Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,831,451	A→G	E332G (GAA→GGA)	yicH →	putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,831,451	0	A	G	100.0%	208.3 / NA	57	E332G (GAA→GGA)	yicH	putative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/39);  total (18/39)

AAGATGACTGGCAGACACAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGAAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGAAACGACACCCGGTTGGTTA  >  NC_000913/3831307‑3831593                                                                                                                                                 |                                                                                                                                               aaGATGACTGGCAGACACAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAggggg                                                                                                                                          >  1:780420/1‑151 (MQ=255)  aGATGACTGGCAGACACAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAggggg                                                                                                                                          >  2:428584/1‑150 (MQ=255)   gATGACTGGCAGACACAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTa                                                                                                                                        <  1:408665/151‑1 (MQ=255)   gATGACTGGCAGACACAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTa                                                                                                                                        <  1:414370/151‑1 (MQ=255)       aCTGGCAGACACAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGGTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATg                                                                                                                                      >  2:38232/1‑149 (MQ=255)               acacAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGt                                                                                                                            <  1:243007/151‑1 (MQ=255)                 acaGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCa                                                                                                                          <  1:791808/151‑1 (MQ=255)                 acaGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCa                                                                                                                          <  2:157806/151‑1 (MQ=255)                    ggAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGTTGGGGAGGGGGTATGGTCAGAACGTCAgg                                                                                                                        <  1:576620/150‑1 (MQ=255)                     gAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGa                                                                                                                      <  1:112249/151‑1 (MQ=255)                       aGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAAc                                                                                                                    <  1:869617/151‑1 (MQ=255)                        ggCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACt                                                                                                                   <  2:206117/151‑1 (MQ=255)                          cAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTgg                                                                                                                 <  1:744426/151‑1 (MQ=255)                                gTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGt                                                                                                           <  1:953853/151‑1 (MQ=255)                                     tGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACg                                                                                                       <  2:260386/150‑1 (MQ=255)                                      gAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACgg                                                                                                      <  1:427860/150‑1 (MQ=255)                                       aaCGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGa                                                                                                    <  2:332994/151‑1 (MQ=255)                                          gCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGaaaa                                                                                                 <  2:30791/151‑1 (MQ=255)                                           cTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAAc                                                                                                >  1:256727/1‑151 (MQ=255)                                             aGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCTCCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACg                                                                                               <  1:815785/150‑1 (MQ=255)                                               cGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGtt                                                                                             <  2:244816/150‑1 (MQ=255)                                                     cATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCtt                                                                                      <  1:928890/151‑1 (MQ=255)                                                      aTTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTtg                                                                                     <  2:936660/151‑1 (MQ=255)                                                        ttATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTtga                                                                                    <  1:709379/150‑1 (MQ=255)                                                               tCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCg                                                                             <  1:522578/150‑1 (MQ=255)                                                                cGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCgg                                                                            <  2:814313/150‑1 (MQ=255)                                                                   tGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAAt                                                                        >  2:596393/1‑151 (MQ=255)                                                                    gCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAAtt                                                                       <  1:571219/151‑1 (MQ=255)                                                                         tatttGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCgg                                                                  >  1:887304/1‑151 (MQ=255)                                                                          atttGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCggg                                                                 <  1:87768/151‑1 (MQ=255)                                                                               aCCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTgg                                                             >  2:341231/1‑150 (MQ=255)                                                                                      tataAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATAcc                                                     >  1:297247/1‑151 (MQ=255)                                                                                         aaaCACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCtt                                                   <  2:353189/150‑1 (MQ=255)                                                                                            cacGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCg                                               >  2:734671/1‑151 (MQ=255)                                                                                            cacGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCg                                               <  1:595894/151‑1 (MQ=255)                                                                                            cacGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCg                                               <  2:799444/151‑1 (MQ=255)                                                                                              cGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGaa                                             <  1:998675/151‑1 (MQ=255)                                                                                                gAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGaaaa                                           <  1:535148/151‑1 (MQ=255)                                                                                                      tCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGc                                     >  1:465292/1‑151 (MQ=255)                                                                                                       ccccGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGc                                     <  1:576484/150‑1 (MQ=255)                                                                                                          cGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACa                                 <  1:883290/151‑1 (MQ=255)                                                                                                           gCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAAc                                  >  1:64042/1‑149 (MQ=255)                                                                                                            cAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGt                               <  1:177638/151‑1 (MQ=255)                                                                                                              gggCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTtg                             >  1:655267/1‑151 (MQ=255)                                                                                                               ggCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTtgt                            <  2:389790/151‑1 (MQ=255)                                                                                                                gCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTtgtg                           >  1:517044/1‑151 (MQ=255)                                                                                                                 cGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGtgg                          >  2:729358/1‑151 (MQ=255)                                                                                                                 cGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGtgg                          >  1:832442/1‑151 (MQ=255)                                                                                                                  gTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGtgga                         <  2:832366/151‑1 (MQ=255)                                                                                                                  gTAGCGCTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGtgg                          >  2:753529/1‑150 (MQ=255)                                                                                                                      cgcTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGtggatgga                     <  1:821364/151‑1 (MQ=255)                                                                                                                        cTGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGaa                    <  1:150972/150‑1 (MQ=255)                                                                                                                         tGCGCCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGAAAc                  >  1:349294/1‑151 (MQ=255)                                                                                                                          gcgcCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGaaa                   <  1:179545/149‑1 (MQ=255)                                                                                                                           cgcCAGTTCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGAAACGa                <  2:780339/151‑1 (MQ=255)                                                                                                                                 ttCACCAGCCGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGAAACGACACCCg          >  1:306972/1‑151 (MQ=255)                                                                                                                                         ccGCTGGGGAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGAAACGACACCCGGTTGGTTa  <  1:560926/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               AAGATGACTGGCAGACACAGGAAGGCAAACTGTCGATGAACGCTAGCGAGTTCATTTATGGTTCGCTGCATTTATTTGACCCGATTATAAACACGGAATTTTCCCCGCAGGGCGTAGCGCTGCGCCAGTTCACCAGCCGCTGGGAAGGGGGTATGGTCAGAACGTCAGGGAACTGGCTGCGTGACGGGAAAACGTTGATCCTTGATGATGCGGCAATTGCCGGGCTGGAATATACCTTGCCGAAAAACTGGCAACAGTTGTGGATGGAAACGACACCCGGTTGGTTA  >  NC_000913/3831307‑3831593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑