Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,180,463	T→C	A156A (GCT→GCC)	rplJ →	50S ribosomal subunit protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,180,463	0	T	C	100.0%	271.6 / NA	72	A156A (GCT→GCC)	rplJ	50S ribosomal subunit protein L10
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/45);  total (27/45)

AGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCT  >  NC_000913/4180315‑4180607                                                                                                                                                     |                                                                                                                                                 aGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGgccgc                                                                                                                                                >  1:286619/1‑151 (MQ=255)  ggTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGgccgct                                                                                                                                               <  2:4511/151‑1 (MQ=255)   gTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGccgctg                                                                                                                                              <  1:580985/151‑1 (MQ=255)       aaGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTAcg                                                                                                                                          >  2:375347/1‑151 (MQ=255)          ccGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTAcg                                                                                                                                          >  1:9637/1‑148 (MQ=255)            gCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATg                                                                                                                                     <  2:970438/151‑1 (MQ=255)            gCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATg                                                                                                                                     >  1:157332/1‑151 (MQ=255)               gCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGa                                                                                                                                  <  2:990909/151‑1 (MQ=255)                   ttGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGaaa                                                                                                                                <  2:116162/149‑1 (MQ=255)                   ttGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGaa                                                                                                                                 >  2:377663/1‑148 (MQ=255)                    tGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaag                                                                                                                               <  2:52357/149‑1 (MQ=255)                     gAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAaagaa                                                                                                                             <  1:261524/150‑1 (MQ=255)                         gTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAgctgc                                                                                                                        <  2:284580/151‑1 (MQ=255)                          tGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAgctgct                                                                                                                       >  2:662834/1‑151 (MQ=255)                           gAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAgctgct                                                                                                                       <  1:836991/150‑1 (MQ=255)                           gAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCtt                                                                                                                      >  2:252118/1‑151 (MQ=255)                           gAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCtt                                                                                                                      <  1:765360/151‑1 (MQ=255)                           gAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCtt                                                                                                                      >  1:614323/1‑151 (MQ=255)                             gCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTa                                                                                                                     <  2:860089/150‑1 (MQ=255)                               tGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATc                                                                                                                  >  1:150727/1‑151 (MQ=255)                                gATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATc                                                                                                                  <  2:95031/150‑1 (MQ=255)                                gATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCg                                                                                                                 >  1:852490/1‑151 (MQ=255)                                   cccGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGc                                                                                                                >  1:761886/1‑149 (MQ=255)                                   cccGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCa                                                                                                               <  1:510434/150‑1 (MQ=255)                                    ccGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGt                                                                                                             >  1:256548/1‑151 (MQ=255)                                      ggCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGtt                                                                                                            >  2:942121/1‑150 (MQ=255)                                         gTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCt                                                                                                        <  1:237933/151‑1 (MQ=255)                                           ctcAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCttt                                                                                                      >  2:566133/1‑151 (MQ=255)                                              aGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCttttt                                                                                                    <  2:558126/150‑1 (MQ=255)                                               gATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTaa                                                                                                  <  2:568017/151‑1 (MQ=255)                                               gATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTaa                                                                                                  <  2:256248/151‑1 (MQ=255)                                                  cGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACg                                                                                                <  2:432298/150‑1 (MQ=255)                                                     ccGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCAtt                                                                                            <  1:738226/151‑1 (MQ=255)                                                     ccGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCAtt                                                                                            <  1:759843/151‑1 (MQ=255)                                                       gCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCg                                                                                          >  1:88316/1‑151 (MQ=255)                                                       gCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCg                                                                                          <  1:67617/151‑1 (MQ=255)                                                           ggCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCtt                                                                                       <  2:705752/150‑1 (MQ=255)                                                           ggCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCtt                                                                                       <  2:855013/150‑1 (MQ=255)                                                            gCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTAc                                                                                     <  1:635390/151‑1 (MQ=255)                                                                  ctGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGtat                                                                                 <  1:263751/149‑1 (MQ=255)                                                                    gCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATaa                                                                               <  1:332990/149‑1 (MQ=255)                                                                    gCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAAc                                                                             >  2:919553/1‑151 (MQ=255)                                                                      cGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACtt                                                                           <  2:708964/151‑1 (MQ=255)                                                                           tACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCt                                                                      >  2:404348/1‑151 (MQ=255)                                                                            aCGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTg                                                                     <  1:470971/151‑1 (MQ=255)                                                                              gaagaaGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGat                                                                   >  2:544522/1‑151 (MQ=255)                                                                               aagaagCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGata                                                                  <  1:260138/151‑1 (MQ=255)                                                                               aagaagCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGat                                                                   >  1:302982/1‑150 (MQ=255)                                                                                agaagCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGatat                                                                 <  1:217155/151‑1 (MQ=255)                                                                                    gCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAg                                                             <  2:133505/151‑1 (MQ=255)                                                                                      aaTTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGa                                                           <  2:74973/151‑1 (MQ=255)                                                                                          gCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAATCTTATTCTGATATTCAGGAACa                                                        <  1:632544/150‑1 (MQ=255)                                                                                          gCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACa                                                        >  2:850983/1‑150 (MQ=255)                                                                                            aCGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAAtt                                                     >  2:86281/1‑151 (MQ=255)                                                                                              gCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTa                                                   <  1:782697/151‑1 (MQ=255)                                                                                              gCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTa                                                   <  2:768463/151‑1 (MQ=255)                                                                                              gCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTa                                                   <  2:81482/151‑1 (MQ=255)                                                                                                  gATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTaaa                                                 <  1:537455/149‑1 (MQ=255)                                                                                                  gATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAAt                                                <  1:557231/150‑1 (MQ=255)                                                                                                       cAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTAt                                          >  2:290050/1‑151 (MQ=255)                                                                                                           cATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACt                                      <  1:847205/151‑1 (MQ=255)                                                                                                                  gaagCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTaa                                    >  1:886375/1‑146 (MQ=255)                                                                                                                    agCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCaa                             <  1:784280/151‑1 (MQ=255)                                                                                                                    agCTTCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCa                              >  2:481212/1‑150 (MQ=255)                                                                                                                        tCGGCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAAt                           <  2:571447/149‑1 (MQ=255)                                                                                                                          ggCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCAtt                       <  1:662905/151‑1 (MQ=255)                                                                                                                           gCTGGCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTg                      <  1:102292/151‑1 (MQ=255)                                                                                                                              ggCAAACTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAg                   <  1:186097/151‑1 (MQ=255)                                                                                                                                 aaaCTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGc                  >  2:612507/1‑149 (MQ=255)                                                                                                                                 aaaCTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGc                  >  2:956656/1‑149 (MQ=255)                                                                                                                                  aaCTGGTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGt               >  2:980923/1‑151 (MQ=255)                                                                                                                                      ggTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTg             <  2:744690/149‑1 (MQ=255)                                                                                                                                      ggTTCGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCa           <  1:760639/151‑1 (MQ=255)                                                                                                                                          cGTACTCTGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTa       <  1:252146/151‑1 (MQ=255)                                                                                                                                               tctGGCCGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCt  >  1:163373/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 AGGTCAAAGCCGCTGCCTTTGAAGGTGAGCTGATCCCGGCGTCTCAGATCGACCGCCTGGCAACTCTGCCGACCTACGAAGAAGCAATTGCACGCCTGATGGCAACCATGAAAGAAGCTTCGGCTGGCAAACTGGTTCGTACTCTGGCTGCTGTACGCGATGCGAAAGAAGCTGCTTAATCGCAGTTATCTTTTTAACGCATTCGCTTACGTATAAACTTATTCTGATATTCAGGAACAATTTAAATGTCTATCACTAAAGATCAAATCATTGAAGCAGTTGCAGCTATGTCT  >  NC_000913/4180315‑4180607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑