Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,196	T→C	K152K (AAA→AAG)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,196	0	T	C	100.0%	240.6 / NA	63	K152K (AAA→AAG)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/31);  total (32/31)

AGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTA  >  NC_000913/2913048‑2913340                                                                                                                                                     |                                                                                                                                                 aGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGctt                                                                                                                                                <  1:968838/151‑1 (MQ=255) aGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGctt                                                                                                                                                >  1:380044/1‑151 (MQ=255) aGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGctt                                                                                                                                                <  2:662336/151‑1 (MQ=255)        ttCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACt                                                                                                                                         >  2:45140/1‑151 (MQ=255)           cAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGTTGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACg                                                                                                                                      <  1:985392/151‑1 (MQ=255)           cAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACg                                                                                                                                      <  2:400961/151‑1 (MQ=255)            aGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACg                                                                                                                                      >  2:956619/1‑150 (MQ=255)              ttCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGc                                                                                                                                     >  1:579269/1‑149 (MQ=255)                   aTTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGaaa                                                                                                                              <  2:1075977/151‑1 (MQ=255)                     ttCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAat                                                                                                                            >  2:1026143/1‑151 (MQ=255)                       cAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatca                                                                                                                          <  1:859404/151‑1 (MQ=255)                         gTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcatc                                                                                                                        <  1:48542/151‑1 (MQ=255)                           tGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCg                                                                                                                       >  1:187609/1‑150 (MQ=255)                              ccGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCa                                                                                                                   <  2:356232/151‑1 (MQ=255)                               cGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCAt                                                                                                                  <  2:1035291/151‑1 (MQ=255)                               cGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCAt                                                                                                                  <  1:1058281/151‑1 (MQ=255)                                      gagaCGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCaa                                                                                                            >  1:671132/1‑150 (MQ=255)                                      gagaCGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAAt                                                                                                           <  1:129630/151‑1 (MQ=255)                                        gaCGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATaa                                                                                                         <  2:1092513/151‑1 (MQ=255)                                        gaCGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATaa                                                                                                         <  1:547205/151‑1 (MQ=255)                                          cGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAAc                                                                                                        >  1:407194/1‑150 (MQ=255)                                          cGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACa                                                                                                       <  1:288969/151‑1 (MQ=255)                                          cGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACa                                                                                                       <  2:746537/151‑1 (MQ=255)                                           ggTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACAt                                                                                                      >  2:223978/1‑151 (MQ=255)                                           ggTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACAt                                                                                                      <  1:855243/151‑1 (MQ=255)                                              tAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCg                                                                                                   >  1:1105962/1‑151 (MQ=255)                                                   aGCGGTGCGTAGATGTTGGTCCCCTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGaa                                                                                              <  2:506300/151‑1 (MQ=255)                                                        tGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGtt                                                                                          >  1:963463/1‑150 (MQ=255)                                                           gTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATc                                                                                       <  2:429244/150‑1 (MQ=255)                                                             aGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGa                                                                                     <  2:29404/150‑1 (MQ=255)                                                                    ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCg                                                                             <  2:312104/151‑1 (MQ=255)                                                                            ctTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGaa                                                                       <  1:1088762/149‑1 (MQ=255)                                                                            ctTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAAc                                                                     >  2:31787/1‑151 (MQ=255)                                                                             ttttGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAAc                                                                     >  2:40143/1‑150 (MQ=255)                                                                                  ccgccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATc                                                               <  2:342645/151‑1 (MQ=255)                                                                                   cgccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATc                                                               >  2:865203/1‑150 (MQ=255)                                                                                    gccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATc                                                               >  1:131993/1‑149 (MQ=255)                                                                                     ccAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAgt                                                            >  2:393575/1‑151 (MQ=255)                                                                                       aGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAgtgt                                                          <  2:21346/151‑1 (MQ=255)                                                                                          acacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACa                                                                    <  1:582876/138‑1 (MQ=255)                                                                                          acacGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACa                                                                    >  2:583018/1‑138 (MQ=255)                                                                                            acGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTc                                                     >  1:269132/1‑151 (MQ=255)                                                                                             cGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCg                                                    >  2:1019530/1‑151 (MQ=255)                                                                                                       ccGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTg                                          <  2:497316/151‑1 (MQ=255)                                                                                                           cgcATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCgg                                      <  1:265273/151‑1 (MQ=255)                                                                                                            gcATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCgg                                      >  2:111620/1‑150 (MQ=255)                                                                                                            gcATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCgg                                      >  1:591672/1‑150 (MQ=255)                                                                                                             cATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGAt                                    <  2:464900/151‑1 (MQ=255)                                                                                                              aTCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATc                                   >  2:991308/1‑151 (MQ=255)                                                                                                               tCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcg                                  >  1:25980/1‑151 (MQ=255)                                                                                                                cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgc                                 >  2:741580/1‑151 (MQ=255)                                                                                                                cTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgc                                 >  1:16606/1‑151 (MQ=255)                                                                                                                  tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgccg                               >  1:425826/1‑151 (MQ=255)                                                                                                                  tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATc                                   <  2:671288/147‑1 (MQ=255)                                                                                                                    tACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCgccgcc                             >  2:181950/1‑151 (MQ=255)                                                                                                                     aCTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCa                            <  1:817878/151‑1 (MQ=255)                                                                                                                      cTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCat                           >  2:852207/1‑151 (MQ=255)                                                                                                                           cgcAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCACCATATcac                      <  1:865330/151‑1 (MQ=255)                                                                                                                             caaaTGAGCAATATGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcac                      <  1:105427/146‑1 (MQ=255)                                                                                                                             cAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacgc                    >  2:35554/1‑151 (MQ=255)                                                                                                                                      aaTACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTg            >  1:565863/1‑150 (MQ=255)                                                                                                                                      aaTACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTg            >  2:800287/1‑150 (MQ=255)                                                                                                                                         aCGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAAt         >  1:329762/1‑150 (MQ=255)                                                                                                                                               cGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTa  <  2:1108598/151‑1 (MQ=255)                                                                                                                                               cGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCAGCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTa  <  1:1025902/151‑1 (MQ=255)                                                                                                                                                gCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTa  >  2:197227/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                 AGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTA  >  NC_000913/2913048‑2913340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑