Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 484,337 (C)6→7 coding (67/3150 nt) acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913484,3311.C98.4% 222.5 / ‑4.0 62coding (73/3150 nt)acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (25/36);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTT  >  NC_000913/484184‑484473
                                                                                                                                                    |                                                                                                                                               
tATCGTTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGGGCCGCCGGCAGTTTGGGGATCGCCAAccc                                                                                                                                              >  2:166738/1‑151 (MQ=255)
    gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccc                                                                                                                                         <  1:959332/151‑2 (MQ=255)
    gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGGTTGAGGATCGCCAGccccccc                                                                                                                                         >  2:462594/1‑150 (MQ=255)
     aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccct                                                                                                                                        <  1:61654/151‑3 (MQ=255)
     aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccct                                                                                                                                        <  1:767936/151‑3 (MQ=255)
        ccGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccctgc                                                                                                                                      <  2:569865/150‑5 (MQ=255)
          gTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGccccccctgcc                                                                                                                                     >  2:959764/1‑144 (MQ=255)
               tatTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACatg                                                                                                                                >  1:744634/1‑150 (MQ=255)
                atTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACatgat                                                                                                                              <  1:474164/151‑1 (MQ=255)
                   cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATgatgat                                                                                                                           >  1:525754/1‑151 (MQ=255)
                      ttCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGGAGGAGGCGGAGATCGTTACTGCCGGCGGGGCAATCGTAGGAGAATGCGCCACCGGCAGTTTGGGGATAGCCAGCCCCCCCTGCCAACATGATGGTAAt                                                                                                                        >  2:34807/1‑151 (MQ=255)
                            aaCCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGa                                                                                                                   <  2:937474/150‑1 (MQ=255)
                              ccTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATc                                                                                                                 >  1:74062/1‑150 (MQ=255)
                                 gtgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCAccc                                                                                                             >  1:755191/1‑151 (MQ=255)
                                   gtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGACCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCAc                                                                                                           <  1:435737/151‑1 (MQ=255)
                                     cACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGc                                                                                                         >  2:895354/1‑151 (MQ=255)
                                     cACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGc                                                                                                         <  1:598027/151‑1 (MQ=255)
                                       ccGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaa                                                                                                       <  2:332391/151‑1 (MQ=255)
                                        cGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaa                                                                                                       >  2:192067/1‑150 (MQ=255)
                                         gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaaa                                                                                                     <  1:978385/151‑1 (MQ=255)
                                         gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaaa                                                                                                     <  1:309924/151‑1 (MQ=255)
                                         gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaaa                                                                                                     <  1:815598/151‑1 (MQ=255)
                                           gtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCaaaaa                                                                                                    <  2:121706/150‑1 (MQ=255)
                                           gtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAAt                                                                                                   <  1:260238/151‑1 (MQ=255)
                                           gtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAAt                                                                                                   <  1:941280/151‑1 (MQ=255)
                                               tGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCggg                                                                                               >  1:282996/1‑151 (MQ=255)
                                                gCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGc                                                                                              <  1:527530/151‑1 (MQ=255)
                                                gCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGc                                                                                              <  1:627186/151‑1 (MQ=255)
                                                  aCTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTTAGGAGCGCCCGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATc                                                                                                  >  2:707259/1‑145 (MQ=255)
                                                    tGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCAACGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGGTCACCCACGCAAAAATCGGGcgat                                                                                           >  2:625666/1‑150 (MQ=255)
                                                     gTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatc                                                                                          <  2:990937/150‑1 (MQ=255)
                                                      ttttCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcg                                                                                         >  2:261305/1‑150 (MQ=255)
                                                       tttCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGcgatcga                                                                                        <  2:648424/150‑1 (MQ=255)
                                                              tCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGaa                                                                                 <  2:941047/150‑1 (MQ=255)
                                                                 gcgcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTa                                                                             <  1:69786/151‑1 (MQ=255)
                                                                  cgcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAAtt                                                                              >  1:848547/1‑149 (MQ=255)
                                                                  cgcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAg                                                                            <  2:460417/151‑1 (MQ=255)
                                                                  cgcCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAg                                                                            <  2:215600/151‑1 (MQ=255)
                                                                    ccGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGc                                                                          >  2:548863/1‑151 (MQ=255)
                                                                     cGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGc                                                                          >  1:235820/1‑150 (MQ=255)
                                                                       gggTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATg                                                                       <  2:915887/151‑1 (MQ=255)
                                                                            ggaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTa                                                                  <  1:453975/151‑1 (MQ=255)
                                                                             gaggCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTaa                                                                 <  2:755107/151‑1 (MQ=255)
                                                                                gCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACgg                                                              <  1:600083/151‑1 (MQ=255)
                                                                                     gaTCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCt                                                         <  2:958421/151‑1 (MQ=255)
                                                                                            aCTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTttaa                                                   <  2:4416/150‑1 (MQ=255)
                                                                                                ccGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTttaagttaa                                              <  1:462645/151‑1 (MQ=255)
                                                                                                ccGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTttaagttaa                                              <  1:983713/151‑1 (MQ=255)
                                                                                                ccGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTttaagtta                                               >  1:43041/1‑150 (MQ=255)
                                                                                                    cggTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACt                                          >  2:611178/1‑151 (MQ=255)
                                                                                                     ggTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACtt                                         <  1:958857/151‑1 (MQ=255)
                                                                                                       tGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAACGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAAACTTgg                                       >  2:568569/1‑151 (MQ=255)
                                                                                                            tCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTg                                   >  1:795598/1‑150 (MQ=255)
                                                                                                             cGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGt                                  <  2:845450/150‑1 (MQ=255)
                                                                                                                aGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCa                               <  2:893214/150‑1 (MQ=255)
                                                                                                                   atatTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCACCCTGCTAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCt                           <  1:166760/151‑1 (MQ=255)
                                                                                                                    tatTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTg                          >  1:319747/1‑151 (MQ=255)
                                                                                                                     atTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGa                         <  2:556088/151‑1 (MQ=255)
                                                                                                                       tGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAg                        <  2:304703/150‑1 (MQ=255)
                                                                                                                              ccGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCtt                >  1:240899/1‑151 (MQ=255)
                                                                                                                              ccGGCAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCtt                <  1:520560/151‑1 (MQ=255)
                                                                                                                                  cAGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCgg            >  2:224239/1‑151 (MQ=255)
                                                                                                                                   aGTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGc           >  1:186790/1‑151 (MQ=255)
                                                                                                                                    gTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGc           >  1:135953/1‑150 (MQ=255)
                                                                                                                                    gTTTGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCt          >  2:800991/1‑151 (MQ=255)
                                                                                                                                       tGAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTg        <  1:263529/150‑1 (MQ=255)
                                                                                                                                        gAGGATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGc       <  1:668263/150‑1 (MQ=255)
                                                                                                                                           gATCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGt   >  1:744031/1‑151 (MQ=255)
                                                                                                                                            aTCGCCAGCCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGtt  <  1:979692/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGCGCCACCGGCAGTTTGAGGATCGCCAGCCCCCCTGCCAACATGATGATAATGGCGATCACCCACGCAAAAATCGGGCGATCGATAAAGAAATTAGGCATGTCTTAACGGCTCCTGTTTAAGTTAAGACTTGGACTGTTCAGGCTGAGCACCGCTTGCGGCTTGCTGGTT  >  NC_000913/484184‑484473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: