Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,6920CA43.1% 79.6 / 42.2 58intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (5/28);  new base A (25/0);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 8.35e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAC  >  NC_000913/2903542‑2903840
                                                                                                                                                       |                                                                                                                                                       
gTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAc                                                                                                                                                         <  2:153192/151‑1 (MQ=255)
    ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACa                                                                                                                                                       >  1:958518/1‑149 (MQ=255)
       cAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAacacac                                                                                                                                                    >  1:14815/1‑147 (MQ=255)
         aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTa                                                                                                                                               <  1:400040/151‑1 (MQ=255)
          ggAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACACTCAtt                                                                                                                                                >  2:162746/1‑149 (MQ=255)
             atatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCt                                                                                                                                          <  1:460556/151‑1 (MQ=255)
                  aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaa                                                                                                                                      <  1:378445/150‑1 (MQ=255)
                   aCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACTCATTAACCCCaaaa                                                                                                                                     >  1:81456/1‑150 (MQ=255)
                                    tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTAATGTTAAACCCACCCTTCGCCCCGAAACAAAAAAAAAATTcctg                                                                                                                    >  1:840795/1‑148 (MQ=255)
                                          aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTTTTTTAAAAAAACCATTTTCCTCCAAAAAAAAAAAAAAATCCAGATttatt                                                                                                            >  2:176044/1‑151 (MQ=255)
                                          aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAACCCATTAACCTCAAAAAAAAAACAAAATTCCAGAtttttt                                                                                                            >  1:460797/1‑151 (MQ=255)
                                              gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACtt                                                                                                        <  2:687945/151‑1 (MQ=255)
                                                  aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACAAACATTATCCCCAAAACAAAAAAAAAAATCCACATTTATTAAtt                                                                                                        >  2:779049/1‑147 (MQ=255)
                                                  aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACAACCATTAAGCTCAAAAAAAAAAAAAAATTTCAAAGttatt                                                                                                            >  1:104567/1‑143 (MQ=255)
                                                    aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCa                                                                                                   <  1:176066/150‑1 (MQ=255)
                                                       aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATATAAAAACACCCATTATCCTCCAAAAAAAAAAAAAATTTCAGATTTATTACTTAATCaa                                                                                                  >  1:422987/1‑148 (MQ=255)
                                                          cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCATTTACCTCAAAAAAAAAAAAAAATTCCCGATTTTTTACTTAAACaaaaaa                                                                                              >  1:277951/1‑145 (MQ=255)
                                                                ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACAATCCTTAACCCCCAAAAAAAAAAAAAAATTCAGATTTATTAATTTAACAAAGAATAaaaa                                                                                        >  1:254586/1‑149 (MQ=255)
                                                                  aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCtt                                                                                    <  2:498691/151‑1 (MQ=255)
                                                                   tCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTTAACCCCAAAAAAAAAAAAAATTCCCAATTTTTTTATTAAACCattaataaaaaacat                                                                                     >  1:593098/1‑136 (MQ=255)
                                                                   tCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCATTTACCTCAAAAAAAAAAAAAAAATCCAGATTTTTTTATTAAACAATAAATAaaaaaatt                                                                                    >  1:958592/1‑146 (MQ=255)
                                                                     aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaa                                                                                 <  2:73040/151‑1 (MQ=255)
                                                                     aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaa                                                                                 <  2:16474/151‑1 (MQ=255)
                                                                        tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaaaa                                                                               <  1:605958/150‑1 (MQ=255)
                                                                           cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACACAACTTTTCCTCCAAAAAAAAAAAAAACTCCCGAATTTTTTCTTTAACAATGAAAAAAAAAGATaaaaaa                                                                              >  2:979824/1‑148 (MQ=255)
                                                                               tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATAc                                                                         <  1:631729/149‑1 (MQ=255)
                                                                                 aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGtt                                                                     <  2:603584/151‑1 (MQ=255)
                                                                                  aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGt                                                                      <  2:461821/149‑1 (MQ=255)
                                                                                  aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTAACCTCAAAACAAAAACAAAATTCCAGATTTTTTATTTAAACAAAGAAAAAAAAACTTTAAAAAAATACTGt                                                                      >  1:461872/1‑149 (MQ=255)
                                                                                   gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACACACATTAACCTCCAAACAAAAAAAAACTTCCAGATTTTTTTCTTAAACAAAGAATAACAAAACTAAAAAAAATAATGtttt                                                                   >  2:933402/1‑149 (MQ=255)
                                                                                   gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACAAACATTAACCCCCAAAAAAAAACAAAAATCCAGATTTTTTTTTTAAACAAAGAAAAAAAAACTTTAAAAAAAAAATGtt                                                                     >  1:813730/1‑149 (MQ=255)
                                                                                    tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACCCATTAACCCCCAAAAAAAAACAAAATTCCCAATTTTTTTTTTTAACAAATAATAAAAAACTTAAAAAAaataattttttt                                                                  >  2:848842/1‑143 (MQ=255)
                                                                                      tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGGTGTGTTAAAACCCACAATTTACTCAAAAAAAAAAAAAACTTTCCCAGTTTTTTAATAAACAAAGAAAAAAAAACTTTAAAAAAATACTAttt                                                                    >  1:117986/1‑146 (MQ=255)
                                                                                      tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTTTATAAAAAAACAAAATTTCGTTAAAAAAAAAAAAAAATTCCCCATTTATTATTTTAACAAAGAAAAAAAAAATAAAAAAAAAAAATattatttt                                                                >  2:594714/1‑149 (MQ=255)
                                                                                       gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCt                                                                <  2:87250/150‑1 (MQ=255)
                                                                                         aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaaa                                                             <  1:34678/151‑1 (MQ=255)
                                                                                           tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAAAAAACAATAACCTCCAAAAAAAAACAAACCTTCAAATTTTTTAAATTAACAAAAAAAAAAAAAATTAAAAAAAAAAATTTTATTTTaaaa                                                            >  2:152075/1‑149 (MQ=255)
                                                                                                     tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACACATTATCCCCCAAAAAAAAAAAAAAATTCCGAATTTTTTCTTTAACAAAAAAAAAAAAAATTAAAAAAAAAAATTTTTTTTTTAAAAAAAAAAta                                                    >  1:572124/1‑148 (MQ=255)
                                                                                                      aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACa                                                <  1:179788/151‑1 (MQ=255)
                                                                                                          aTAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCa                                            <  2:81445/151‑1 (MQ=255)
                                                                                                          aTAAGAACTATTCATCAACAGCGTATAGAGGCGGATCAGTCAAAACACTGACTAATCTCCAAAACAAGAAAAACGTCCAGAATTTTTTAATTAACAACGAAAAACAAATCAAAAAAAAATACAGTTTTTTTAAATAAAAAAATaaaaaac                                             >  1:81011/1‑145 (MQ=255)
                                                                                                            aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt                                          <  2:742826/151‑1 (MQ=255)
                                                                                                            aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAt                                           <  2:239434/150‑1 (MQ=255)
                                                                                                                   aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                     <  1:894898/149‑1 (MQ=255)
                                                                                                                     tcatcaACAGCGTATAGAGGCGGTTATTTAAAAACACACATTAACCTCAAAAAAAAAACAAAAATCCAGATTTTTTTCTTAAACAAAGAATAAAAAACTTTAAAAAAAAAATTTTTTTTTTAAAAAAAAAATAAAAAGCAATaa                                        >  1:206105/1‑144 (MQ=255)
                                                                                                                      catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat                                <  2:70785/151‑1 (MQ=255)
                                                                                                                      catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat                                <  1:611417/151‑1 (MQ=255)
                                                                                                                      catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAata                                 <  2:788080/150‑1 (MQ=255)
                                                                                                                        tcaACAGCGTATAGAGGCGGTTTTGTAAAAACACTCATTATTCCCCAAAAAAAAAAAAACATCCAGATTTATTTTTTAAACAAAAAATAAAAAACTTAAAAAAAATAATTTTTTTTTTAAAAAAAAAATAAAAAACATTAATTaaa                                   >  2:195105/1‑146 (MQ=255)
                                                                                                                           aCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCGATATTaaaa                           <  1:673452/151‑1 (MQ=255)
                                                                                                                                  ataGAGGCGGTTATGTAAAAACAACCACTTGCCTCCAAAAAAAAAAAAAAATCCCCATTTTTTTTTTTAACAAAAAAAAAAAAACTTAAAAAAAATAATGTTATTTTTTAAAAAAAAATAAAAAGCATTAATTAAATATTaaaaaaaaaa                     >  2:332257/1‑150 (MQ=255)
                                                                                                                                    agagGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAataata                   <  2:141078/150‑1 (MQ=255)
                                                                                                                                     gagGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGa                 <  2:161814/151‑1 (MQ=255)
                                                                                                                                         cGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaa             <  1:787591/151‑1 (MQ=255)
                                                                                                                                             ttATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaa                          <  1:944930/135‑1 (MQ=255)
                                                                                                                                             ttATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaa                          >  2:944846/1‑135 (MQ=255)
                                                                                                                                               aTGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt        <  2:890323/151‑1 (MQ=255)
                                                                                                                                                 gTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCt      >  2:703319/1‑151 (MQ=255)
                                                                                                                                                    aaaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTa    >  2:575520/1‑150 (MQ=255)
                                                                                                                                                     aaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc  >  2:793782/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAC  >  NC_000913/2903542‑2903840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: