Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,928	C→T	A54T (GCA→ACA)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,928	0	C	T	100.0%	328.1 / NA	84	A54T (GCA→ACA)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/44);  total (40/44)

AAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAA  >  NC_000913/3400780‑3401074                                                                                                                                                     |                                                                                                                                                   aaGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAg                                                                                                                                                  <  2:830386/151‑1 (MQ=255)   gCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCt                                                                                                                                                >  2:97653/1‑151 (MQ=255)     tGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTAc                                                                                                                                              >  2:263790/1‑151 (MQ=255)        tGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGc                                                                                                                                            <  2:161880/150‑1 (MQ=255)            gtgCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCttt                                                                                                                                         <  2:1146454/149‑1 (MQ=255)            gtgCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTc                                                                                                                                       <  1:747420/151‑1 (MQ=255)                aCTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGt                                                                                                                                    >  2:14228/1‑150 (MQ=255)                   tGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAAc                                                                                                                                >  2:1101421/1‑151 (MQ=255)                     tttGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCg                                                                                                                              >  2:6526/1‑151 (MQ=255)                      ttGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCgg                                                                                                                             >  1:420153/1‑151 (MQ=255)                      ttGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCg                                                                                                                              <  1:908580/150‑1 (MQ=255)                        gatgaAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGc                                                                                                                            >  2:928668/1‑150 (MQ=255)                         atgaAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCAc                                                                                                                          <  2:32069/151‑1 (MQ=255)                                gCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCt                                                                                                                    <  2:746531/150‑1 (MQ=255)                                  tGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGAc                                                                                                                 >  2:104010/1‑151 (MQ=255)                                   tgAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACg                                                                                                                >  1:121566/2‑151 (MQ=255)                                    gAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACg                                                                                                                >  2:11288/1‑150 (MQ=255)                                      gCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAAt                                                                                                             <  1:922757/151‑1 (MQ=255)                                      gCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAAt                                                                                                             <  1:483349/151‑1 (MQ=255)                                        aTTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTTGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATgg                                                                                                           >  1:910217/1‑151 (MQ=255)                                          tttttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGTATgg                                                                                                           >  1:644549/1‑149 (MQ=255)                                            tttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGccac                                                                                                       >  1:640731/1‑151 (MQ=255)                                            tttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGcca                                                                                                        <  2:486771/150‑1 (MQ=255)                                              tCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGccacc                                                                                                      >  1:659790/1‑150 (MQ=255)                                               cAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCcaccac                                                                                                    >  1:917068/1‑151 (MQ=255)                                                 gTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACgg                                                                                                  <  2:491990/151‑1 (MQ=255)                                                   cACGAAGAAGGCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGa                                                                                                 <  1:164472/150‑1 (MQ=255)                                                    aCGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGa                                                                                                 >  2:88518/1‑149 (MQ=255)                                                              cGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCa                                                                                      >  2:907992/1‑150 (MQ=255)                                                                 cGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCa                                                                                      >  2:1029171/1‑147 (MQ=255)                                                                 cGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCa                                                                                      <  1:1028929/147‑1 (MQ=255)                                                                     aaCGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATg                                                                              >  2:235872/1‑151 (MQ=255)                                                                     aaCGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATg                                                                              >  2:1047139/1‑151 (MQ=255)                                                                     aaCGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATg                                                                              >  1:5669/1‑151 (MQ=255)                                                                          cGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTg                                                                         <  1:704643/151‑1 (MQ=255)                                                                          cGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTg                                                                         >  2:211331/1‑151 (MQ=255)                                                                           gTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGt                                                                        <  1:88494/151‑1 (MQ=255)                                                                             cTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTcc                                                                      >  1:709786/1‑151 (MQ=255)                                                                             cTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTcc                                                                      >  2:463213/1‑151 (MQ=255)                                                                             cTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTcc                                                                      >  1:813982/1‑151 (MQ=255)                                                                               ttCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCGCGATCCTCACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTcc                                                                      <  1:1027087/149‑1 (MQ=255)                                                                               ttCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTc                                                                       <  1:527933/148‑1 (MQ=255)                                                                               ttCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCt                                                                     <  2:1186198/150‑1 (MQ=255)                                                                                tCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCttt                                                                   >  1:358342/1‑151 (MQ=255)                                                                                 cATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCtttt                                                                  <  2:213570/151‑1 (MQ=255)                                                                                 cATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCttt                                                                   <  2:1108697/150‑1 (MQ=255)                                                                                   ttGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTa                                                                 >  1:620035/1‑150 (MQ=255)                                                                                    tGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTAc                                                                >  1:789734/1‑150 (MQ=255)                                                                                     gggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACa                                                               <  1:463101/150‑1 (MQ=255)                                                                                      ggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATa                                                             <  1:589377/151‑1 (MQ=255)                                                                                        cGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATaaa                                                           <  1:820296/151‑1 (MQ=255)                                                                                           aTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATg                                                         >  1:279922/1‑150 (MQ=255)                                                                                             ggCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAgg                                                      <  1:1007593/151‑1 (MQ=255)                                                                                              gcagcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAggg                                                     <  2:16462/151‑1 (MQ=255)                                                                                               cagcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGt                                                    >  2:925435/1‑151 (MQ=255)                                                                                                  caATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAtt                                                 <  1:76763/151‑1 (MQ=255)                                                                                                  caATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAt                                                  <  2:375017/150‑1 (MQ=255)                                                                                                   aaTATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTc                                                <  2:1019636/151‑1 (MQ=255)                                                                                                    atatTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCg                                               <  1:1051476/151‑1 (MQ=255)                                                                                                    atatTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCg                                               >  2:169309/1‑151 (MQ=255)                                                                                                        tGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCACTACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGt                                           >  1:221232/1‑151 (MQ=255)                                                                                                        tGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGt                                           >  1:813603/1‑151 (MQ=255)                                                                                                          cccGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAc                                         <  1:916937/151‑1 (MQ=255)                                                                                                            cGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAccc                                       <  1:1086008/151‑1 (MQ=255)                                                                                                              gCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAccc                                       >  1:771522/1‑149 (MQ=255)                                                                                                              gCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAg                                     <  2:908359/151‑1 (MQ=255)                                                                                                                gTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGt                                   >  1:874578/1‑151 (MQ=255)                                                                                                                gTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGt                                   <  1:337354/151‑1 (MQ=255)                                                                                                                gTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGt                                   <  1:1021048/151‑1 (MQ=255)                                                                                                                  aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCa                                 <  2:712051/151‑1 (MQ=255)                                                                                                                      cccAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATgg                             <  1:1110719/151‑1 (MQ=255)                                                                                                                         aGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGAc                           >  2:574395/1‑150 (MQ=255)                                                                                                                          gCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACaa                         <  2:186001/151‑1 (MQ=255)                                                                                                                           cATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAg                        <  2:651774/151‑1 (MQ=255)                                                                                                                              cTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTc                      >  2:649943/1‑150 (MQ=255)                                                                                                                                gCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTc                      <  2:1039760/148‑1 (MQ=255)                                                                                                                                gCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAtt                   >  1:528594/1‑151 (MQ=255)                                                                                                                                       gTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAAc            <  1:11289/151‑1 (MQ=255)                                                                                                                                          aTGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATg         <  1:721788/151‑1 (MQ=255)                                                                                                                                               cTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg    >  1:573449/1‑151 (MQ=255)                                                                                                                                                tACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACg    >  2:651456/1‑150 (MQ=255)                                                                                                                                                 aCTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGaa  <  1:670459/151‑1 (MQ=255)                                                                                                                                                 aCTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGaa  <  1:280247/151‑1 (MQ=255)                                                                                                                                                 aCTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGaa  <  1:367474/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAA  >  NC_000913/3400780‑3401074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑