Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,439,982 A→G S6S (AGT→AGC)  rplQ ← 50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,9820AG98.4% 235.9 / ‑3.7 64S6S (AGT→AGC) rplQ50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (29/34);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

GCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAG  >  NC_000913/3439836‑3440128
                                                                                                                                                  |                                                                                                                                                  
gCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGctct                                                                                                                                                <  1:1174880/151‑1 (MQ=255)
  aaGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCtt                                                                                                                                               >  1:998692/1‑150 (MQ=255)
    gagTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACg                                                                                                                                            <  2:743659/151‑1 (MQ=255)
     agTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGa                                                                                                                                           <  2:1085299/151‑1 (MQ=255)
      gTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGAt                                                                                                                                          >  2:705020/1‑151 (MQ=255)
        aaTCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGAt                                                                                                                                          <  1:1019273/149‑1 (MQ=255)
        aaTCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATgg                                                                                                                                        <  2:581305/151‑1 (MQ=255)
           cAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGgcgc                                                                                                                                     >  2:626709/1‑151 (MQ=255)
           cAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGgcg                                                                                                                                      <  2:748374/150‑1 (MQ=255)
            aGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCa                                                                                                                                    <  2:501950/151‑1 (MQ=255)
                gCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGAc                                                                                                                                >  1:387910/1‑151 (MQ=255)
                gCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGAc                                                                                                                                >  2:1145415/1‑151 (MQ=255)
                  tCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCt                                                                                                                              >  1:37037/1‑151 (MQ=255)
                   cAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGAcc                                                                                                                               <  2:684458/149‑1 (MQ=255)
                   cAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCtt                                                                                                                             >  2:884475/1‑151 (MQ=255)
                      cTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTaac                                                                                                                          >  1:300203/1‑149 (MQ=255)
                         cgcgGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCtt                                                                                                                       <  2:542352/151‑1 (MQ=255)
                            ggcgCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTtctc                                                                                                                    <  1:617157/151‑1 (MQ=255)
                              cgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCa                                                                                                                   <  1:281708/150‑1 (MQ=255)
                               gcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAg                                                                                                                  <  1:824990/150‑1 (MQ=255)
                                     ctTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTaaaa                                                                                                             <  2:220459/149‑1 (MQ=255)
                                          gCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAc                                                                                                      >  2:1098270/1‑151 (MQ=255)
                                             ttAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAcc                                                                                                     <  2:695554/149‑1 (MQ=255)
                                               aGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCtgtg                                                                                                 >  2:970986/1‑151 (MQ=255)
                                                      gtcgtcTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGt                                                                                          >  2:345786/1‑151 (MQ=255)
                                                        cgtcTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGtt                                                                                         >  2:40563/1‑150 (MQ=255)
                                                         gtcTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTAc                                                                                       >  2:919444/1‑151 (MQ=255)
                                                         gtcTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTAc                                                                                       >  2:745543/1‑151 (MQ=255)
                                                              gatgatTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTc                                                                                  >  1:282201/1‑151 (MQ=255)
                                                                     tCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGAt                                                                            <  2:773751/150‑1 (MQ=255)
                                                                       aTGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCt                                                                         >  1:1063075/1‑151 (MQ=255)
                                                                         gACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTg                                                                       <  2:1000272/151‑1 (MQ=255)
                                                                            gAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCg                                                                    >  2:512820/1‑151 (MQ=255)
                                                                                   tGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCATTCTCAGTAAAACCTTAACATGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTg                                                                 >  1:1152996/1‑147 (MQ=255)
                                                                                    gAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGcc                                                              >  2:168107/1‑149 (MQ=255)
                                                                                        cTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGtttt                                                        >  1:245566/1‑151 (MQ=255)
                                                                                        cTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGtttt                                                        >  1:71808/1‑151 (MQ=255)
                                                                                          gCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTcc                                                      <  2:209237/151‑1 (MQ=255)
                                                                                            cATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAg                                                    <  1:1050297/151‑1 (MQ=255)
                                                                                               atTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGgcg                                                 <  2:282271/151‑1 (MQ=255)
                                                                                               atTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGgcg                                                 <  2:5555/151‑1 (MQ=255)
                                                                                                         aTAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCaga                                       <  1:293272/151‑1 (MQ=255)
                                                                                                         aTAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCaga                                       <  2:1090588/151‑1 (MQ=255)
                                                                                                           aGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAgaga                                     <  2:218691/151‑1 (MQ=255)
                                                                                                             ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACa                                   <  2:620070/151‑1 (MQ=255)
                                                                                                             ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGAc                                    >  2:1075542/1‑150 (MQ=255)
                                                                                                               tGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGt                                 >  2:376131/1‑151 (MQ=255)
                                                                                                                ggCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGt                                 <  1:900867/150‑1 (MQ=255)
                                                                                                                 gCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTcc                               >  2:1118485/1‑151 (MQ=255)
                                                                                                                   gATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCAc                             >  2:167669/1‑151 (MQ=255)
                                                                                                                       gctgctGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGa                         <  1:593884/151‑1 (MQ=255)
                                                                                                                            tgTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGcc                     >  1:931701/1‑150 (MQ=255)
                                                                                                                             gTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAg                   <  1:731050/151‑1 (MQ=255)
                                                                                                                               tGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGc                  <  2:361140/150‑1 (MQ=255)
                                                                                                                                  ggTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGt              <  2:156261/151‑1 (MQ=255)
                                                                                                                                   gTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTc             <  1:548121/151‑1 (MQ=255)
                                                                                                                                   gTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTc             >  1:853921/1‑151 (MQ=255)
                                                                                                                                      cAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCtt           <  1:1147321/150‑1 (MQ=255)
                                                                                                                                       aGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCttt          >  2:793772/1‑150 (MQ=255)
                                                                                                                                        gTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTa         >  2:220868/1‑150 (MQ=255)
                                                                                                                                         ttGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAt       >  2:392550/1‑151 (MQ=255)
                                                                                                                                           gacgacCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtct     <  1:75525/151‑1 (MQ=255)
                                                                                                                                             cgacCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa   <  2:758886/151‑1 (MQ=255)
                                                                                                                                              gacCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAg  <  1:970753/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAG  >  NC_000913/3439836‑3440128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑