Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,356,384	T→C	S554P (TCG→CCG)	gltB →	glutamate synthase, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,356,384	0	T	C	100.0%	224.2 / NA	59	S554P (TCG→CCG)	gltB	glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAAA  >  NC_000913/3356238‑3356529                                                                                                                                                    |                                                                                                                                                  actTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGcc                                                                                                                                               >  2:679644/1‑151 (MQ=255)   ttCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAAccgccg                                                                                                                                              >  2:262661/1‑150 (MQ=255)        ccAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTg                                                                                                                                        >  1:302932/1‑151 (MQ=255)         cagcagTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAGCCGCCGATTCTGc                                                                                                                                       >  1:75493/1‑151 (MQ=255)                   cccAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCg                                                                                                                              >  2:240234/1‑150 (MQ=255)                       gttaacaaccccGCCATCCGACCCGCTGCGTGAAGCGCATGTTATGTCGCCCGCCCCCAGTACCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGCCCGGGCGCCCCGTTT‑AAGTTTTAAACCGCCGATTCTGCTCTACTCCGAtt                                                                                                                           <  1:301730/140‑1 (MQ=255)                        gTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTc                                                                                                                         >  2:1072519/1‑150 (MQ=255)                            cTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAAc                                                                                                                     >  2:1181274/1‑150 (MQ=255)                              accccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGc                                                                                                                  <  2:990673/149‑1 (MQ=255)                                cccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCt                                                                                                                 <  1:240181/150‑1 (MQ=255)                                 ccGCCAATCGACCCGCTGCGTGAAGCGCATTTTTTGTCGCTCGCCACCAGTATCGGTGGTGAAATGAGCGTCTTTGGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAg                                                                                                                   <  1:673303/147‑1 (MQ=255)                                      aaTCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacgacg                                                                                                          <  2:369161/151‑1 (MQ=255)                                          gACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCTGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATg                                                                                                       >  1:456178/1‑150 (MQ=255)                                           aCCCGGTGCATAAAGCGCATTTTTTGACGCTCGCCCCCAGTATCGGTCGTAAATTGAACTTGTTTTGAAAAGAAGGGGGCAAGGCGC‑CCGTTTAAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATg                                                                                                       <  1:227116/149‑1 (MQ=255)                                            cccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGaa                                                                                                     >  1:1143651/1‑150 (MQ=255)                                            cccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGCCCAGGCGCACCGTTT‑AAGTTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGaa                                                                                                     <  2:650654/150‑1 (MQ=255)                                             ccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAag                                                                                                   <  1:208696/151‑1 (MQ=255)                                               gCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAag                                                                                                   >  2:893901/1‑149 (MQ=255)                                                 tGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAgagg                                                                                                >  2:919359/1‑150 (MQ=255)                                                  gCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAagag                                                                                                 <  2:472397/148‑1 (MQ=255)                                                        aGCGCATTTTATGTCGCTCGCCACCAGTAGCGGTCGTAAATTGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTAc                                                                                        <  2:1175034/151‑1 (MQ=255)                                                          cgcATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACcg                                                                                      >  2:478137/1‑151 (MQ=255)                                                                    gTCGCTCGCCCCCAGTACCGGTCGTGAAATAAACGTCTTTTGCGAAGCAGAGGCCCCGGCCCCCCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACcgc                                                                                     <  1:408018/142‑1 (MQ=255)                                                                       gctcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTg                                                                         <  2:129940/151‑1 (MQ=255)                                                                        ctcgcCACCAGTATCGGTCGTGAAATGAACTTCTTTTGCGAAGCAGAGGCCCAGGCCCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTg                                                                         <  1:163399/150‑1 (MQ=255)                                                                         tcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTgg                                                                        >  1:864428/1‑150 (MQ=255)                                                                         tcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTgg                                                                        >  2:213614/1‑150 (MQ=255)                                                                           gcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGat                                                                      >  2:273089/1‑150 (MQ=255)                                                                           gcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGa                                                                       >  1:652194/1‑149 (MQ=255)                                                                            ccaccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCTTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGat                                                                      <  2:1179173/149‑1 (MQ=255)                                                                             cacgaGTATCGGTCGAGAAAGGAACGTCTTTAGGCAGGCGTGCGGCCGGGTGCCCCTTTT‑AAGCTTGAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATCCGc                                                                           <  1:56203/143‑1 (MQ=255)                                                                             caccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATc                                                                   <  2:422151/151‑1 (MQ=255)                                                                               ccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCAc                                                                 >  2:39945/1‑151 (MQ=255)                                                                                  gTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCtt                                                              >  2:109/1‑151 (MQ=255)                                                                                       ggccGTGAAATGAACGCCTTTTGCAAAGCAGAGGGCCAGGCGCCCCTTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGAc                                                          <  2:1183569/147‑1 (MQ=255)                                                                                       ggTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCGGATACGCTGGATATCACCTTTGACg                                                         >  2:1072973/1‑151 (MQ=255)                                                                                            tGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACt                                                    >  2:488935/1‑151 (MQ=255)                                                                                              aaaTGACCGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACt                                                    <  1:98235/149‑1 (MQ=255)                                                                                              aaaTGAACGTCTTTTGCGAAGCAGAGGGCCAGGCCCCCCTTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTa                                                   <  1:594480/150‑1 (MQ=255)                                                                                                   aaCGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAAcc                                              <  1:262604/150‑1 (MQ=255)                                                                                                    aCGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCAc                                            >  1:1086931/1‑151 (MQ=255)                                                                                                          tttGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCg                                       >  1:717564/1‑150 (MQ=255)                                                                                                          tttGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATAAGCTGGATATCACCTTTGACGTCACTAAAACCACGCTc                                        <  2:303004/149‑1 (MQ=255)                                                                                                             gCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAg                                    <  1:1093288/150‑1 (MQ=255)                                                                                                              cGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCg                                  >  2:1156117/1‑151 (MQ=255)                                                                                                                aaGCAGAGGGCCGGGCGCCCCTTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTc                                        <  1:1011323/143‑1 (MQ=255)                                                                                                                 aGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGAc                                >  2:707581/1‑150 (MQ=255)                                                                                                                      agGGCCAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCa                           >  1:128579/1‑150 (MQ=255)                                                                                                                       gggCCAGGCGCCCCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGt                             <  1:1099329/147‑1 (MQ=255)                                                                                                                        ggCCAGGCGCCCCTTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAag                        <  1:679484/151‑1 (MQ=255)                                                                                                                           cAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGc                     <  1:398780/151‑1 (MQ=255)                                                                                                                           cAGGCGCACCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGc                     >  2:918727/1‑151 (MQ=255)                                                                                                                                  aCCGTTT‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCg               >  1:1065335/1‑150 (MQ=255)                                                                                                                                      ttt‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACaa           >  1:642332/1‑150 (MQ=255)                                                                                                                                        t‑AAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAg         <  1:810429/150‑1 (MQ=255)                                                                                                                                          aaGCTTTAAACCGCCGATTTTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGcc       >  2:391649/1‑151 (MQ=255)                                                                                                                                          aaGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGcc       <  2:1062415/151‑1 (MQ=255)                                                                                                                                           aGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCg      >  2:946861/1‑151 (MQ=255)                                                                                                                                               ttAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaa    <  2:85175/149‑1 (MQ=255)                                                                                                                                                 aaaCCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaaaa  <  1:1085363/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT‑AAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAAA  >  NC_000913/3356238‑3356529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑