Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,054	(C)7→8	coding (1279/2526 nt)	ecpC ←	ECP production outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,047	1	.	C	100.0%	263.7 / NA	71	A429G (GCG→GGG)	ecpC	ECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (30/41);  total (30/41)

GCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTT  >  NC_000913/307897‑308192                                                                                                                                                        |                                                                                                                                                   gCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCg                                                                                                                                                     <  1:639935/151‑1 (MQ=255)         aCGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑ccccccc                                                                                                                                            <  2:352164/150‑1 (MQ=255)          cGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑cccccccc                                                                                                                                           >  2:588473/1‑149 (MQ=255)           gTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑cccccccca                                                                                                                                          >  1:692972/1‑148 (MQ=255)           gTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑cccccccca                                                                                                                                          >  1:719811/1‑148 (MQ=255)             cGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGTGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑ccccccccaag                                                                                                                                        <  1:677889/150‑5 (MQ=255)             cGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑ccccccccaag                                                                                                                                        <  1:806146/150‑5 (MQ=255)                 attgattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCa                                                                                                                                    <  1:283582/151‑1 (MQ=255)                   tgattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAg                                                                                                                                   <  1:181301/150‑1 (MQ=255)                    gattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGc                                                                                                                                  <  1:90706/150‑1 (MQ=255)                    gattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCg                                                                                                                                 <  2:499012/151‑1 (MQ=255)                    gattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCg                                                                                                                                 <  1:623076/151‑1 (MQ=255)                    gattgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCg                                                                                                                                 >  2:703688/1‑151 (MQ=255)                     attgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGt                                                                                                                                >  1:153522/1‑151 (MQ=255)                     attgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGt                                                                                                                                >  1:40562/1‑151 (MQ=255)                     attgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGt                                                                                                                                >  2:688032/1‑151 (MQ=255)                     attgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGt                                                                                                                                <  1:548127/151‑1 (MQ=255)                       tgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGCCCCCCCCCAAGCGGCAGCGt                                                                                                                                <  1:69864/150‑1 (MQ=255)                       tgCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTc                                                                                                                               <  1:305498/150‑1 (MQ=255)                        gCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTc                                                                                                                               >  1:27134/1‑149 (MQ=255)                         ccAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGa                                                                                                                            <  2:367749/151‑1 (MQ=255)                               cGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGtt                                                                                                                      <  1:480478/151‑1 (MQ=255)                                 ggTTTTTTCCTGATTAGCCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTc                                                                                                                    <  2:165105/151‑1 (MQ=255)                                       ttCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTcacccac                                                                                                              >  2:628755/1‑151 (MQ=255)                                       ttCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTcaccca                                                                                                               <  2:312114/150‑1 (MQ=255)                                         ccTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCg                                                                                                            <  1:245321/151‑1 (MQ=255)                                            gATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGcc                                                                                                          <  1:425730/150‑1 (MQ=255)                                            gATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGc                                                                                                           <  1:524832/149‑1 (MQ=255)                                                aaCCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGAtt                                                                                                     <  2:719734/151‑1 (MQ=255)                                                 aCCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGAtt                                                                                                     >  2:433930/1‑150 (MQ=255)                                                 aCCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGAtt                                                                                                     >  1:117734/1‑150 (MQ=255)                                                  cccACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTAt                                                                                                   <  1:376616/151‑1 (MQ=255)                                                       aGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTAt                                                                                              <  1:998228/151‑1 (MQ=255)                                                         cGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATcc                                                                                            <  2:850642/151‑1 (MQ=255)                                                          gAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATcc                                                                                            >  1:369801/1‑150 (MQ=255)                                                          gAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATcc                                                                                            >  2:559695/1‑150 (MQ=255)                                                          gAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATc                                                                                             >  1:317140/1‑149 (MQ=255)                                                          gAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCa                                                                                           <  1:391796/151‑1 (MQ=255)                                                          gAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCa                                                                                           <  1:692244/151‑1 (MQ=255)                                                           aaCTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCat                                                                                          >  1:640967/1‑151 (MQ=255)                                                           aaCTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCat                                                                                          <  2:707527/151‑1 (MQ=255)                                                              tAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATc                                                                                       >  2:414785/1‑151 (MQ=255)                                                               aaaGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCatat                                                                                        <  1:658975/149‑1 (MQ=255)                                                                  gCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGt                                                                                   >  1:739570/1‑151 (MQ=255)                                                                       ccGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccg                                                                              <  1:192766/151‑1 (MQ=255)                                                                         ggTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTgccgcc                                                                            >  2:186144/1‑151 (MQ=255)                                                                            agagTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCa                                                                          <  1:213426/150‑1 (MQ=255)                                                                            agagTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAg                                                                         <  2:997116/151‑1 (MQ=255)                                                                            agagTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAg                                                                         <  2:680793/151‑1 (MQ=255)                                                                                           ctgcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGCTTTTCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAAt                                                          >  1:890957/1‑151 (MQ=255)                                                                                             gcCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGa                                                        <  1:525054/151‑1 (MQ=255)                                                                                              ccGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATg                                                         >  2:247274/1‑149 (MQ=255)                                                                                              ccGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAg                                                       <  1:113529/151‑1 (MQ=255)                                                                                                gATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGcc                                                     >  2:522346/1‑151 (MQ=255)                                                                                                    ctgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCgg                                                   >  1:51228/1‑149 (MQ=255)                                                                                                    ctgctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCgg                                                   >  2:295535/1‑149 (MQ=255)                                                                                                      gctCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGt                                               <  1:756438/151‑1 (MQ=255)                                                                                                          cATGAGCTGTCCCTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTc                                              <  2:225555/148‑1 (MQ=255)                                                                                                          cATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCg                                             >  1:813884/1‑149 (MQ=255)                                                                                                                cTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCt                                     >  1:110827/1‑151 (MQ=255)                                                                                                                  gTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTg                                    <  2:247908/150‑1 (MQ=255)                                                                                                                    cACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTa                                 <  2:117689/151‑1 (MQ=255)                                                                                                                    cACTGGCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTa                                 <  2:757604/151‑1 (MQ=255)                                                                                                                        ggCCAGCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGc                               <  1:994901/149‑1 (MQ=255)                                                                                                                             gCATATTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAActct                        >  1:603850/1‑151 (MQ=255)                                                                                                                               atatTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAActctct                      >  1:338955/1‑151 (MQ=255)                                                                                                                                 atTTTGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCtt                     <  1:844481/150‑1 (MQ=255)                                                                                                                                   tttGCAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCttt                    >  2:373893/1‑149 (MQ=255)                                                                                                                                       cAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTgg              <  2:296521/151‑1 (MQ=255)                                                                                                                                       cAGGTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTgg              >  2:89625/1‑151 (MQ=255)                                                                                                                                         ggTTAACGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTc            <  1:478490/151‑1 (MQ=255)                                                                                                                                              aCGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTc       >  1:761651/1‑151 (MQ=255)                                                                                                                                               cGTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCt      <  2:920291/151‑1 (MQ=255)                                                                                                                                                gTTGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCtt     <  2:266303/151‑1 (MQ=255)                                                                                                                                                 ttGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCttt    >  2:176525/1‑151 (MQ=255)                                                                                                                                                  tGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCtttt   >  2:168297/1‑151 (MQ=255)                                                                                                                                                  tGATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATAGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCttt    <  1:588536/150‑1 (MQ=255)                                                                                                                                                   gATCGC‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCttttt  >  1:809340/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                   GCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGCCAGCATATTTTGCAGGTTAACGTTGATCG‑‑CCCCCCCAAGCGGCAGCGTCAGACGGGTTTCACCCACCGCCTGATTATCGTATCCATATCCCGTTGCCGCCCAGCTAAGCGTACTCAATGAGCCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTT  >  NC_000913/307897‑308192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑