Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,106,405	T→C	intergenic (+85/‑64)	cpxP → / → fieF	inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,106,405	0	T	C	100.0%	176.5 / NA	47	intergenic (+85/‑64)	cpxP/fieF	inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

CGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAAAA  >  NC_000913/4106259‑4106550                                                                                                                                                   |                                                                                                                                                  cgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCtt                                                                                                                                               <  2:876716/151‑1 (MQ=255)     aTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATg                                                                                                                                           <  2:390209/151‑1 (MQ=255)       ggCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGc                                                                                                                                          <  1:70358/150‑1 (MQ=255)        gCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCAt                                                                                                                                        <  1:6583/151‑1 (MQ=255)          aaaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTg                                                                                                                                      >  1:465705/1‑151 (MQ=255)              aaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGc                                                                                                                                     >  2:227255/1‑148 (MQ=255)               aGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAgat                                                                                                                                 <  1:84881/151‑1 (MQ=255)                   cATCGCTGAAGCTATTGAGTAGTAGCAACCCACGTTCCCAGAAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCGCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGAtt                                                                                                                             >  2:157218/1‑151 (MQ=255)                         tGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCtt                                                                                                                        <  2:5836/150‑1 (MQ=255)                          gAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTcc                                                                                                                      >  2:97721/1‑151 (MQ=255)                             gCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGtt                                                                                                                   <  2:410512/151‑1 (MQ=255)                              cTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGtt                                                                                                                   >  2:537827/1‑150 (MQ=255)                                    agtagtAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACt                                                                                                              >  1:970192/1‑149 (MQ=255)                                     gtagtaGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGc                                                                                                           <  1:928258/151‑1 (MQ=255)                                        gtagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTc                                                                                                        >  1:128440/1‑151 (MQ=255)                                                tCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGAt                                                                                                 >  1:355264/1‑150 (MQ=255)                                                 cACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGAt                                                                                                 <  2:106756/149‑1 (MQ=255)                                                       cccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCggg                                                                                           >  1:134156/1‑149 (MQ=255)                                                       cccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGa                                                                                          <  2:407843/150‑1 (MQ=255)                                                       cccAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGa                                                                                          <  2:225054/150‑1 (MQ=255)                                                             agtaAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTAtt                                                                                     >  1:330283/1‑149 (MQ=255)                                                              gtaAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTAt                                                                                  <  2:2874/151‑1 (MQ=255)                                                               taAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTAt                                                                                  >  2:557002/1‑150 (MQ=255)                                                               taAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATg                                                                                 >  2:855086/1‑151 (MQ=255)                                                                 aaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGa                                                                                >  1:302860/1‑150 (MQ=255)                                                                         tttCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCtt                                                                       >  1:934698/1‑151 (MQ=255)                                                                            ccTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTAt                                                                     <  2:465434/150‑1 (MQ=255)                                                                            ccTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATg                                                                    <  2:417208/151‑1 (MQ=255)                                                                                                      tCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGAt                                           <  1:684274/150‑1 (MQ=255)                                                                                                         cccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct                                       >  2:987910/1‑151 (MQ=255)                                                                                                             caTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGa                                   <  1:97737/151‑1 (MQ=255)                                                                                                              aTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGAc                                  >  2:469367/1‑151 (MQ=255)                                                                                                                   gtgGGGGTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCg                              >  1:474208/1‑150 (MQ=255)                                                                                                                     gggggTTTTTTTTATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATg                           <  1:501891/151‑1 (MQ=255)                                                                                                                          gtttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTc                      <  2:279265/150‑1 (MQ=255)                                                                                                                           tttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTc                      <  1:537882/150‑1 (MQ=255)                                                                                                                           tttttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTc                      <  2:795208/150‑1 (MQ=255)                                                                                                                              ttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgctg                  >  1:378859/1‑151 (MQ=255)                                                                                                                              ttttATCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCgct                   <  2:838538/150‑1 (MQ=255)                                                                                                                                  aTCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTAt              >  1:553764/1‑151 (MQ=255)                                                                                                                                   tCCTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTAtt             >  2:54464/1‑151 (MQ=255)                                                                                                                                    ccTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTg            <  2:128429/151‑1 (MQ=255)                                                                                                                                    ccTCAATTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTg            >  1:689106/1‑151 (MQ=255)                                                                                                                                        aaTTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTg         >  2:80227/1‑150 (MQ=255)                                                                                                                                        aaTTTGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTg         >  1:673431/1‑150 (MQ=255)                                                                                                                                           ttGCCTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGAtt      >  1:309788/1‑150 (MQ=255)                                                                                                                                              ccTGCCGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTaaaa  <  2:122505/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAAAA  >  NC_000913/4106259‑4106550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑