Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,462,301	A→G	T110A (ACG→GCG)	gspI →	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,462,301	0	A	G	100.0%	316.7 / NA	86	T110A (ACG→GCG)	gspI	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/39);  total (47/39)

GAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTC  >  NC_000913/3462157‑3462447                                                                                                                                                 |                                                                                                                                                   gAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGtt                                                                                                                                              >  1:719013/1‑151 (MQ=255) gAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGtt                                                                                                                                              <  2:235673/151‑1 (MQ=255) gAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGt                                                                                                                                               <  2:1173785/150‑1 (MQ=255)   ggAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTAt                                                                                                                                            <  1:597895/151‑1 (MQ=255)     aaaaCACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATc                                                                                                                                           >  2:1051315/1‑150 (MQ=255)     aaaaCACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCa                                                                                                                                          <  1:450484/151‑1 (MQ=255)      aaaCACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCa                                                                                                                                          >  1:147306/1‑150 (MQ=255)        acacGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCaa                                                                                                                                         >  2:494633/1‑149 (MQ=255)          acGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAg                                                                                                                                       >  2:810603/1‑149 (MQ=255)          acGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGc                                                                                                                                      >  2:286793/1‑150 (MQ=255)          acGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCa                                                                                                                                     <  1:796060/151‑1 (MQ=255)              ccAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGa                                                                                                                                 <  2:1046625/151‑1 (MQ=255)               cAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGat                                                                                                                                >  2:268060/1‑151 (MQ=255)                 gTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGata                                                                                                                               >  1:94243/1‑150 (MQ=255)                    cTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataata                                                                                                                            >  1:875762/1‑150 (MQ=255)                       gCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATc                                                                                                                        >  2:943777/1‑151 (MQ=255)                        cAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGAGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATcc                                                                                                                       >  1:45960/1‑151 (MQ=255)                         aaaGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCg                                                                                                                      >  1:298063/1‑151 (MQ=255)                         aaaGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCg                                                                                                                      >  1:1149911/1‑151 (MQ=255)                                  ataaATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcac                                                                                                             <  2:183548/151‑1 (MQ=255)                                  ataaATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcac                                                                                                             >  2:688269/1‑151 (MQ=255)                                    aaaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                            >  2:951863/1‑150 (MQ=255)                                    aaaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACaa                                                                                                           <  2:343551/151‑1 (MQ=255)                                    aaaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACaa                                                                                                           <  1:246318/151‑1 (MQ=255)                                    aaaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACACTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                            >  2:896172/1‑150 (MQ=255)                                      aTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                            >  1:438539/1‑148 (MQ=255)                                         gCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAgatga                                                                                                      <  1:885115/151‑1 (MQ=255)                                           gAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGAtt                                                                                                    >  1:505508/1‑151 (MQ=255)                                           gAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGAtt                                                                                                    >  2:905796/1‑151 (MQ=255)                                             agagTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTa                                                                                                   >  2:1118072/1‑150 (MQ=255)                                               agTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATc                                                                                                >  2:141082/1‑151 (MQ=255)                                               agTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCAGGCAGGCACAAGATGATTAATc                                                                                                <  2:134898/151‑1 (MQ=255)                                                   gAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCca                                                                                            <  1:416663/151‑1 (MQ=255)                                                     aCTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTATCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGAAGGCACAAGATGATTAATCGCcag                                                                                           >  1:810172/1‑150 (MQ=255)                                                      cTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcagc                                                                                          >  2:600941/1‑150 (MQ=255)                                                       tGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcagc                                                                                          >  1:141733/1‑149 (MQ=255)                                                       tGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcagc                                                                                          >  1:956631/1‑149 (MQ=255)                                                       tGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcagc                                                                                          >  2:915492/1‑149 (MQ=255)                                                             aGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTc                                                                                  >  2:208212/1‑151 (MQ=255)                                                                  tatCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCGGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACAtt                                                                             >  1:674298/1‑151 (MQ=255)                                                                   atCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACAtt                                                                             <  1:133038/150‑1 (MQ=255)                                                                   atCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTg                                                                            >  2:506702/1‑151 (MQ=255)                                                                   atCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTg                                                                            >  1:571642/1‑151 (MQ=255)                                                                          cAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAg                                                                      <  1:914839/150‑1 (MQ=255)                                                                            aGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTc                                                                   <  1:875597/151‑1 (MQ=255)                                                                              cAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCAt                                                                 >  2:1052042/1‑151 (MQ=255)                                                                                aaGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATgg                                                               <  2:547285/151‑1 (MQ=255)                                                                                  gACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggcg                                                             <  2:520898/151‑1 (MQ=255)                                                                                  gACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggcg                                                             <  1:333602/151‑1 (MQ=255)                                                                                    cGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggcggc                                                           >  1:851327/1‑151 (MQ=255)                                                                                    cGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggcgg                                                            >  1:648300/1‑150 (MQ=255)                                                                                          gCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGc                                                     <  2:298246/151‑1 (MQ=255)                                                                                            ttCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCg                                                    >  2:593554/1‑150 (MQ=255)                                                                                             tCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCg                                                    <  2:45971/149‑1 (MQ=255)                                                                                                  aaCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGAttt                                                <  2:1024616/148‑1 (MQ=255)                                                                                                  aaCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtct                                             <  2:349070/151‑1 (MQ=255)                                                                                                  aaCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtct                                             >  1:196941/1‑151 (MQ=255)                                                                                                      cACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCaa                                          <  2:358847/150‑1 (MQ=255)                                                                                                         cATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCt                                      >  2:991502/1‑151 (MQ=255)                                                                                                            aaCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTa                                    <  1:866901/150‑1 (MQ=255)                                                                                                             aCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGc                                  <  2:543262/151‑1 (MQ=255)                                                                                                              cGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCg                                 >  2:904058/1‑151 (MQ=255)                                                                                                              cGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCg                                 >  1:919210/1‑151 (MQ=255)                                                                                                                  gACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGc                              <  2:1150171/150‑1 (MQ=255)                                                                                                                   aCATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                            >  1:1000210/1‑151 (MQ=255)                                                                                                                   aCATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                            >  2:250280/1‑151 (MQ=255)                                                                                                                   aCATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                            <  1:1051067/151‑1 (MQ=255)                                                                                                                   aCATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                            <  1:1073460/151‑1 (MQ=255)                                                                                                                   aCATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                            <  2:142372/151‑1 (MQ=255)                                                                                                                     aTTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTgg                           >  1:214022/1‑150 (MQ=255)                                                                                                                        aCCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                       <  2:147342/151‑1 (MQ=255)                                                                                                                          ccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                      >  1:570895/1‑150 (MQ=255)                                                                                                                          ccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTa                     <  2:887296/151‑1 (MQ=255)                                                                                                                          ccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTa                     <  2:1034096/151‑1 (MQ=255)                                                                                                                             gCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGa                  <  2:183917/151‑1 (MQ=255)                                                                                                                              cGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGGTTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAt                 >  1:643909/1‑151 (MQ=255)                                                                                                                                  cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtttt              <  2:382590/150‑1 (MQ=255)                                                                                                                                   aGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtttttt            >  2:832997/1‑151 (MQ=255)                                                                                                                                     aCAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCg          <  2:607253/151‑1 (MQ=255)                                                                                                                                     aCAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCg          <  2:1104650/151‑1 (MQ=255)                                                                                                                                         ccTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCa        >  1:966334/1‑149 (MQ=255)                                                                                                                                            cGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCtt   <  1:661944/151‑1 (MQ=255)                                                                                                                                            cGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCt    <  2:505634/150‑1 (MQ=255)                                                                                                                                             gCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCt                             >  1:504792/1‑124 (MQ=255)                                                                                                                                             gCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCt                             <  2:504918/124‑1 (MQ=255)                                                                                                                                             gCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTc  >  1:1082595/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTC  >  NC_000913/3462157‑3462447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑