Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,407,982	T→C	R443R (CGT→CGC)	rnr →	exoribonuclease R, RNase R

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,407,982	0	T	C	100.0%	321.2 / NA	83	R443R (CGT→CGC)	rnr	exoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/34);  total (49/34)

GGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAA  >  NC_000913/4407835‑4408129                                                                                                                                                    |                                                                                                                                                    ggATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCAtt                                                                                                                                                  <  2:270082/151‑1 (MQ=255)   aTCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTg                                                                                                                                                 >  2:890224/1‑150 (MQ=255)   aTCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTg                                                                                                                                                 >  2:464976/1‑150 (MQ=255)       gcgcGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGt                                                                                                                                            <  1:936720/151‑1 (MQ=255)       gcgcGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGt                                                                                                                                            <  1:26786/151‑1 (MQ=255)        cgcgAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTa                                                                                                                                           <  2:958915/151‑1 (MQ=255)         gcgAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTAt                                                                                                                                          >  2:354600/1‑151 (MQ=255)            aGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCg                                                                                                                                        >  1:171279/1‑150 (MQ=255)             gCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGaa                                                                                                                                      <  2:134147/151‑1 (MQ=255)                     ccccGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCa                                                                                                                              <  2:271521/151‑1 (MQ=255)                      cccGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGGGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGAcc                                                                                                                                >  2:850882/1‑148 (MQ=255)                       ccGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGc                                                                                                                            >  1:713688/1‑151 (MQ=255)                       ccGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGc                                                                                                                            <  1:30599/151‑1 (MQ=255)                        cGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCg                                                                                                                           <  1:175404/151‑1 (MQ=255)                            ggTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTaa                                                                                                                        <  1:302709/150‑1 (MQ=255)                            ggTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAac                                                                                                                       >  2:684500/1‑151 (MQ=255)                              ttAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacg                                                                                                                      >  1:193105/1‑150 (MQ=255)                                aaGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacgacg                                                                                                                   >  2:641398/1‑151 (MQ=255)                                aaGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacgac                                                                                                                    <  2:495681/150‑1 (MQ=255)                                 aGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGAcgc                                                                                                                  >  1:681684/1‑151 (MQ=255)                                    aTCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGAcgcg                                                                                                                 >  2:431600/1‑149 (MQ=255)                                    aTCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGAcgcg                                                                                                                 <  1:431649/149‑1 (MQ=255)                                      ctcGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGcac                                                                                                              >  1:621304/1‑150 (MQ=255)                                         gAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacga                                                                                                                     <  2:225028/140‑1 (MQ=255)                                         gAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAacga                                                                                                                     >  1:225056/1‑140 (MQ=255)                                           agagTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAaatt                                                                                                         >  2:527090/1‑150 (MQ=255)                                            gagTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAaatta                                                                                                        >  2:973823/1‑150 (MQ=255)                                             agTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAaattaat                                                                                                      >  1:178206/1‑151 (MQ=255)                                                 gCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGa                                                                                                   >  1:913449/1‑150 (MQ=255)                                                   aTAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGa                                                                                                   >  2:557999/1‑148 (MQ=255)                                                   aTAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGa                                                                                                   >  2:249153/1‑148 (MQ=255)                                                   aTAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGa                                                                                                   >  1:98208/1‑148 (MQ=255)                                                   aTAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAaga                                                                                                <  2:929293/151‑1 (MQ=255)                                                        ctctATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGc                                                                                            >  1:658827/1‑150 (MQ=255)                                                            ataAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCAt                                                                                          <  2:141136/148‑1 (MQ=255)                                                            ataAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGa                                                                                        <  2:643081/150‑1 (MQ=255)                                                             taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGAtt                                                                                      >  2:858627/1‑151 (MQ=255)                                                                aGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTg                                                                                   >  2:269739/1‑151 (MQ=255)                                                                 gTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTgg                                                                                  <  1:545877/151‑1 (MQ=255)                                                                 gTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTgg                                                                                  <  2:966932/151‑1 (MQ=255)                                                                      ggATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAat                                                                             >  2:566103/1‑151 (MQ=255)                                                                      ggATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAat                                                                             >  2:211489/1‑151 (MQ=255)                                                                       gATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAat                                                                             >  1:598628/1‑150 (MQ=255)                                                                        aTAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAatat                                                                           >  1:802428/1‑151 (MQ=255)                                                                          aaaGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATAtct                                                                         >  2:678872/1‑151 (MQ=255)                                                                           aaGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATAtctc                                                                        >  2:388553/1‑151 (MQ=255)                                                                           aaGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATAtctc                                                                        >  1:875913/1‑151 (MQ=255)                                                                              cccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTcggc                                                                     <  2:445249/151‑1 (MQ=255)                                                                                   gaagaaCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGgcgc                                                                >  2:277285/1‑151 (MQ=255)                                                                                    aagaaCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGcgcg                                                               >  1:337904/1‑151 (MQ=255)                                                                                        aCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTc                                                           >  2:664685/1‑151 (MQ=255)                                                                                        aCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTc                                                           <  2:507049/151‑1 (MQ=255)                                                                                            ggTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTg                                                       <  2:55181/151‑1 (MQ=255)                                                                                               gggATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTgag                                                     <  2:740875/150‑1 (MQ=255)                                                                                                ggATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTgaga                                                    >  1:840205/1‑150 (MQ=255)                                                                                                ggATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTgaga                                                    >  2:787002/1‑150 (MQ=255)                                                                                                     tcATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCg                                               >  1:916717/1‑150 (MQ=255)                                                                                                      cATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGaaa                                                  <  2:929864/146‑1 (MQ=255)                                                                                                       aTTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGaaa                                            <  2:913370/151‑1 (MQ=255)                                                                                                            agagCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAAcc                                       <  1:999885/151‑1 (MQ=255)                                                                                                               gCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGc                                    <  2:164020/151‑1 (MQ=255)                                                                                                                  aagaagCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACt                                 <  1:300957/151‑1 (MQ=255)                                                                                                                    gaagCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGt                               >  1:595029/1‑151 (MQ=255)                                                                                                                    gaagCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGt                               >  1:301147/1‑151 (MQ=255)                                                                                                                    gaagCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGt                               <  1:926938/151‑1 (MQ=255)                                                                                                                     aagCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGtt                              >  1:533726/1‑151 (MQ=255)                                                                                                                      agCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTc                             <  1:249180/151‑1 (MQ=255)                                                                                                                       gCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTcc                            <  2:460806/151‑1 (MQ=255)                                                                                                                       gCGAAGTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTcc                            >  1:865568/1‑151 (MQ=255)                                                                                                                            gTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTAtt                       >  2:348057/1‑151 (MQ=255)                                                                                                                            gTTCATTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTAtt                       <  2:885027/151‑1 (MQ=255)                                                                                                                                aTTTTCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACg                   >  2:757115/1‑151 (MQ=255)                                                                                                                                 ttttCAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACg                   >  2:127964/1‑150 (MQ=255)                                                                                                                                     cAACGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACaa               <  1:496992/150‑1 (MQ=255)                                                                                                                                      aaCGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAg              <  2:132411/150‑1 (MQ=255)                                                                                                                                      aaCGCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGc             >  2:313737/1‑151 (MQ=255)                                                                                                                                         gCTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCg           >  1:444730/1‑150 (MQ=255)                                                                                                                                          cTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCg           >  1:18382/1‑149 (MQ=255)                                                                                                                                          cTGAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCg           >  2:376253/1‑149 (MQ=255)                                                                                                                                            gAACGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAg         <  1:647848/149‑1 (MQ=255)                                                                                                                                             aaCGCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCAc      >  2:666613/1‑151 (MQ=255)                                                                                                                                               cgccgcATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCg    >  2:379850/1‑151 (MQ=255)                                                                                                                                                gCCGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCg    >  2:952095/1‑150 (MQ=255)                                                                                                                                                 ccGCATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGaa  <  1:503293/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAACGACGCGCACAAATTAATTGAAGAGTGCATGATTCTGGCGAATATCTCGGCGGCGCGTTTCGTTGAGAAAGCGAAAGAACCGGCACTGTTCCGTATTCACGACAAGCCGAGCACCGAA  >  NC_000913/4407835‑4408129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑