Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,606,883	G→A	E145K (GAG→AAG)	zntA →	zinc, cobalt and lead efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,606,883	0	G	A	100.0%	294.5 / NA	77	E145K (GAG→AAG)	zntA	zinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/34);  total (43/34)

ATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATT  >  NC_000913/3606736‑3607026                                                                                                                                                    |                                                                                                                                                aTTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGc                                                                                                                                              >  1:703907/1‑151 (MQ=255)     gTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGtt                                                                                                                                          >  2:529558/1‑151 (MQ=255)       gCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCa                                                                                                                                        <  2:1152605/151‑1 (MQ=255)        cacaAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCaa                                                                                                                                       <  1:606386/151‑1 (MQ=255)          caAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAatc                                                                                                                                     <  2:178410/151‑1 (MQ=255)          caAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAat                                                                                                                                      <  1:280589/150‑1 (MQ=255)           aaGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAatc                                                                                                                                     >  1:568679/1‑150 (MQ=255)              ttGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATcc                                                                                                                                 >  2:937535/1‑151 (MQ=255)              ttGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATcc                                                                                                                                 >  1:596100/1‑151 (MQ=255)                 aaTCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGtt                                                                                                                              >  2:612772/1‑151 (MQ=255)                   tCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCg                                                                                                                            >  2:731971/1‑151 (MQ=255)                      gcgcTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGc                                                                                                                         <  1:461072/151‑1 (MQ=255)                       cgcTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCa                                                                                                                        <  1:1053293/151‑1 (MQ=255)                                  gCAGGCTATTCCCTGCGCGATTAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGttt                                                                                                              >  1:2380/1‑150 (MQ=255)                                   cAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTAt                                                                                                            <  2:238632/151‑1 (MQ=255)                                    aGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATc                                                                                                           >  2:997142/1‑151 (MQ=255)                                    aGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATc                                                                                                           >  2:932751/1‑151 (MQ=255)                                      gCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATcgc                                                                                                         <  1:195381/151‑1 (MQ=255)                                        tATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATcgcg                                                                                                        >  2:525692/1‑150 (MQ=255)                                          ttCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGAcc                                                                                                     >  1:646099/1‑151 (MQ=255)                                            cccTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCAc                                                                                                   >  2:837975/1‑151 (MQ=255)                                            cccTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCAc                                                                                                   >  2:961476/1‑151 (MQ=255)                                             ccTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACg                                                                                                  >  2:12239/1‑151 (MQ=255)                                              cTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGc                                                                                                 >  1:661232/1‑151 (MQ=255)                                               tGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCt                                                                                                >  1:940506/1‑151 (MQ=255)                                                  gcgATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGt                                                                                             >  2:1083184/1‑151 (MQ=255)                                                    gATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTg                                                                                           >  2:613250/1‑151 (MQ=255)                                                    gATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTg                                                                                           >  2:1090143/1‑151 (MQ=255)                                                    gATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTg                                                                                           >  1:148593/1‑151 (MQ=255)                                                       gAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTggg                                                                                         <  2:233160/150‑1 (MQ=255)                                                            ggCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTAc                                                                                   <  1:512087/151‑1 (MQ=255)                                                            ggCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTAc                                                                                   <  2:254110/151‑1 (MQ=255)                                                                gccgAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCg                                                                                >  1:45265/1‑150 (MQ=255)                                                                 ccgAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGAt                                                                              <  2:899269/151‑1 (MQ=255)                                                                  cgAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGAtt                                                                             <  2:1182498/151‑1 (MQ=255)                                                                       aaCCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTc                                                                         <  1:81611/150‑1 (MQ=255)                                                                           gCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTc                                                                      >  1:370019/1‑149 (MQ=255)                                                                           gCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTc                                                                      >  1:730418/1‑149 (MQ=255)                                                                                 aTCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCAtt                                                               >  2:925343/1‑150 (MQ=255)                                                                                  tCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTAc                                                             >  2:444929/1‑151 (MQ=255)                                                                                      gCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGtt                                                         >  2:36231/1‑151 (MQ=255)                                                                                      gCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGtt                                                         >  1:479926/1‑151 (MQ=255)                                                                                       ccTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTg                                                        >  1:676215/1‑151 (MQ=255)                                                                                        cTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTg                                                        <  2:783908/150‑1 (MQ=255)                                                                                          gAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGa                                                       <  1:837782/149‑1 (MQ=255)                                                                                          gAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATc                                                     >  1:859579/1‑151 (MQ=255)                                                                                           aaaGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCa                                                    <  2:779936/151‑1 (MQ=255)                                                                                             agagAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCaaa                                                  <  2:596241/151‑1 (MQ=255)                                                                                                 aaTCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCg                                              <  1:1069544/151‑1 (MQ=255)                                                                                                  aTCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCgg                                             >  2:686150/1‑151 (MQ=255)                                                                                                  aTCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCgg                                             >  1:918972/1‑151 (MQ=255)                                                                                                      gCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGTTTGCTCGTCAGGCATTACGGTTGATCAAATCCGGcc                                           <  1:1089891/149‑2 (MQ=255)                                                                                                       ccGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGc                                            <  1:491925/147‑1 (MQ=255)                                                                                                        cGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCt                                        >  2:653391/1‑150 (MQ=255)                                                                                                        cGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTa                                       <  2:569051/151‑1 (MQ=255)                                                                                                          cTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACt                                     >  1:1096559/1‑151 (MQ=255)                                                                                                             aTTACGCTAATCGTGATGATGGCAATGAGCTGGGGTCTTAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTc                                   <  1:847007/150‑1 (MQ=255)                                                                                                               tACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGcc                                >  2:295310/1‑151 (MQ=255)                                                                                                                 cGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCa                               <  1:612629/150‑1 (MQ=255)                                                                                                                 cGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCAt                              >  1:753634/1‑151 (MQ=255)                                                                                                                  gCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCAtt                             <  2:633130/151‑1 (MQ=255)                                                                                                                    tAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTg                            >  2:670857/1‑150 (MQ=255)                                                                                                                      aTCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGaa                          >  1:832564/1‑150 (MQ=255)                                                                                                                              gatgGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATg                 >  2:679429/1‑151 (MQ=255)                                                                                                                               atgGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGa                <  1:107353/151‑1 (MQ=255)                                                                                                                                 ggCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAg               <  1:613107/150‑1 (MQ=255)                                                                                                                                  gCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCg             <  2:150033/151‑1 (MQ=255)                                                                                                                                   cAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGt            <  2:194263/151‑1 (MQ=255)                                                                                                                                    aaTCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTa           <  1:1017183/151‑1 (MQ=255)                                                                                                                                      tCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGc         >  2:33992/1‑151 (MQ=255)                                                                                                                                       cAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGc         <  1:529425/150‑1 (MQ=255)                                                                                                                                        aGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCg       >  2:758809/1‑151 (MQ=255)                                                                                                                                        aGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCg       >  2:1140671/1‑151 (MQ=255)                                                                                                                                         gCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGc      <  1:1012917/151‑1 (MQ=255)                                                                                                                                           tGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCt     >  1:719461/1‑150 (MQ=255)                                                                                                                                             gggTCTGAAGCGCTTCAATCAGCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGCCTGTCCCCGATTGCTCGTCAGGCATTACGGTTGATCAAACCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTAt   <  1:539700/150‑1 (MQ=255)                                                                                                                                             gggTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTAtt  <  1:621718/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                ATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATT  >  NC_000913/3606736‑3607026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑