Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,556	A→G	intergenic (+524/‑78)	cra → / → mraZ	transcriptional repressor‑activator for carbon metabolism/RsmH methytransferase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,556	0	A	G	100.0%	273.6 / NA	75	intergenic (+524/‑78)	cra/mraZ	transcriptional repressor‑activator for carbon metabolism/RsmH methytransferase inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/38);  total (37/38)

ATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTA  >  NC_000913/89408‑89698                                                                                                                                                     |                                                                                                                                               aTTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCa                                                                                                                                              <  1:959942/151‑1 (MQ=255)       aaaCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCttt                                                                                                                                        >  1:975407/1‑151 (MQ=255)       aaaCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCttt                                                                                                                                        >  1:468781/1‑151 (MQ=255)         aCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTc                                                                                                                                      <  2:842293/151‑1 (MQ=255)                 gaCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTc                                                                                                                              <  2:267133/151‑1 (MQ=255)                  aCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTcc                                                                                                                             >  1:940462/1‑151 (MQ=255)                     aTGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTa                                                                                                                          <  2:202168/151‑1 (MQ=255)                      tGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTcc                                                                                                                             >  2:325128/1‑147 (MQ=255)                        aGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCTAGCTTTTCCTCAGCTCCGTaa                                                                                                                         >  1:799576/1‑149 (MQ=255)                        aGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTaaa                                                                                                                        >  2:333386/1‑150 (MQ=255)                         gggATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACt                                                                                                                      <  1:357755/151‑1 (MQ=255)                           gATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTcc                                                                                                                    >  2:440282/1‑151 (MQ=255)                               ttttCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTTCTCAGCTCCGTAAACTCCttt                                                                                                                 >  2:97696/1‑150 (MQ=255)                                 ttCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCttt                                                                                                                 >  1:846326/1‑148 (MQ=255)                                 ttCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCa                                                                                                               <  2:287672/150‑1 (MQ=255)                                  tCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAg                                                                                                              <  2:63064/150‑1 (MQ=255)                                  tCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAg                                                                                                              <  2:644396/150‑1 (MQ=255)                                   cTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTg                                                                                                            >  1:646363/1‑151 (MQ=255)                                     tACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTggg                                                                                                          >  2:499524/1‑151 (MQ=255)                                       cAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCCCCGTAAACTCCTTTCAGTggg                                                                                                          >  2:764308/1‑149 (MQ=255)                                        aGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTggg                                                                                                          >  2:372688/1‑148 (MQ=255)                                         gCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTTAGTGGGaaa                                                                                                       >  2:67257/1‑150 (MQ=255)                                          cTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTc                                                                                                                                   >  1:615055/1‑121 (MQ=255)                                          cTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTc                                                                                                                                   <  2:614993/121‑1 (MQ=255)                                            aTTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATtg                                                                                                    >  1:743744/1‑150 (MQ=255)                                             ttCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATtgtg                                                                                                  >  1:246524/1‑151 (MQ=255)                                              tCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTgg                                                                                                 >  2:766404/1‑151 (MQ=255)                                               cATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTgg                                                                                                 <  1:172404/150‑1 (MQ=255)                                                    cGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCaaa                                                                                           <  1:300807/151‑1 (MQ=255)                                                    cGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGATCCCTAAACTCCTTTCAGTGGGAAATTGTGGGGCaa                                                                                            >  2:594331/1‑150 (MQ=255)                                                      ttAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAg                                                                                          >  1:476080/1‑150 (MQ=255)                                                      ttAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAg                                                                                          >  2:63662/1‑150 (MQ=255)                                                          tttGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGGAAGCTTTTTCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGGAAAGTggg                                                                                      >  1:989688/1‑150 (MQ=255)                                                                tCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATaa                                                                                 <  1:896183/149‑1 (MQ=255)                                                                tCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAg                                                                                <  1:737048/150‑1 (MQ=255)                                                                 cGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAggg                                                                              <  1:9977/151‑1 (MQ=255)                                                                  gCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAgggg                                                                             >  1:495825/1‑151 (MQ=255)                                                                   cACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAgggg                                                                             >  1:22933/1‑150 (MQ=255)                                                                    aCGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTg                                                                           >  1:988923/1‑151 (MQ=255)                                                                     cGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTg                                                                           >  1:264727/1‑150 (MQ=255)                                                                       ttGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAACTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGAGGGAATAAGGGGGGAgg                                                                        >  2:614614/1‑151 (MQ=255)                                                                                aaaTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGt                                                               <  1:348510/151‑1 (MQ=255)                                                                                    aaaCAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTcc                                                            <  2:312609/150‑1 (MQ=255)                                                                                         acGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAg                                                      <  2:587776/151‑1 (MQ=255)                                                                                               atatTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACg                                                 >  1:258493/1‑150 (MQ=255)                                                                                               atatTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAACTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGTGGGGATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAAc                                                  >  2:388736/1‑149 (MQ=255)                                                                                                 atTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGtt                                               <  2:258459/150‑1 (MQ=255)                                                                                                   tAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAg                                             >  1:21221/1‑150 (MQ=255)                                                                                                   tAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAg                                             <  2:277925/150‑1 (MQ=255)                                                                                                    aGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGt                                            <  2:495182/150‑1 (MQ=255)                                                                                                     gCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCa                                          <  1:889696/151‑1 (MQ=255)                                                                                                      ccGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCaa                                         <  2:408759/151‑1 (MQ=255)                                                                                                        gTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAAtc                                       <  1:228431/151‑1 (MQ=255)                                                                                                        gTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGGGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAAt                                        >  1:469665/1‑150 (MQ=255)                                                                                                          aaaCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAAtctc                                     <  1:557398/151‑1 (MQ=255)                                                                                                           aaCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTGCCGCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAAtct                                      <  1:560829/149‑1 (MQ=255)                                                                                                            aCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGa                                   <  2:717135/151‑1 (MQ=255)                                                                                                             cATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGAc                                  <  2:975322/151‑1 (MQ=255)                                                                                                                cGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAg                                <  2:719345/150‑1 (MQ=255)                                                                                                                cGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTGTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGc                               <  2:608900/151‑1 (MQ=255)                                                                                                                   gTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCaaa                            <  1:732404/151‑1 (MQ=255)                                                                                                                   gTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGACAATCTCGACAGCaaa                            <  1:998544/151‑1 (MQ=255)                                                                                                                     tttttACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGGTCCGGGGAGCAACGTTAGTCAATCTCGACAGCaaa                            >  1:531100/1‑149 (MQ=255)                                                                                                                         tACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGgcg                       <  2:498058/150‑1 (MQ=255)                                                                                                                         tACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGgcg                       <  1:431019/150‑1 (MQ=255)                                                                                                                         tACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGgcg                       <  2:353846/150‑1 (MQ=255)                                                                                                                         tACCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGgcg                       <  1:29091/150‑1 (MQ=255)                                                                                                                          aCCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGGAATTGTGGGGCAAAGAGGGGATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCt                     >  1:745858/1‑151 (MQ=255)                                                                                                                          aCCTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCt                     >  1:283366/1‑151 (MQ=255)                                                                                                                           ccTCGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCtt                    <  1:755723/151‑1 (MQ=255)                                                                                                                              cGGTATGCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTa                   <  2:743668/149‑1 (MQ=255)                                                                                                                                    gCCTTGTGACTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGcc           >  1:771745/1‑151 (MQ=255)                                                                                                                                     ccTTGTGACTGGCTTGGCCAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGGTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGcc           >  2:17501/1‑150 (MQ=255)                                                                                                                                            aCTGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGt    <  2:122567/150‑1 (MQ=255)                                                                                                                                              tGGCTTGGCAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTa  >  2:370671/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                               ATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGCATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTA  >  NC_000913/89408‑89698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑