Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,114,622	A→G	S320P (TCC→CCC)	glpX ←	fructose 1,6‑bisphosphatase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,114,622	0	A	G	100.0%	213.3 / NA	55	S320P (TCC→CCC)	glpX	fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/30);  total (25/30)

TTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCATA  >  NC_000913/4114474‑4114767                                                                                                                                                     |                                                                                                                                                  tttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCt                                                                                                                                                 >  1:562002/1‑151 (MQ=255)  ttttCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTg                                                                                                                                                >  1:884138/1‑151 (MQ=255)        ccTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAAt                                                                                                                                             <  2:28790/148‑1 (MQ=255)        ccTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATgcg                                                                                                                                          <  2:127509/151‑1 (MQ=255)        ccTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATg                                                                                                                                            <  2:338230/149‑1 (MQ=255)                   tgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGc                                                                                                                               >  2:265100/1‑151 (MQ=255)                        tGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTg                                                                                                                            <  1:234186/149‑1 (MQ=255)                         gCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACt                                                                                                                         <  1:390827/151‑1 (MQ=255)                          ccTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACtt                                                                                                                        <  2:679964/151‑1 (MQ=255)                           cTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTg                                                                                                                       >  2:456441/1‑151 (MQ=255)                              aTCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCcg                                                                                                                    >  1:774154/1‑151 (MQ=255)                              aTCTTCGTTCTCCGAACAAAAAAATTCCAGTCACGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGcc                                                                                                                     <  1:924455/150‑1 (MQ=255)                                 ttCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCgg                                                                                                                 >  1:922571/1‑151 (MQ=255)                                   cGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGAt                                                                                                               <  2:926596/151‑1 (MQ=255)                                    gTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATc                                                                                                              >  1:148987/1‑151 (MQ=255)                                         ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagc                                                                                                           >  1:630040/1‑149 (MQ=255)                                              cAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGttt                                                                                                    >  2:16507/1‑151 (MQ=255)                                                             cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGatat                                                                                     <  2:10531/151‑1 (MQ=255)                                                               cGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTg                                                                                   <  2:209788/151‑1 (MQ=255)                                                                 aaGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGcc                                                                                 <  1:456493/151‑1 (MQ=255)                                                                     aCTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCtttt                                                                              >  1:405276/1‑150 (MQ=255)                                                                      cTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGc                                                                            >  2:86925/1‑151 (MQ=255)                                                                       tGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCg                                                                           >  2:930328/1‑151 (MQ=255)                                                                        ggAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGc                                                                            <  1:374339/149‑1 (MQ=255)                                                                            ggCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTaa                                                                      <  1:16510/151‑1 (MQ=255)                                                                             gCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAAt                                                                     <  1:542773/151‑1 (MQ=255)                                                                                   tCGATCAAATCAATCAGAGGATGTGCACCTGCATTACCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCtt                                                                <  2:964243/150‑1 (MQ=255)                                                                                     gATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGAAAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTcc                                                              >  1:615364/1‑150 (MQ=255)                                                                                      atcaaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcag                                                            <  2:729396/151‑1 (MQ=255)                                                                                        caaatcaaTCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCcagc                                                           <  1:892516/150‑1 (MQ=255)                                                                                           atcaatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGAt                                                       <  1:514154/151‑1 (MQ=255)                                                                                           atcaatcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGAt                                                       <  2:732081/151‑1 (MQ=255)                                                                                               atcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGcc                                                   >  2:296948/1‑151 (MQ=255)                                                                                                tcaGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCt                                                  >  1:95183/1‑151 (MQ=255)                                                                                                 caGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCtt                                                 <  1:660714/151‑1 (MQ=255)                                                                                                     ggATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGt                                             <  2:652785/151‑1 (MQ=255)                                                                                                      gATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTa                                            <  2:550525/151‑1 (MQ=255)                                                                                                       aTGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTaa                                           <  1:950413/151‑1 (MQ=255)                                                                                                           gCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATAcc                                       <  1:484824/151‑1 (MQ=255)                                                                                                                tGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTgg                                  >  1:28791/1‑151 (MQ=255)                                                                                                                 gCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTg                                   <  2:572255/149‑1 (MQ=255)                                                                                                                       ccGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAgaga                            >  1:959140/1‑150 (MQ=255)                                                                                                                       ccGGGTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGagaa                           <  2:845816/151‑1 (MQ=255)                                                                                                                          ggTCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAgaga                            >  1:137314/1‑147 (MQ=255)                                                                                                                            tCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATg                       >  2:150665/1‑150 (MQ=255)                                                                                                                            tCTTTGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATg                       >  2:57670/1‑150 (MQ=255)                                                                                                                              tttGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACg                    >  2:328609/1‑151 (MQ=255)                                                                                                                               ttGCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGt                   >  2:505427/1‑151 (MQ=255)                                                                                                                               ttGCGACCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGAc                     <  2:293442/149‑1 (MQ=255)                                                                                                                                 gCGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGtt                  >  2:416078/1‑150 (MQ=255)                                                                                                                                  cGATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTAt                <  1:215009/151‑1 (MQ=255)                                                                                                                                   gATCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATc               >  1:168178/1‑151 (MQ=255)                                                                                                                                    aTCCAGATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATTCCGGTGGCAGAGAAGATGACGTTATCg              <  1:642959/151‑1 (MQ=255)                                                                                                                                         gATAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcg         <  2:177642/151‑1 (MQ=255)                                                                                                                                          aTAGTGGATGGGCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTgcgc        >  1:912363/1‑151 (MQ=255)                                                                                                                                                gatgggCTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCata  <  2:774071/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATATTGCCTTTGCGGCTAATGCCTTCCAGCAGATCGCCTTTGGTAATACCGGTGGCAGAGAAGATGACGTTATCGCTGCGCGCCATA  >  NC_000913/4114474‑4114767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑