Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,190	A→G	Y24C (TAC→TGC)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,190	0	A	G	100.0%	286.7 / NA	73	Y24C (TAC→TGC)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/36);  total (37/36)

AGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGC  >  NC_000913/3486046‑3486335                                                                                                                                                 |                                                                                                                                                  aGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATc                                                                                                                                             >  1:91827/1‑151 (MQ=255)   cATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATcc                                                                                                                                            <  2:798575/150‑1 (MQ=255)         tCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGc                                                                                                                                      <  2:950521/150‑1 (MQ=255)              aaTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCtt                                                                                                                                >  2:392655/1‑151 (MQ=255)                tCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCt                                                                                                                                 <  1:845026/148‑1 (MQ=255)                  cAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTc                                                                                                                            <  2:978530/151‑1 (MQ=255)                         aGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGTTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAg                                                                                                                       >  2:129314/1‑149 (MQ=255)                         aGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCa                                                                                                                        <  1:660427/148‑1 (MQ=255)                          gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAggg                                                                                                                     >  1:307429/1‑150 (MQ=255)                          gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGt                                                                                                                    <  2:787/151‑1 (MQ=255)                            ggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTg                                                                                                                   >  1:778325/1‑150 (MQ=255)                             gcgTTATCTGGCTCTGGAGAAAGCTTATAAAAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGACTGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTg                                                                                                                   >  1:1165000/1‑149 (MQ=255)                               gTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                                                                                                >  2:341545/1‑150 (MQ=255)                                ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                               >  2:1142437/1‑150 (MQ=255)                                 tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                               >  1:219096/1‑149 (MQ=255)                                  aTCTGGCTCTGGAGAAAGCTTATACCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAg                                                                                                             <  1:1022460/150‑1 (MQ=255)                                      ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGa                                                                                                         <  1:1106717/150‑1 (MQ=255)                                       gCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                        >  2:1121774/1‑150 (MQ=255)                                        ctctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                                                                                      >  2:3506/1‑151 (MQ=255)                                        ctctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                                                                                      >  2:1085576/1‑151 (MQ=255)                                          ctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                                                                                      <  1:5377/149‑1 (MQ=255)                                            ggAGAAAGCTTATACCAGAGGATAACCGGGTATGGTGCTTGGCAACCCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg                                                                                                  <  2:941323/151‑1 (MQ=255)                                            ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                                                                                   <  1:34391/150‑1 (MQ=255)                                            ggAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCt                                                                                                   <  2:164645/150‑1 (MQ=255)                                               gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtac                                                                                               >  2:654829/1‑151 (MQ=255)                                                aaaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtact                                                                                              <  2:241659/151‑1 (MQ=255)                                                aaaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtact                                                                                              >  2:214639/1‑151 (MQ=255)                                                       ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                                                                                        <  2:700826/150‑1 (MQ=255)                                                       ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                                                                                       <  2:809427/151‑1 (MQ=255)                                                       ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGt                                                                                       >  1:234335/1‑151 (MQ=255)                                                         aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                      >  1:509843/1‑150 (MQ=255)                                                         aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                     <  2:408828/151‑1 (MQ=255)                                                         aaCAGAGGATAACCGCGCATGGTGCTTGGAAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                      <  1:1047976/150‑1 (MQ=255)                                                          aCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                     >  1:583515/1‑150 (MQ=255)                                                           cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa                                                                                   <  1:723602/151‑1 (MQ=255)                                                            agagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa                                                                                    >  1:988768/1‑149 (MQ=255)                                                            agagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAg                                                                                  <  1:622569/151‑1 (MQ=255)                                                             gagGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAgg                                                                                 >  1:260386/1‑151 (MQ=255)                                                                 aTAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct                                                                             >  2:530688/1‑151 (MQ=255)                                                                          cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg                                                                    <  2:75376/151‑1 (MQ=255)                                                                          cATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg                                                                    >  2:620774/1‑151 (MQ=255)                                                                                 cTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATc                                                              >  1:322298/1‑150 (MQ=255)                                                                                     gCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGa                                                         <  2:422344/151‑1 (MQ=255)                                                                                      caaacagcaaacagACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGAc                                                        >  1:112982/1‑151 (MQ=255)                                                                                       aaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGAc                                                        <  1:530555/150‑1 (MQ=255)                                                                                                  aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaa                                             >  1:589192/1‑150 (MQ=255)                                                                                                     cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGa                                          >  1:832774/1‑150 (MQ=255)                                                                                                      ccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGaa                                         >  1:1143770/1‑150 (MQ=255)                                                                                                       cGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAAt                                       <  2:249682/151‑1 (MQ=255)                                                                                                         aCTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATg                                      >  1:244415/1‑150 (MQ=255)                                                                                                         aCTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGa                                     <  2:765055/151‑1 (MQ=255)                                                                                                           tctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                   >  1:810547/1‑151 (MQ=255)                                                                                                             tcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCct                                 >  2:661366/1‑151 (MQ=255)                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                  <  2:985931/149‑1 (MQ=255)                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                  <  2:501179/149‑1 (MQ=255)                                                                                                                  tGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCt                            >  1:1025515/1‑151 (MQ=255)                                                                                                                    gTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTAt                          <  2:62493/151‑1 (MQ=255)                                                                                                                      tCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                        >  2:277395/1‑151 (MQ=255)                                                                                                                      tCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                        >  1:294908/1‑151 (MQ=255)                                                                                                                         tGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                        <  1:926439/148‑1 (MQ=255)                                                                                                                         tGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTg                       <  1:263869/149‑1 (MQ=255)                                                                                                                          gTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAAt                    <  2:148281/151‑1 (MQ=255)                                                                                                                            ctcATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATc                   >  2:734713/1‑150 (MQ=255)                                                                                                                             tcATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATc                   >  2:1024601/1‑149 (MQ=255)                                                                                                                             tcATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATc                   >  2:927275/1‑149 (MQ=255)                                                                                                                              cATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATc                   <  2:1155594/148‑1 (MQ=255)                                                                                                                              cATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCa                  <  1:323112/149‑1 (MQ=255)                                                                                                                               aTTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAggg               <  2:785161/151‑1 (MQ=255)                                                                                                                                   ccACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAt           <  2:240718/151‑1 (MQ=255)                                                                                                                                      caTTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAtttt        >  1:620644/1‑151 (MQ=255)                                                                                                                                         tCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAt      <  1:3506/150‑1 (MQ=255)                                                                                                                                            tAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc  >  2:237246/1‑151 (MQ=255)                                                                                                                                             aaGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc  <  2:967378/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGC  >  NC_000913/3486046‑3486335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑