Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,903,692 | 0 | C | A | 43.1% | 79.6 / 42.2 | 58 | intergenic (+318/+1055) | ygcE/queE | putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis |
Reads supporting (aligned to +/- strand): ref base C (5/28); new base A (25/0); total (30/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.35e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 |
GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAC > NC_000913/2903542‑2903840 | gTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAAc < 2:153192/151‑1 (MQ=255) ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTAAAAACa > 1:958518/1‑149 (MQ=255) cAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAacacac > 1:14815/1‑147 (MQ=255) aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTa < 1:400040/151‑1 (MQ=255) ggAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAACACTCA‑tt > 2:162746/1‑149 (MQ=255) atatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCt < 1:460556/151‑1 (MQ=255) aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCaaa < 1:378445/150‑1 (MQ=255) aCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAACACTCA‑TTA‑ACCCCaaaa > 1:81456/1‑150 (MQ=255) tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TAATGTTAAACCCACCC‑TTC‑GCCCCGAAACAAAAAAAAAATTcctg > 1:840795/1‑148 (MQ=255) aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTTTTTTAAAAAAAC‑CA‑TTT‑TCCTCCAAAAAAAAAAAAAAATCCAG‑ATttatt > 2:176044/1‑151 (MQ=255) aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAAAAACCCA‑TTA‑ACCTCAAAAAAAAAACAAAATTCCAG‑Atttttt > 1:460797/1‑151 (MQ=255) gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACtt < 2:687945/151‑1 (MQ=255) aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAACAAACA‑TTA‑TCCCCAAAACAAAAAAAAAAATCCAC‑ATTTATTAAtt > 2:779049/1‑147 (MQ=255) aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTTAAAACAACCA‑TTA‑AGCTCAAAAAAAAAAAAAAATTTCAA‑AGttatt > 1:104567/1‑143 (MQ=255) aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCa < 1:176066/150‑1 (MQ=255) aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATATAAAAACACCCA‑TTA‑TCCTCCAAAAAAAAAAAAAATTTCAG‑ATTTATTACTTAATCaa > 1:422987/1‑148 (MQ=255) cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAACACCCA‑TTT‑ACCTCAAAAAAAAAAAAAAATTCCCG‑ATTTTTTACTTAAACaaaaaa > 1:277951/1‑145 (MQ=255) ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTTAAAACAATCC‑TTA‑ACCCCCAAAAAAAAAAAAAAATTCAG‑ATTTATTAATTTAACAAAGAATAaaaa > 1:254586/1‑149 (MQ=255) aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCtt < 2:498691/151‑1 (MQ=255) tCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAACACCCATTTA‑ACCCCAAAAAAAAAAAAAATTCCCAA‑TTTTTTTATTAAACCattaataaaaaacat > 1:593098/1‑136 (MQ=255) tCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAACACCCA‑TTT‑ACCTCAAAAAAAAAAAAAAAATCCAG‑ATTTTTTTATTAAACAATAAATAaaaaaatt > 1:958592/1‑146 (MQ=255) aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTaaa < 2:73040/151‑1 (MQ=255) aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTaaa < 2:16474/151‑1 (MQ=255) tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTaaaaa < 1:605958/150‑1 (MQ=255) cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTAAAAACACAAC‑TTT‑TCCTCCAAAAAAAAAAAAAACTCCCG‑AATTTTTTCTTTAACAATGAAAAAAAAAGATaaaaaa > 2:979824/1‑148 (MQ=255) tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATAc < 1:631729/149‑1 (MQ=255) aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGtt < 2:603584/151‑1 (MQ=255) aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGt < 2:461821/149‑1 (MQ=255) aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAAACACTCA‑TTA‑ACCTCAAAACAAAAACAAAATTCCAG‑ATTTTTTATTTAAACAAAGAAAAAAAAACTTTAAAAAAATACTGt > 1:461872/1‑149 (MQ=255) gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAACACACA‑TTA‑ACCTCCAAACAAAAAAAAACTTCCAG‑ATTTTTTTCTTAAACAAAGAATAACAAAACTAAAAAAAATAATGtttt > 2:933402/1‑149 (MQ=255) gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTTAAAAACAAACA‑TTA‑ACCCCCAAAAAAAAACAAAAATCCAG‑ATTTTTTTTTTAAACAAAGAAAAAAAAACTTTAAAAAAAAAATGtt > 1:813730/1‑149 (MQ=255) tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTTAAAAAACCCA‑TTA‑ACCCCCAAAAAAAAACAAAATTCCCA‑ATTTTTTTTTTTAACAAATAATAAAAAACTTAAAAAAaataattttttt > 2:848842/1‑143 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑GTGTGTTAAAACCCACA‑ATT‑TACTCAAAAAAAAAAAAAACTTTCCC‑AGTTTTTTAATAAACAAAGAAAAAAAAACTTTAAAAAAATACTAttt > 1:117986/1‑146 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑CTTTATAAAAAAACAAA‑ATT‑TCGTTAAAAAAAAAAAAAAATTCCCC‑ATTTATTATTTTAACAAAGAAAAAAAAAATAAAAAAAAAAAATattatttt > 2:594714/1‑149 (MQ=255) gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCt < 2:87250/150‑1 (MQ=255) aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaaa < 1:34678/151‑1 (MQ=255) tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGTTAAAAAAAACA‑ATA‑ACCTCCAAAAAAAAACAAACCTTCAA‑ATTTTTTAAATTAACAAAAAAAAAAAAAATTAAAAAAAAAAATTTTATTTTaaaa > 2:152075/1‑149 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTTAAAAACACACA‑TTATCCCCCAAAAAAAAAAAAAAATTCCGA‑ATTTTTTCTTTAACAAAAAAAAAAAAAATTAAAAAAAAAAATTTTTTTTTTAAAAAAAAAAta > 1:572124/1‑148 (MQ=255) aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACa < 1:179788/151‑1 (MQ=255) aTAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCa < 2:81445/151‑1 (MQ=255) aTAAGAACTATTCATCAACAGCGTATAGAGGCGG‑ATCAGTCAAAACACTGA‑CTA‑ATCTCCAAAACAAGAAAAACGTCCAGAATTTTTTAATTAA‑CAACGAAAAACAAATCAAAAAAAAATACAGTTTTTTTAAATAAAAAAATaaaaaac > 1:81011/1‑145 (MQ=255) aaGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt < 2:742826/151‑1 (MQ=255) aaGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAt < 2:239434/150‑1 (MQ=255) aTTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc < 1:894898/149‑1 (MQ=255) tcatcaACAGCGTATAGAGGCGG‑TTATTTAAAAACACACA‑TTA‑ACCTCAAAAAAAAAACAAAAATCCAG‑ATTTTTTTCTTAAACAAAGAATAAAAAACTTTAAAAAAAAAATTTTTTTTTTAAAAAAAAAATAAAAAGCAATaa > 1:206105/1‑144 (MQ=255) catcaACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat < 2:70785/151‑1 (MQ=255) catcaACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat < 1:611417/151‑1 (MQ=255) catcaACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAata < 2:788080/150‑1 (MQ=255) tcaACAGCGTATAGAGGCGG‑TTTTGTAAAAACACTCA‑TTA‑TTCCCCAAAAAAAAAAAAACATCCAG‑ATTTATTTTTTAAACAAAAAATAAAAAACTTAAAAAAAATAATTTTTTTTTTAAAAAAAAAATAAAAAACATTAATTaaa > 2:195105/1‑146 (MQ=255) aCAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCGATATTaaaa < 1:673452/151‑1 (MQ=255) ataGAGGCGG‑TTATGTAAAAACAACCA‑CTT‑GCCTCCAAAAAAAAAAAAAAATCCCC‑ATTTTTTTTTTTAACAAAAAAAAAAAAACTTAAAAAAAATAATGTTATTTTTTAAAAAAAAATAAAAAGCATTAATTAAATATTaaaaaaaaaa > 2:332257/1‑150 (MQ=255) agagGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAataata < 2:141078/150‑1 (MQ=255) gagGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGa < 2:161814/151‑1 (MQ=255) cGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaa < 1:787591/151‑1 (MQ=255) ttATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaa < 1:944930/135‑1 (MQ=255) ttATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaa > 2:944846/1‑135 (MQ=255) aTGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt < 2:890323/151‑1 (MQ=255) gTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCt > 2:703319/1‑151 (MQ=255) aaaCCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTa > 2:575520/1‑150 (MQ=255) aaCCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc > 2:793782/1‑151 (MQ=255) | GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGTAAAACCACTCA‑TTA‑GCCTCAAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAC > NC_000913/2903542‑2903840 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |