Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 213,341 | 0 | A | G | 44.6% | 80.1 / 14.2 | 57 | G337G (GGA→GGG) | tilS | tRNA(Ile)‑lysidine synthetase |
Reads supporting (aligned to +/- strand): ref base A (5/26); new base G (25/0); total (31/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.73e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC > NC_000913/213195‑213490 | gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgt < 1:157027/150‑1 (MQ=255) gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgt < 2:846543/150‑1 (MQ=255) ccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGc > 2:632470/1‑150 (MQ=255) ccTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCt < 1:749766/151‑1 (MQ=255) gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCtt < 2:634374/149‑1 (MQ=255) tttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTaa > 1:513402/1‑150 (MQ=255) aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGTAGGGTACAGATTaaagtg > 2:535641/1‑147 (MQ=255) gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGCg > 1:738962/1‑148 (MQ=255) gAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGc < 2:413304/150‑1 (MQ=255) aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAAGCGGGAGGCGGTATTTGccc > 1:897263/1‑150 (MQ=255) ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCcg < 1:280631/151‑1 (MQ=255) cTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGCGGGAGGGGATATTCGCCCTCcccgcg > 1:651558/1‑147 (MQ=255) aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCAGGGGGGAGGACCCCTTAATGCGGGGGGGGGCATTTTCCCTCcccggg > 2:908130/1‑145 (MQ=255) cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACg < 2:511294/151‑1 (MQ=255) gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAATGCGGGGGGGGGTATTTGCCCTCCCCGCGCAGACGAAGc > 1:864467/1‑150 (MQ=255) gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCg < 2:72974/151‑1 (MQ=255) aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTc < 2:847463/151‑1 (MQ=255) aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTc < 2:453971/151‑1 (MQ=255) cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAAGCGGGGGGCGGGATTCGCCCCCCCCCGGGAGACGAAGCgg > 2:55271/1‑148 (MQ=255) gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATTCGGGGGGGGATATTTGCCCTCCCCGGGCAGACGAAACg > 2:398102/1‑145 (MQ=255) tggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGGGGATAGCTTAATACGGGGGGGGGTATTTGCCCCCCGCGTGCAGAAGAAGAgggtag > 2:555591/1‑145 (MQ=255) ggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc‑g < 1:3609/150‑1 (MQ=255) gtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGGGGCGATATTTTCCCTCCGCGTGCAGACGAAACGGTCAg > 1:129565/1‑147 (MQ=255) gATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc‑gtgc‑gt < 2:867793/151‑1 (MQ=255) aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTc < 1:90823/150‑1 (MQ=255) aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCGTAATACGGGAGGGGATATTCGCCCTCCGCGGGCAGACGAAGCGGGCAGC‑GGGC‑GGTTTaa > 1:896864/1‑150 (MQ=255) aTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCaaa < 2:816864/151‑1 (MQ=255) ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGGTTCATCCCGGAGGGGACATTTGCCCCCCGCCGGCAGGAGAAACggggcacgggcggtt > 1:583529/1‑132 (MQ=255) cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGcc < 1:826569/150‑1 (MQ=255) cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAATGCGGGAGGGGATATTTGCCCTCCGCGGGCAGACGAAGCGGTCAGC‑GGGC‑GGTTCAAAACGCCAgg > 1:497181/1‑150 (MQ=255) cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCat < 2:585252/151‑1 (MQ=255) gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTTAACCGGGGGGGGATATTCTGCCCCCGCGTGCAGGCGAAGGGGTCAGC‑GGGC‑GGTTCAAAGAGCCCGGGTTGCCGCat > 1:520153/1‑150 (MQ=255) aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAAATCGGGGGGGGGTATTCGCCCCCCCCGTGCAGACGAAGCGGGCAGCGGGGG‑GTTTAAAAGGGCC‑GGGGTGCTGCCTAtttt > 1:798718/1‑149 (MQ=255) aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAATGCGGGGGGCGGTATTTGCCCTCCCCGGGCAGACGGAGCGGTCAGC‑GGGC‑GTTTCAAAACGCCCGGGTTGCTGCCTATTGt > 2:378796/1‑151 (MQ=255) aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGCGGGGGGGGATATTTGCCCTCCCCGTGCAGACGAAGCGGGCAGC‑GTGC‑GGTTTAAAACGCCAGGGTTGCTGCATAtttt > 1:545040/1‑147 (MQ=255) aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCg < 1:632535/151‑1 (MQ=255) ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTTCAGCTTAAAGCGGGAGGCGGTATTCGCCCTCCGCGGGCCGACGAAGCGGGCAGC‑GGGC‑GGTTTAAAGAGCCAGGAGTGTTGCCGATTGt > 1:633005/1‑148 (MQ=255) gCCGTGGCAGACGTGGCTTCACCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGt < 1:463072/151‑1 (MQ=255) gCCGTGGCAGACGTGGCGTCAGCCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAGGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCTGTCGGC‑GTGC‑GTTACAAAGCGCCAGGATTGCTGCATATTGTCGGGCGt < 2:751059/151‑1 (MQ=255) ccGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGAGGGGATATTCGCCCCCCGCGTGCAGACGGAGGGGGTAGC‑GTGC‑GGTTCAAAGCGCCAGGAATGCTGCCTATTGTCGGGCg > 1:112429/1‑149 (MQ=255) tGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACCGCTTAATGCGGGGGGGGATATTCGCCCTCCGCGTGCAGACGAAGCGGGCAGC‑GTGCGGTTTCAAA‑CGCCAGGGTTGCTGCCTATTGTTggggggaa > 1:840706/1‑144 (MQ=255) ggCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGAAAGCTTAAAGCGGGGGGGGAAATTCGGCCCCCGCGGGCAGAAGAAGCGGGCAGG‑GGGC‑GGTTTAAAAAGGCAGGAATGCTGCATATTGTCGGGGGTAAcgg > 1:225022/1‑151 (MQ=255) aCGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATTCGGGGGGGGATATTTGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GGGC‑GGTTCAAAACGCCAGGATTGCTGCATATTTTCGGGCGGAAaggcgg > 1:201846/1‑149 (MQ=255) gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGt < 2:513346/151‑1 (MQ=255) gTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCCCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGt < 2:18493/151‑1 (MQ=255) ggCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTa < 1:638873/150‑1 (MQ=255) ttCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGCGGGAGGGGGTATTCGCCCTCCCCGTGCAGACGAAACGGTCAGC‑GGGC‑GGTTCAAAACGCCCGGATTGCTGCATATTTTCGGGCGGAACGGCGGGCGGAAGCt > 1:23515/1‑151 (MQ=255) ttCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAATCGGGGGGCGGTATTCCCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAACGCCAGGGTTGCTGCATATTGTCGGGGGGAACGGGGGGCGtgaaat > 2:493942/1‑146 (MQ=255) tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGc < 2:696575/149‑1 (MQ=255) ccGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGa < 1:192360/151‑1 (MQ=255) ccGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCa > 1:453783/1‑149 (MQ=255) cggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAGCGGCGGACGTAAGCTAAAGAAAATCTGGCAaga < 1:128268/151‑1 (MQ=255) ggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAagag < 2:798635/151‑1 (MQ=255) ggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTggg > 1:711173/1‑151 (MQ=255) tGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCg > 1:392689/1‑150 (MQ=255) gAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgcc > 2:372971/1‑151 (MQ=255) gAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgc > 1:627290/1‑150 (MQ=255) | GCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCC > NC_000913/213195‑213490 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |