| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,355,457 | 0 | A | C | 49.1% | 85.5 / 20.3 | 60 | T245P (ACC→CCC) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (28/2); new base C (0/29); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.39e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGC > NC_000913/3355310‑3355603 | ccGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCg < 2:955713/151‑1 (MQ=255)ccGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCg > 1:931278/1‑151 (MQ=255) tttATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAAcc < 2:776367/150‑1 (MQ=255) tttATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCg > 1:734955/1‑151 (MQ=255) accTTGCGGCCCGGCGTCTGGATGCGCCCATTTGCCTGTCCCCCCAGCGCTTCCCCTCAAACCCCTC‑CCCGCGCTGGCCGCTGGCCCACCCGTTCCGCTATCTGGCGCATAAGGGTGAAATCACCCCCATCCCCGGTATCCGCCAATGGgc < 2:826144/149‑1 (MQ=255) ggACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGgcgcgtgcg > 2:555205/1‑151 (MQ=255) caggCGTCTGGAACCGGCTTTTTGCCTTTCCCCCCAGCGCTTCTCCCCAAACCCCGT‑CCCGCGCTGGCCGCTGGCGCACCCTTCCCGCTATCTGGCGCAAAACGGTGAAAAAACCACCATCCCCGGTAACCGCCAATGGgcg < 2:997406/139‑1 (MQ=255) tGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTAc > 2:812936/1‑151 (MQ=255) gCGTCGGGAATCGCCCTTTTGCCTGTCCCCCCAGCGTTTCTCCACTAACCCCGT‑CCCGCGCTGGCCGCGGGCGCAACCGTTCCGCTATCTGGCGCAAAACGGGGAAATAACCCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTa < 1:698133/149‑1 (MQ=255) gTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACtt > 1:434948/1‑150 (MQ=255) cTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTtat > 1:625451/1‑150 (MQ=255) cTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTtat > 1:114524/1‑150 (MQ=255) gaaaTCGCCCTTTTCCCTGTCCCCCCGGCGTTTCCCCACTAACCCCGT‑CCCGCGCGGCCCGCGGGCGCACCCGTCCCGCTATCTGGCGCATAGCGGTAAAATCAACACCACCCCGGGTAACCGCCAATGGGCGCGTGCGCGTACTtata < 2:673891/147‑1 (MQ=255) accGGCCTTTTGCCTTTTCCACCAGCGCTTCCCCACAAACCCCGT‑CCCGCGCGGGCCGCTGGCGCACCCGTTCCGCTATCTGGCGCATAACGGTAAAATCAACACCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTc < 1:508046/149‑1 (MQ=255) ggCCTTTTGCCTGTTCCCCCAGCGCTTCCCCACTAACCCCGT‑ACCGCGCGGGCCGCTGGCGCACCCGTTCCGCTATCTGGCGCATAACGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAg < 1:778674/151‑1 (MQ=255) ctttttGCCTTTCCCCCCAGCGCTCCCCCACAAAC‑CCGTCCCCGCGCGGGCGGTTGGCACACCCGTGCCGCTATCGGGCGCATAAGGGGGAAATAAACACCTCCGCAGGTAACCGCCAATGGGCGCGTGCGCGTACTTATaa < 1:627690/139‑1 (MQ=255) cctttgtcgtgccccacgaGCGTTTCCCCACAACCCCCGA‑ACCGCGCAGGCCATTGGCAGACCCGTCCCCCTATCTGGCGCAAAACGGTGAAATCAACGCCGCCCCCGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGac < 1:478910/138‑1 (MQ=255) ttttGCCTTTCCCCCCGGCGCTTCTCCACTAACCCCGT‑CCCGCGCGGGCCGCGGGCGCACCCGTCCGGCTGTCTGCCGCAAAGCGGTGAAATCAACCCCCTCCCCGTAAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGa < 1:335990/147‑1 (MQ=255) cTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCtt > 1:50569/1‑150 (MQ=255) gTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATc > 1:137109/1‑151 (MQ=255) cccccgccgcTTCTCCACTAACCCCGT‑CCCGCGCTGCCCGCTGGCCCACCCGTCCCGCTATCTGGCGCATACCGGTAAAATCACCACCTTCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCt < 2:138469/137‑1 (MQ=255) ccaccaGCGCTTCGCCACTAACCCCGT‑CCCGCGCTGGCGGCGGGCCCACCCGTTCCGGTATCGGGCGCATAAGGGTGAAATCAACACCACCCCGGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATccc < 1:393254/150‑1 (MQ=255) caccaGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTg > 1:75264/1‑151 (MQ=255) ccaGCCCTTCCCCACTACCCCCGC‑CCCGCGCTGGCGGCTGGCGCAACCTTTCCGCTTTCTGGGGCAAACGGGTAAAATCAACACCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGAc < 1:794452/151‑1 (MQ=255) caGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGAcc > 1:623519/1‑151 (MQ=255) aGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCt > 1:320684/1‑151 (MQ=255) cgcTTCTCCACTAACCCCGC‑CCCGCGCTGGCCGCTGGCGCACCCGTTCCGCTATCTGGCGCATAGCGGTGAAATCAACACCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTg < 1:59140/150‑1 (MQ=255) cgcTTCCCCACTACCCCCGT‑CCCGCGCTGCCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTAAAATCAACCCCACCCCGGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCt < 1:277632/149‑1 (MQ=255) ttCCCCACAACCCCCGT‑ACCGCGCTGGCCGCTGGCCCACCCTTCCCTCTATCTGGCGCAAAAGGGAAAAATCAACCCCACCCCCGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCt < 2:448366/146‑1 (MQ=255) ttCCCCACAAACCCCGC‑CCCGCGCGGCCCGCTGGCGCACCCGTCCCCCTTTTTGGCGCATACGGGAAAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCa < 2:468334/149‑1 (MQ=255) tctcCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCacg > 1:866213/1‑150 (MQ=255) ccccACTAACCCCGT‑CCCGCGCTGGCCGCTGGCGCCCCCGTCCCGCTATCTGGCGCATAACGGTGAAATAAACCCCATCCCGGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCacgac < 1:45248/149‑1 (MQ=255) ccacaacccccGT‑CCCGCGCTGGCCGCTGGCGCCCCCGTCCCGCTATCTGGCGCATAACGGGAAAATCAACACCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCacgac < 2:2901/140‑1 (MQ=255) aaCACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCAc > 1:545570/1‑151 (MQ=255) cacCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCg > 2:541713/1‑151 (MQ=255) acCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGt > 1:589376/1‑151 (MQ=255) ccGGGCGGGCCGCGGGCGCACCCTTTCCGCTTTCTGGCGCAAAGGGGTAAAATCACCACCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCa < 2:548802/151‑1 (MQ=255) gcgcTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAAc > 2:629511/1‑151 (MQ=255) ggccgctggcGAACCCGTCCCGCTATCGGGCGCATAGGGGTAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGaaa < 1:860629/150‑1 (MQ=255) ccgctggcGCACCCTTCCCGTTTTCGGGCGCAAAGGGGTGAAATAAACACCCCCCCCGGTACCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGaa < 2:45852/147‑1 (MQ=255) ccgctggcGCACCCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCg < 1:329292/151‑1 (MQ=255) cgcACCCGTTCCTCTATCTGGCGCATACGGGTAAAATCAACCCCATCCCCGGTATCCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGa < 2:355904/151‑1 (MQ=255) cccaccctttCCGCTTTTGGGCGCATAGGGGAAAAATCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTg < 2:297607/142‑1 (MQ=255) cAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACt > 2:233360/1‑151 (MQ=255) acccGTTCCGCTATCGGGCGCATAAGGGTAAAATCAACACCACCCCCGTTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACt < 1:240673/148‑1 (MQ=255) aaCCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTc > 1:37740/1‑151 (MQ=255) cccttccccctttCTGGCGAATAACGGAAAAACCACCACCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTcc < 2:781610/139‑1 (MQ=255) gTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGt > 2:106724/1‑151 (MQ=255) ccGCTTTCGGGCGCATAACGGGAAAACCAACCCCACCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGtt < 2:614251/149‑1 (MQ=255) tATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATgg > 1:842462/1‑151 (MQ=255) aTCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATgg > 2:761433/1‑150 (MQ=255) ggCGCATAACGGAAAAATCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAAc < 2:83011/151‑1 (MQ=255) aaaaCGGTGAAACCAACCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCt < 2:734876/149‑1 (MQ=255) gTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAAct > 2:437083/1‑151 (MQ=255) tGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAActg > 1:653889/1‑151 (MQ=255) aaCCCCATCCCCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTgg < 1:312352/151‑1 (MQ=255) cacCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGc > 1:23966/1‑150 (MQ=255) acCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGc > 2:71972/1‑149 (MQ=255) acCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGc > 2:287156/1‑149 (MQ=255) cATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGc > 2:659346/1‑151 (MQ=255) | CCGCGCTTTTATCTGGATCTTGCGGACCTGCGTCTGGAATCGGCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGT‑ACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGC > NC_000913/3355310‑3355603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |