Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,230	A→G	V135A (GTG→GCG)	mnmG ←	5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,230	0	A	G	100.0%	163.9 / NA	49	V135A (GTG→GCG)	mnmG	5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/21);  total (28/21)

GCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTG  >  NC_000913/3925087‑3925377                                                                                                                                                |                                                                                                                                                    gCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATc                                                                                                                                              >  2:490780/1‑151 (MQ=255) gCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATc                                                                                                                                              <  2:528501/151‑1 (MQ=255)  cGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCg                                                                                                                                             >  2:768937/1‑151 (MQ=255)  cGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCg                                                                                                                                             >  1:322058/1‑151 (MQ=255)     aaTGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGttt                                                                                                                                          <  2:870293/151‑1 (MQ=255)     aaTGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGttt                                                                                                                                          <  1:168398/151‑1 (MQ=255)       tGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTc                                                                                                                                        >  2:645894/1‑151 (MQ=255)         gACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCg                                                                                                                                       >  2:1125482/1‑150 (MQ=255)           cggcggATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGAc                                                                                                                                     >  2:277731/1‑150 (MQ=255)             gcggATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACaa                                                                                                                                   >  1:476965/1‑150 (MQ=255)                     ccAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCtt                                                                                                                          <  1:434516/151‑1 (MQ=255)                           cGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAAcc                                                                                                                     <  2:577830/150‑1 (MQ=255)                           cGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCg                                                                                                                    >  2:949090/1‑151 (MQ=255)                              ccACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCct                                                                                                                 <  2:764274/151‑1 (MQ=255)                                   gCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTgg                                                                                                            >  2:709287/1‑151 (MQ=255)                                      gTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGa                                                                                                           >  1:455866/1‑149 (MQ=255)                                            aTCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACTGCGCGATCGTTTTAGACAATAAGATCTTCAACCGCATGCTGGAAGatcatc                                                                                                   >  1:677513/1‑151 (MQ=255)                                               cAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAgg                                                                                                >  1:1136651/1‑151 (MQ=255)                                               cAGACCGATATGAATTTTACCGTCGAAGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCAATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAgg                                                                                                >  1:295489/1‑151 (MQ=255)                                                   ccGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCg                                                                                            >  1:975034/1‑151 (MQ=255)                                                        atGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCggttgg                                                                                       >  1:547149/1‑151 (MQ=255)                                                           aaTTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTtc                                                                                    <  1:288606/151‑1 (MQ=255)                                                            aTTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTtct                                                                                   >  2:319578/1‑151 (MQ=255)                                                              tttACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTcc                                                                                 >  2:509053/1‑151 (MQ=255)                                                               ttACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTcc                                                                                 <  1:749712/150‑1 (MQ=255)                                                                tACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCa                                                                                <  2:125088/150‑1 (MQ=255)                                                                    gTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCCCCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGcc                                                                           <  2:659359/151‑1 (MQ=255)                                                                          gAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCcgtacgt                                                                     <  2:191392/151‑1 (MQ=255)                                                                           aaCGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCcgtacgt                                                                     >  2:220038/1‑150 (MQ=255)                                                                                cccAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCg                                                                 <  2:257688/149‑1 (MQ=255)                                                                                cccAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGcc                                                               >  2:838226/1‑151 (MQ=255)                                                                                     cGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACg                                                          <  2:1088817/151‑1 (MQ=255)                                                                                     cGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACg                                                          >  2:542311/1‑151 (MQ=255)                                                                                       gTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACgg                                                         >  1:148550/1‑150 (MQ=255)                                                                                           gCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTag                                                      >  2:678037/1‑149 (MQ=255)                                                                                             acgacgGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGc                                                   >  1:627843/1‑150 (MQ=255)                                                                                              cgacgGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGcac                                                 >  2:1111789/1‑151 (MQ=255)                                                                                              cgacgGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGca                                                  <  1:574678/150‑1 (MQ=255)                                                                                                      ttGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATc                                           <  1:490657/149‑1 (MQ=255)                                                                                                        ggCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGcc                                       <  1:162367/151‑1 (MQ=255)                                                                                                        ggCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGc                                        <  2:941927/150‑1 (MQ=255)                                                                                                                 cTTCAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCgg                              >  2:616544/1‑151 (MQ=255)                                                                                                                    cAGTCCCATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGt                            >  1:107346/1‑150 (MQ=255)                                                                                                                        cccATTTGGGTAACAGCCCCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAgcgc                       <  2:477080/151‑1 (MQ=255)                                                                                                                        cccATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAgcgc                       >  1:936478/1‑151 (MQ=255)                                                                                                                         ccATTTGGGTAACAGCACCGACCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGcgcg                      <  2:888104/151‑1 (MQ=255)                                                                                                                              tGGGTAACAGCACCGCCCGCGCGATCGTTTTAGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAAc                   <  1:1037390/149‑1 (MQ=255)                                                                                                                                        ccccGACCGCGCGTTCTTTTTCGAAAATAAGATCTCCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTCCCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCttt       <  2:384087/149‑1 (MQ=255)                                                                                                                                         cccgcccgcGCGATGTTTTCCGAAAAAAAGATTTTCACCCGCCTGCTGGAAGATCATCAGGTTGGGTTGGTTCTCCAGCGCCGTACGTCCCGCCTGACGGTAGAGCACCCGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTttg      <  1:414150/150‑1 (MQ=255)                                                                                                                                             aCCGCGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTttgcttg  >  1:890397/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                    GCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTG  >  NC_000913/3925087‑3925377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑