Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,531,670	A→G	L257P (CTG→CCG)	yhgE ←	DUF4153 family putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,531,670	0	A	G	100.0%	306.7 / NA	84	L257P (CTG→CCG)	yhgE	DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/37);  total (47/37)

AGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGT  >  NC_000913/3531523‑3531817                                                                                                                                                    |                                                                                                                                                    aGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCg                                                                                                                                                  >  1:1009109/1‑151 (MQ=255)  gCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCg                                                                                                                                                  >  1:59977/1‑150 (MQ=255)  gCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGt                                                                                                                                                 <  2:431956/151‑1 (MQ=255)  gCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGt                                                                                                                                                 <  2:551987/151‑1 (MQ=255)   ccATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTc                                                                                                                                                <  2:524297/151‑1 (MQ=255)       aaCGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAAc                                                                                                                                             >  2:948398/1‑150 (MQ=255)        aCGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAAc                                                                                                                                             >  2:171269/1‑149 (MQ=255)         cGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGc                                                                                                                                          <  1:483615/151‑1 (MQ=255)              aGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAAccc                                                                                                                                     >  2:470837/1‑151 (MQ=255)                gcggcgACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCcgg                                                                                                                                   <  2:92401/151‑1 (MQ=255)                gcggcgACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCcgg                                                                                                                                   >  2:308106/1‑151 (MQ=255)                gcggcgACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCcgg                                                                                                                                   <  1:720997/151‑1 (MQ=255)                  ggcgACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCcggcg                                                                                                                                 <  2:616579/151‑1 (MQ=255)                    cgacgaACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCcggcggc                                                                                                                               >  1:704366/1‑151 (MQ=255)                    cgacgaACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCcggcggc                                                                                                                               >  1:335910/1‑151 (MQ=255)                    cgacgaACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCcggcgg                                                                                                                                >  1:800691/1‑150 (MQ=255)                                 cAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCg                                                                                                                   >  1:219907/1‑150 (MQ=255)                                      gAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAAt                                                                                                             <  1:1096606/151‑1 (MQ=255)                                       aGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATc                                                                                                            >  2:18634/1‑151 (MQ=255)                                        gCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATcg                                                                                                           <  2:192863/151‑1 (MQ=255)                                         cAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATcgc                                                                                                          >  1:1107434/1‑151 (MQ=255)                                         cAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATcgc                                                                                                          <  1:1102929/151‑1 (MQ=255)                                          aaCCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATcgcg                                                                                                         >  2:316395/1‑151 (MQ=255)                                             cAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCta                                                                                                      <  1:486671/151‑2 (MQ=255)                                             cAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTc                                                                                                      >  2:1092304/1‑151 (MQ=255)                                               gCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTc                                                                                                      >  1:1088049/1‑149 (MQ=255)                                                cAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAgg                                                                                                   <  1:33025/151‑1 (MQ=255)                                                  aaGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGcc                                                                                                 <  1:733019/151‑1 (MQ=255)                                                    gCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCg                                                                                               >  2:292540/1‑151 (MQ=255)                                                                 ggCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAggg                                                                                   <  2:335989/150‑1 (MQ=255)                                                                    aacgcaacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGtgat                                                                               <  2:386591/151‑1 (MQ=255)                                                                       gcaacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTgatgat                                                                            >  2:867126/1‑151 (MQ=255)                                                                         aacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCTAAAATGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATa                                                                           >  1:461749/1‑150 (MQ=255)                                                                         aacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATaa                                                                          <  2:104000/151‑1 (MQ=255)                                                                          acgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATaa                                                                          >  2:123515/1‑150 (MQ=255)                                                                           cgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAAc                                                                        >  1:46205/1‑151 (MQ=255)                                                                           cgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAAc                                                                        <  2:1037818/151‑1 (MQ=255)                                                                            ggCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAAc                                                                        >  2:749292/1‑150 (MQ=255)                                                                              cccTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATc                                                                     >  1:1072418/1‑151 (MQ=255)                                                                                  gTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAgg                                                                  <  1:749123/150‑1 (MQ=255)                                                                                  gTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAg                                                                   <  1:491927/149‑1 (MQ=255)                                                                                     cAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTa                                                              <  2:25094/151‑1 (MQ=255)                                                                                      aGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGtt                                                               >  1:968676/1‑149 (MQ=255)                                                                                       gggAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGc                                                            >  1:262362/1‑151 (MQ=255)                                                                                       gggAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGc                                                            <  2:259835/151‑1 (MQ=255)                                                                                        ggAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGc                                                            >  1:113021/1‑150 (MQ=255)                                                                                          aaGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAAt                                                         <  1:866926/151‑1 (MQ=255)                                                                                           aGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATg                                                        >  2:583570/1‑151 (MQ=255)                                                                                           aGTGACGCTTTTTGCGGATCGCGGACAATAACCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCGCGTAAACGGCAGGGGGATGATAAAAATCAGGGGTAg                                                             >  1:667758/1‑146 (MQ=255)                                                                                            gTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGa                                                       <  2:1119834/151‑1 (MQ=255)                                                                                               aCGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATAc                                                    >  1:376266/1‑151 (MQ=255)                                                                                                cGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACt                                                   <  1:536433/151‑1 (MQ=255)                                                                                                 gCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACt                                                   >  1:487111/1‑150 (MQ=255)                                                                                                      ttGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACgg                                              <  2:167868/150‑1 (MQ=255)                                                                                                      ttGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGgc                                             >  1:425027/1‑151 (MQ=255)                                                                                                        gCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGgc                                             >  2:1109370/1‑149 (MQ=255)                                                                                                         cGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGgcagc                                          >  2:479075/1‑151 (MQ=255)                                                                                                           gATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGgcagca                                         >  2:749476/1‑150 (MQ=255)                                                                                                            aTCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAAc                                       <  2:704537/151‑1 (MQ=255)                                                                                                              cgcgGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAAccc                                     >  2:285526/1‑151 (MQ=255)                                                                                                               gcgGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAAcccc                                    >  1:899283/1‑151 (MQ=255)                                                                                                               gcgGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAAcccc                                    >  1:1160745/1‑151 (MQ=255)                                                                                                               gcgGACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAAcccc                                    >  2:244037/1‑151 (MQ=255)                                                                                                                 ggACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGt                                  <  2:247808/151‑1 (MQ=255)                                                                                                                  gACAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTg                                 <  2:944414/151‑1 (MQ=255)                                                                                                                   aCAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTgg                                <  1:631188/151‑1 (MQ=255)                                                                                                                   aCAATAGCCATTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTgg                                >  2:208710/1‑151 (MQ=255)                                                                                                                      aTAGCCATTAAGACCAAGTGCACAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCg                              <  1:175394/150‑1 (MQ=255)                                                                                                                            aTTAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACg                       <  1:316320/151‑1 (MQ=255)                                                                                                                              tAAGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGt                      <  2:2160/150‑1 (MQ=255)                                                                                                                               aaGATCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGt                      <  1:333719/149‑1 (MQ=255)                                                                                                                                  aTCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGaaca                 <  1:752267/151‑1 (MQ=255)                                                                                                                                  aTCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGaac                  >  2:228619/1‑150 (MQ=255)                                                                                                                                  aTCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGaac                  >  1:216927/1‑150 (MQ=255)                                                                                                                                  aTCAATTGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGaac                  >  2:116488/1‑150 (MQ=255)                                                                                                                                       ttGCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACttt            >  2:1003982/1‑151 (MQ=255)                                                                                                                                         gCACAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGCGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACtttt           >  1:296672/1‑150 (MQ=255)                                                                                                                                         gCAAAAAGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTg          >  1:290461/1‑151 (MQ=255)                                                                                                                                            aaaaGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGGGAACAACTTTTg          >  1:691298/1‑148 (MQ=255)                                                                                                                                             aaaGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAAt       >  2:773142/1‑150 (MQ=255)                                                                                                                                             aaaGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATa      <  1:381734/151‑1 (MQ=255)                                                                                                                                              aaGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATag     <  2:1031754/151‑1 (MQ=255)                                                                                                                                               aGGCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGACGATTAACGTGAACAACTTTTGAAt       >  2:349702/1‑148 (MQ=255)                                                                                                                                                 gCCGGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGt  <  2:652505/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGATAAACATCAGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGT  >  NC_000913/3531523‑3531817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑