Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,845,283	T→C	S397G (AGC→GGC)	hycE ←	hydrogenase 3, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,845,283	0	T	C	100.0%	239.5 / NA	68	S397G (AGC→GGC)	hycE	hydrogenase 3, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/35);  total (33/35)

CGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCTGTTGTGCC  >  NC_000913/2845136‑2845428                                                                                                                                                    |                                                                                                                                                  cGATCATGTTCAGCGCGGTATAGACTTCGATGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGc                                                                                                                                                >  2:456489/1‑151 (MQ=255)      aTGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACgg                                                                                                                                            >  2:769297/1‑150 (MQ=255)      aTGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGa                                                                                                                                           <  2:720714/151‑1 (MQ=255)         ttCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGAcc                                                                                                                                         >  2:82993/1‑150 (MQ=255)           cAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCAt                                                                                                                                       <  2:55482/150‑1 (MQ=255)             gcgcGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCgg                                                                                                                                    >  1:879199/1‑151 (MQ=255)             gcgcGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCgg                                                                                                                                    <  1:729282/151‑1 (MQ=255)             gcgcGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCg                                                                                                                                     <  2:908667/150‑1 (MQ=255)                    ataGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCAATACCCGACAAACGGGTGATCGGCGCGGGTATCAGGGGGGTGACCGCCGGCACGGACCATCGGGCCGAc                                                                                                                              <  2:358164/150‑1 (MQ=255)                     taGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGt                                                                                                                            >  2:202772/1‑151 (MQ=255)                      aGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGtt                                                                                                                           <  2:717707/151‑1 (MQ=255)                       gACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTa                                                                                                                          <  1:1135102/151‑1 (MQ=255)                            cGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGa                                                                                                                      <  2:724462/150‑1 (MQ=255)                                gATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTcg                                                                                                                 >  2:544280/1‑151 (MQ=255)                                  tACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTcgcg                                                                                                               >  2:488621/1‑151 (MQ=255)                                   aCGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGGGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCgcga                                                                                                              <  2:571136/151‑1 (MQ=255)                                      cACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCgcgagcg                                                                                                           <  2:337055/151‑1 (MQ=255)                                       aCTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCgcgagcg                                                                                                           >  1:954152/1‑150 (MQ=255)                                       aCTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCgcgagcg                                                                                                           >  2:733011/1‑150 (MQ=255)                                        cTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGAt                                                                                                         >  2:950354/1‑151 (MQ=255)                                         tttCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCgcgagcga                                                                                                          <  2:572078/149‑1 (MQ=255)                                         tttCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGAtt                                                                                                        >  1:928394/1‑151 (MQ=255)                                          ttCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGAtt                                                                                                        <  1:922187/150‑1 (MQ=255)                                           tCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGAttt                                                                                                       <  2:540006/150‑1 (MQ=255)                                              gACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCgg                                                                                                   >  2:286374/1‑151 (MQ=255)                                                 ggtaaaTAACTGCCCAGCCCTCCTCGCGGTGGTATTCCATTGGCAGCAGACAATAGCCGACAAACGGGTGATCGGTGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGGGAGCGATTTCCGGGt                                                                                                 <  2:107958/147‑1 (MQ=255)                                                 gggAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTc                                                                                                <  2:852904/151‑1 (MQ=255)                                                        aaCGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAgacg                                                                                          >  2:927655/1‑150 (MQ=255)                                                         aCGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAgacgac                                                                                        >  1:793990/1‑151 (MQ=255)                                                          cGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAgacgac                                                                                        <  2:60374/150‑1 (MQ=255)                                                          cGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGAcc                                                                                       >  2:635616/1‑151 (MQ=255)                                                           gTCGCAGCCCTGCTCGATGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCa                                                                                      <  2:680452/151‑1 (MQ=255)                                                            tCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCa                                                                                      >  1:117848/1‑150 (MQ=255)                                                               cAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGc                                                                                  >  1:1039895/1‑151 (MQ=255)                                                                  cccTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGGGGGTATCACGGGGGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGa                                                                               <  2:1038557/151‑1 (MQ=255)                                                                  cccTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCg                                                                                >  1:811124/1‑150 (MQ=255)                                                                   ccTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGAc                                                                              >  1:108665/1‑151 (MQ=255)                                                                      gCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGt                                                                           >  2:378848/1‑151 (MQ=255)                                                                         cGCTGGGGATTTCCGTTGGCGGCAGGCCATAGCGGACAATCGGGTGATCGGCGCGGGTTTCCCGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTGCTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTgcg                                                                        <  1:967095/151‑1 (MQ=255)                                                                          gCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTgc                                                                         <  1:1145600/149‑1 (MQ=255)                                                                             gtgGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCt                                                                      <  1:506625/149‑1 (MQ=255)                                                                             gtgGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTg                                                                     <  1:1591/150‑1 (MQ=255)                                                                              tgGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGtt                                                                   >  2:239775/1‑151 (MQ=255)                                                                               ggACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGtt                                                                   >  1:784329/1‑150 (MQ=255)                                                                                gACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTcc                                                                 <  1:840420/151‑1 (MQ=255)                                                                                  cTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCa                                                                <  2:646261/150‑1 (MQ=255)                                                                                  cTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCAt                                                               >  1:146575/1‑151 (MQ=255)                                                                                   ttCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATg                                                              >  2:399108/1‑151 (MQ=255)                                                                                     ccATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGtt                                                            <  1:789842/151‑1 (MQ=255)                                                                                     ccATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGt                                                             <  2:556522/150‑1 (MQ=255)                                                                                          ggCAGCAGGCTGTGGCCGACAAACGGGTGACCGGAGGGGGTACCTGGGGCGTGTCCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAg                                                       <  1:487593/151‑1 (MQ=255)                                                                                          ggCAGCAGGCAATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGa                                                        <  2:288960/150‑1 (MQ=255)                                                                                             agcagGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGc                                                    >  2:398025/1‑151 (MQ=255)                                                                                                agGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTc                                                  >  2:501064/1‑150 (MQ=255)                                                                                                    cATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTcagcag                                             <  2:561332/151‑1 (MQ=255)                                                                                                          cGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATc                                       >  2:94633/1‑151 (MQ=255)                                                                                                                  gggTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGc                                <  1:1120441/150‑1 (MQ=255)                                                                                                                  gggTGATCGGCGCGGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCt                               <  2:1083384/151‑1 (MQ=255)                                                                                                                   ggTGATCGGCGCGGGTATCGCGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTc                              <  1:769123/151‑1 (MQ=255)                                                                                                                            cgcgGGTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTc                     >  1:443152/1‑151 (MQ=255)                                                                                                                                ggTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCacg                  >  2:1045511/1‑150 (MQ=255)                                                                                                                                 gTATCACGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCacgac                <  2:1169203/151‑1 (MQ=255)                                                                                                                                      aCGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATc           >  2:427720/1‑151 (MQ=255)                                                                                                                                       cGGGCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCt          >  2:718848/1‑151 (MQ=255)                                                                                                                                        gggCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCt          <  1:169961/150‑1 (MQ=255)                                                                                                                                         ggCGTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCtgt        <  2:580133/151‑1 (MQ=255)                                                                                                                                            gTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCTGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCtgttgt     >  1:395480/1‑151 (MQ=255)                                                                                                                                            gTGACCGCCGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCtgttg      >  1:171790/1‑150 (MQ=255)                                                                                                                                                ccgccgGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCTGTTGTGcc  >  1:1179941/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CGATCATGTTCAGCGCGGTATAGACTTCGTTGATACGCACTTTCAGACGGGAAATAACGTCGCAGCCCTGCTCGCTGTGGACTTCCATTGGCAGCAGGCCATAGCCGACAAACGGGTGATCGGCGCGGGTATCACGGGCGTGACCGCTGGCACGGACCATCGGGCCGACGTTACTGAAGTCGCGAGCGATTTCCGGGTCCAGACGACCAATGCCGACAGTGCGCTGTTCCATGTTCGGAGTGCTCAGCAGCACATCCACCAGCTCCTGCACTTCACGACGCATCTGTTGTGCC  >  NC_000913/2845136‑2845428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑