Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	218,835	(A)8→7	intergenic (‑60/+52)	proS ← / ← tsaA	prolyl‑tRNA synthetase/tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	218,828	0	A	.	100.0%	347.3 / NA	73	intergenic (‑53/+59)	proS/tsaA	prolyl‑tRNA synthetase/tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (35/38);  total (35/38)

CGGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATA  >  NC_000913/218678‑218970                                                                                                                                                       |                                                                                                                                               cGGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACa                                                                                                                                                <  1:494253/151‑1 (MQ=255)   gATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaa                                                                                                                                              <  1:114159/151‑1 (MQ=255)     tcatcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaa                                                                                                                                             >  1:18249/1‑150 (MQ=255)      catcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaa                                                                                                                                           <  2:971623/151‑1 (MQ=255)       atcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaa                                                                                                                                           >  2:529117/1‑150 (MQ=255)       atcCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGGGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaa                                                                                                                                           >  2:485669/1‑150 (MQ=255)         cccGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACaaaaaaag                                                                                                                                         <  2:36600/150‑2 (MQ=255)              cgcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGAttt                                                                                                                                  <  1:950586/151‑1 (MQ=255)               gcgcAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTa                                                                                                                                 <  1:67162/151‑1 (MQ=255)                   agcaTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGttt                                                                                                                             <  1:582380/151‑1 (MQ=255)                      aTCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTAcc                                                                                                                          <  2:600360/151‑1 (MQ=255)                        cAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTAcc                                                                                                                          >  1:718314/1‑149 (MQ=255)                             gATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCa                                                                                                                    <  1:745098/150‑1 (MQ=255)                               tGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCaaa                                                                                                                  >  2:14405/1‑150 (MQ=255)                                 gCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGa                                                                                                               <  1:95182/151‑1 (MQ=255)                                  cTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGa                                                                                                               >  1:21245/1‑150 (MQ=255)                                   tGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGaa                                                                                                              >  2:886796/1‑150 (MQ=255)                                    gATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGaaa                                                                                                             >  2:720585/1‑150 (MQ=255)                                       cACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGt                                                                                                          <  1:450770/150‑1 (MQ=255)                                       cACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGt                                                                                                          <  1:705015/150‑1 (MQ=255)                                        aCCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCa                                                                                                        <  2:412672/151‑1 (MQ=255)                                            cGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCaaa                                                                                                      <  2:114028/149‑1 (MQ=255)                                             ggCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCaaa                                                                                                      >  1:501347/1‑148 (MQ=255)                                               cGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAgaga                                                                                                 <  2:8717/151‑1 (MQ=255)                                                gTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGaa                                                                                                <  2:772695/151‑1 (MQ=255)                                                gTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGaa                                                                                                <  2:625929/151‑1 (MQ=255)                                                   ggCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAggg                                                                                             >  1:179463/1‑151 (MQ=255)                                                    gCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAggg                                                                                             >  2:356833/1‑150 (MQ=255)                                                    gCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGc                                                                                            <  2:386566/151‑1 (MQ=255)                                                     cAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCg                                                                                           >  2:215864/1‑151 (MQ=255)                                                      aGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCg                                                                                           >  2:459437/1‑150 (MQ=255)                                                       ggTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTg                                                                                         <  2:20488/151‑1 (MQ=255)                                                        gtgtCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGa                                                                                        <  2:295952/151‑1 (MQ=255)                                                        gtgtCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGa                                                                                        <  1:670666/151‑1 (MQ=255)                                                              ccTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTa                                                                                   >  2:400415/1‑150 (MQ=255)                                                              ccTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTa                                                                                   <  2:296277/150‑1 (MQ=255)                                                                ttGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAAcg                                                                                >  1:511983/1‑151 (MQ=255)                                                                 tGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAAcg                                                                                <  2:376125/150‑1 (MQ=255)                                                                  gagaGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAAcgc                                                                               <  1:125082/150‑1 (MQ=255)                                                                    gagTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGt                                                                            >  1:995390/1‑151 (MQ=255)                                                                        ggAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTcc                                                                         >  2:411991/1‑150 (MQ=255)                                                                         gagaGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAg                                                                       <  2:610743/151‑1 (MQ=255)                                                                          agagCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAgc                                                                      >  1:226500/1‑151 (MQ=255)                                                                             gCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCa                                                                   <  2:99673/151‑1 (MQ=255)                                                                               aGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCaa                                                                  >  2:321641/1‑150 (MQ=255)                                                                                ggTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCaa                                                                  >  2:615412/1‑149 (MQ=255)                                                                                     tGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTc                                                           >  2:651869/1‑151 (MQ=255)                                                                                     tGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTc                                                           >  2:935885/1‑151 (MQ=255)                                                                                                  tgttgtTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCg                                              >  2:570345/1‑151 (MQ=255)                                                                                                  tgttgtTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCg                                              >  2:639813/1‑151 (MQ=255)                                                                                                   gttgttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCgg                                             <  1:886877/151‑1 (MQ=255)                                                                                                   gttgttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCgg                                             <  2:46049/151‑1 (MQ=255)                                                                                                   gttgttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCg                                              <  2:503267/150‑1 (MQ=255)                                                                                                     tgttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGt                                            <  1:854490/150‑1 (MQ=255)                                                                                                      gttACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTg                                           <  2:901332/150‑1 (MQ=255)                                                                                                        tACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAc                                         <  1:62818/150‑1 (MQ=255)                                                                                                         aCGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGAcgc                                       <  2:976002/151‑1 (MQ=255)                                                                                                           ggTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACgcgc                                     >  2:150605/1‑151 (MQ=255)                                                                                                            gTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGcc                                    >  1:523651/1‑151 (MQ=255)                                                                                                               ccAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGc                                 <  1:317071/151‑1 (MQ=255)                                                                                                                 aGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGa                               <  1:199815/151‑1 (MQ=255)                                                                                                                   ttGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAAc                             >  2:224619/1‑151 (MQ=255)                                                                                                                    tGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACg                            >  2:458016/1‑151 (MQ=255)                                                                                                                        aGGTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTa                         >  1:770596/1‑150 (MQ=255)                                                                                                                         ggaaGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaa                        >  2:57834/4‑150 (MQ=255)                                                                                                                         ggTAGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTaaa                       <  1:10107/151‑1 (MQ=255)                                                                                                                            aGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAAt                     <  1:553900/150‑1 (MQ=255)                                                                                                                            aGAACAGGCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATc                    >  1:859243/1‑151 (MQ=255)                                                                                                                                  ggCTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAg              <  1:356876/151‑1 (MQ=255)                                                                                                                                    cTCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGcc            >  1:18387/1‑151 (MQ=255)                                                                                                                                     tCAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGcc            >  2:125591/1‑150 (MQ=255)                                                                                                                                      cAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa           <  2:558874/150‑1 (MQ=255)                                                                                                                                      cAAGGCGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAg          <  2:751072/151‑1 (MQ=255)                                                                                                                                           cGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACgg      >  2:270237/1‑150 (MQ=255)                                                                                                                                           cGAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGc     >  2:939994/1‑151 (MQ=255)                                                                                                                                            gAGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGc     >  1:110652/1‑150 (MQ=255)                                                                                                                                             aGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCAt   <  1:481521/151‑1 (MQ=255)                                                                                                                                             aGCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCAt   >  1:997779/1‑151 (MQ=255)                                                                                                                                              gCCTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATa  <  1:14407/151‑1 (MQ=255)                                                                                                                                               ccTGGGAC‑AAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCAt   >  2:163562/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                               CGGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGCGTCGGCAGGTGTCTCCTTGAGAGTGGAGAGCAGGTATTGGCTAGTACGCATGTTGTTACGGTTCCAGTTGGAAGGTAGAACAGGCTCAAGGCGAGCCTGGGACAAAAAAAAGTGATTTAGTTTACCAGTGCAAAAGAAATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATA  >  NC_000913/218678‑218970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑