQ=255)
                              ccATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgag                                                                                                                    <  2:423992/151‑1 (MQ=255)
                              ccATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctgag                                                                                                                    <  1:1128997/151‑1 (MQ=255)
                                  ttttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagctg                                                                                                                      <  1:146235/145‑1 (MQ=255)
                                  ttttttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGAtgagct                                                                                                                       >  2:146271/1‑144 (MQ=255)
                                      ttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACaaaa                                                                                                             <  2:144699/150‑1 (MQ=255)
                                      ttATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAAc                                                                                                            >  1:371937/1‑151 (MQ=255)
                                       tATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAAc                                                                                                            <  2:575984/150‑1 (MQ=255)
                                        aTGTTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGATCGGTGCTCTGCATCGGTTAACTCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACaaa                                                                                                              <  1:552730/147‑1 (MQ=255)
                                        aTGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAAc                                                                                                            >  2:305219/1‑149 (MQ=255)
                                        aTGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAAc                                                                                                            >  1:127571/1‑149 (MQ=255)
                                        aTGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAg                                                                                                          <  2:404980/151‑1 (MQ=255)
                                        aTGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAg                                                                                                          <  2:230455/151‑1 (MQ=255)
                                          gCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGt                                                                                                        >  1:14985/1‑151 (MQ=255)
                                           cTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTc                                                                                                       >  2:589626/1‑151 (MQ=255)
                                             tCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTc                                                                                                       <  1:834961/149‑1 (MQ=255)
                                               cGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCa                                                                                                   <  1:1012311/151‑1 (MQ=255)
                                                  cAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTc                                                                                                <  1:793504/151‑1 (MQ=255)
                                                  cAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTc                                                                                                >  2:756524/1‑151 (MQ=255)
                                                   aGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTcc                                                                                               >  2:1025931/1‑151 (MQ=255)
                                                    gcggcgGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTccc                                                                                              <  2:545298/151‑1 (MQ=255)
                                                     cggcggCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCt                                                                                             <  1:1052905/151‑1 (MQ=255)
                                                        cggcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTc                                                                                          >  1:902115/1‑151 (MQ=255)
                                                          gcAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTc                                                                                          >  1:705177/1‑149 (MQ=255)
                                                            aaaaTCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAgg                                                                                       >  1:541902/1‑150 (MQ=255)
                                                                tCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGt                                                                                  >  2:924628/1‑151 (MQ=255)
                                                                 cAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGtt                                                                                 <  1:184621/151‑1 (MQ=255)
                                                                  aaTTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGttt                                                                                >  2:1104072/1‑151 (MQ=255)
                                                                     tCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGttttt                                                                              <  1:834834/150‑1 (MQ=255)
                                                                     tCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGttttt                                                                              <  2:466130/150‑1 (MQ=255)
                                                                      cATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTAc                                                                            <  1:10522/151‑1 (MQ=255)
                                                                           ctctCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTg                                                                       >  2:304619/1‑151 (MQ=255)
                                                                           ctctCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTg                                                                       >  2:410341/1‑151 (MQ=255)
                                                                            tctcATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTg                                                                       >  1:1108723/1‑150 (MQ=255)
                                                                             ctcATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGa                                                                      >  1:976997/1‑150 (MQ=255)
                                                                              tcATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAAt                                                                    >  2:120360/1‑151 (MQ=255)
                                                                                aTGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATgg                                                                  <  1:851389/151‑1 (MQ=255)
                                                                                aTGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATgg                                                                  >  2:699721/1‑151 (MQ=255)
                                                                                    tgctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGaa                                                               >  2:698404/1‑150 (MQ=255)
                                                                                     gctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGaa                                                               <  2:1060347/149‑1 (MQ=255)
                                                                                      ctgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATg                                                            >  2:705681/1‑151 (MQ=255)
                                                                                       tgGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATg                                                            >  2:738955/1‑150 (MQ=255)
                                                                                        gggTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATg                                                            <  1:899306/149‑1 (MQ=255)
                                                                                               gcgcATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACg                                                   <  1:373370/151‑1 (MQ=255)
                                                                                                    tCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTc                                              >  1:624548/1‑151 (MQ=255)
                                                                                                    tCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTc                                              >  1:214381/1‑151 (MQ=255)
                                                                                                      aCTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCgg                                            >  2:895712/1‑151 (MQ=255)
                                                                                                         tCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCa                                          <  2:717056/150‑1 (MQ=255)
                                                                                                         tCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCa                                          <  1:280904/150‑1 (MQ=255)
                                                                                                           cAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTg                                       <  1:925960/151‑1 (MQ=255)
                                                                                                           cAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTg                                       <  2:1098742/151‑1 (MQ=255)
                                                                                                            aGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGa                                      <  2:745079/151‑1 (MQ=255)
                                                                                                                cgcgCAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGaccac                                  <  2:967596/151‑1 (MQ=255)
                                                                                                                   gcAACCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACcaccac                               >  1:224863/1‑151 (MQ=255)
                                                                                                                     aaCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACcaca                              >  1:6127/1‑150 (MQ=255)
                                                                                                                      aCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAAc                            <  2:151914/151‑1 (MQ=255)
                                                                                                                      aCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAAc                            <  1:698236/151‑1 (MQ=255)
                                                                                                                      aCCCCGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAAc                            >  1:971291/1‑151 (MQ=255)
                                                                                                                       ccccGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTATCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAAcc                           >  1:485562/1‑151 (MQ=255)
                                                                                                                          cGCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAGAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGc                        <  1:647850/151‑1 (MQ=255)
                                                                                                                           gCTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGc                        >  1:574062/1‑150 (MQ=255)
                                                                                                                            cTCGGTGCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCAt                      >  1:560967/1‑151 (MQ=255)
                                                                                                                                  gCACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCt                 <  1:762683/150‑1 (MQ=255)
                                                                                                                                   cACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGa               <  1:851184/151‑1 (MQ=255)
                                                                                                                                   cACTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGa               <  1:713115/151‑1 (MQ=255)
                                                                                                                                     cTGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGAt              <  2:424720/150‑1 (MQ=255)
                                                                                                                                      tGCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGAttt            >  1:218453/1‑151 (MQ=255)
                                                                                                                                       gCATCGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGAttt            <  2:672973/150‑1 (MQ=255)
                                                                                                                                           cGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTttcttc       <  1:76729/151‑1 (MQ=255)
                                                                                                                                           cGGTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTttcttc       <  2:287938/151‑1 (MQ=255)
                                                                                                                                            ggTTAACACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTttcttc       >  1:399206/1‑150 (MQ=255)
                                                                                                                                                aaCACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTa   <  2:616697/150‑1 (MQ=255)
                                                                                                                                                aaCACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTa   <  2:14985/150‑1 (MQ=255)
                                                                                                                                                aaCACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTAc  >  2:802487/1‑151 (MQ=255)
                                                                                                                                                aaCACCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCCTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTa   <  1:1149795/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCACTGATGAGCTGAGCACAAAACAGGTCGCCAGTCCCTTTCAGGTCGGTTTTTACCCGTGAATGGGAAATGACATTCACGCTGTCGGCAGTGACCACCACAACCTGCATCTCCTGATTTTCTTCATTAC  >  NC_000913/2536348‑2536642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: