| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,351,196 | (G)7→8 | intergenic (‑157/+211) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,351,189 | 1 | . | G | 94.6% | 126.9 / ‑0.8 | 37 | intergenic (‑150/+218) | fabI/ycjD | enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (17/18); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAC > NC_000913/1351040‑1351329 | aGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑a < 1:559237/150‑1 (MQ=255)aGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑a < 2:66541/150‑1 (MQ=255) gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gg < 2:152669/151‑1 (MQ=255) gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gg > 1:695027/1‑151 (MQ=255) ttAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gggg < 1:558042/150‑1 (MQ=255) aaTCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑ggggggg > 2:192920/1‑151 (MQ=255) aaTCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gggggg < 2:722088/150‑1 (MQ=255) aTCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gggggggg > 2:653765/1‑150 (MQ=255) aTCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gggggggg < 2:538151/151‑2 (MQ=255) aTCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gggggggg > 1:253491/1‑150 (MQ=255) ccTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑gggggggg > 1:294246/1‑148 (MQ=255) aTGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCt > 2:16217/1‑150 (MQ=255) aattCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCctct < 2:708017/144‑1 (MQ=255) ggCGATTCGTCTTTTTAGTAAACAGTTCTAACAGCTATACGGTTTTTATAATCAACCTGGCTTTGAGTAGCTATAGTTGCCAGGTCCGTCCGGGGAAGGCTTCGGCGG‑‑GGGGGGGCGCTTTTCCCCTCAGCCTAACCCTCTCCCCAGAggg < 2:180923/151‑1 (MQ=255) cGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc < 1:649290/151‑1 (MQ=255) tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGGGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg < 1:862432/150‑1 (MQ=255) ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg > 2:278045/1‑151 (MQ=255) tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCCCCCTAACCCCCTCCCCACAGGGGCGAGGGGAcc > 1:978514/1‑149 (MQ=255) tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg < 1:218151/150‑1 (MQ=255) tAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa < 1:380377/150‑1 (MQ=255) aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt < 2:332524/151‑1 (MQ=255) aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATtgtg > 2:984326/1‑151 (MQ=255) aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTTCCCTCGACCTAAACCTCTCCCCAGAGGGGCGAgggg > 1:352045/1‑138 (MQ=255) cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCCAACCCCCTCCCCAGAGGGGCGAGGGGACCGTATTGTGc > 1:296753/1‑149 (MQ=255) gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTTCCCTCACCCCAAACCTCCCCCCAGAGGGGCGAGGGGAGCGTATTGTGCaaa > 1:745904/1‑150 (MQ=255) tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAat > 2:335888/1‑150 (MQ=255) aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAata < 2:772283/150‑1 (MQ=255) cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑a < 1:81508/151‑1 (MQ=255) cGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGca < 2:819439/151‑1 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGcaacaa > 2:456715/1‑150 (MQ=255) attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCaacaaa < 2:453173/151‑1 (MQ=255) ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCaacaaaca < 2:393312/150‑1 (MQ=255) ataATCAACCTCTCTGTGAGTAGCTATTGTTGCCAGGTCCGACCGGAGCAGGCTGCGTC‑AG‑GGGGGGGCACTTTTACCCTCACCATAAACATCTACCCAGAGGGGAGAGGGGACAGTATTGTGCAAATATTATT‑ACCCCAGCaaaaaaca < 2:783965/150‑1 (MQ=255) tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGGGGGGACCGTATTGTGCAAATATTGTT‑ACCCCCGCAACAAACAGGCTCATACa > 1:660686/1‑150 (MQ=255) tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTTCCCTCACCCTTACACCCTCCCCCGAGGGGCGGGGGGGCCGGATTTTGCGAATATTGTT‑ATACCCGCAAAAAACAAGCGCATATACcccc > 1:511875/1‑151 (MQ=255) tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCCCCCTAACCCCCCCCCCAGAGGGGCGAGGGGGCCGTATTGGGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAAcccc > 2:816856/1‑151 (MQ=255) aGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCCCCCTAACCCTCTCCCCAGAGGGGCGGGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAACCCCCAAccc > 2:675455/1‑150 (MQ=39) gtattaGTTGCCAGGTCCGCCCGGGGCAGGCGGCGGC‑A‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCttt < 1:192943/147‑1 (MQ=255) cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGGGCCAATATTGTT‑ACCCCAGCAAAAAACAGGCCCATACAGCCCCTAACCCttt > 2:799186/1‑151 (MQ=38) ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTTCCCTCACCCCAACCCCCTCCCCCGAGGGGCGAGGGGAACGCGTTTTGGCAAAATTGTTAACCCCAG‑AACAAACAAGCTCTTAAAGCCCCTAAccc > 1:308004/1‑146 (MQ=255) aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATg < 2:579237/151‑1 (MQ=255) aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCCCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGGCCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAAGCTCATACAACCCCTAACCCATTCATg > 2:573411/1‑151 (MQ=255) gCCAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg < 2:3522/150‑1 (MQ=255) ccAGGTCCGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGAt < 2:172211/151‑1 (MQ=255) cGACCGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTggg < 1:807777/151‑1 (MQ=255) ccGGAGCAGGCTGCGGC‑AGGGGGGGGGCGCTTTTCCCCCCACCCCAAACCCCTCCCCCGAGGGGCGAGGGGGCCCTATTTTGCAAAAATTTTT‑ACCCCAGCAAAAAAAAAGCTCATAAAACCCCCAACCCCTTCATGGGGATGGCTggg > 1:927518/1‑149 (MQ=255) cGGAGCAGGCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGAc < 1:482989/149‑1 (MQ=255) ggCTGCGGC‑AG‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc < 1:212922/151‑1 (MQ=255) | AGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑A‑‑GGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTT‑ACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAC > NC_000913/1351040‑1351329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |