Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	376,673	T→C	intergenic (+3/‑99)	mhpT → / → yaiL	3‑hydroxyphenylpropionic transporter/DUF2058 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	376,673	0	T	C	100.0%	198.1 / NA	55	intergenic (+3/‑99)	mhpT/yaiL	3‑hydroxyphenylpropionic transporter/DUF2058 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/24);  total (31/24)

TACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGATTAGT  >  NC_000913/376528‑376818                                                                                                                                                  |                                                                                                                                                  tACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCtt                                                                                                                                              >  1:406669/1‑151 (MQ=255)   cTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTCTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCtta                                                                                                                                             <  2:905100/150‑1 (MQ=255)    tGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCttatt                                                                                                                                           >  1:248621/1‑151 (MQ=255)     ggCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCttatt                                                                                                                                           <  2:721949/150‑1 (MQ=255)       ccGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAgg                                                                                                                                        >  1:387324/1‑151 (MQ=255)            aaaaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAggg                                                                                                                                   <  1:939690/151‑1 (MQ=255)              aaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAgggg                                                                                                                                  >  2:467971/1‑150 (MQ=255)              aaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGc                                                                                                                                 <  2:7238/151‑1 (MQ=255)              aaTGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGATTTTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGACGTGCGCCGATGCCTGCCGCGCCTTATTAg                                                                                                                                         <  2:722117/143‑1 (MQ=255)                  cTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGaa                                                                                                                              >  1:28538/1‑150 (MQ=255)                   tGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGaa                                                                                                                              >  1:832483/1‑149 (MQ=255)                   tGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGaa                                                                                                                              >  1:793791/1‑149 (MQ=255)                    ggCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAgg                                                                                                                           <  1:30271/151‑1 (MQ=255)                       aTTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGAc                                                                                                                        >  1:427575/1‑151 (MQ=255)                        ttAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACt                                                                                                                       >  1:891991/1‑151 (MQ=255)                          aGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTc                                                                                                                     <  2:69412/151‑1 (MQ=255)                             cACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCAcc                                                                                                                  >  1:90050/1‑151 (MQ=255)                              aCTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCAccc                                                                                                                 >  2:822275/1‑151 (MQ=255)                                    aCGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATac                                                                                                           <  2:224757/151‑1 (MQ=255)                                       gTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACAcc                                                                                                        >  1:428709/1‑151 (MQ=255)                                        tCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCt                                                                                                       >  2:70592/1‑151 (MQ=255)                                                  ggCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTa                                                                                             <  2:138659/151‑1 (MQ=255)                                                     cGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTa                                                                                             >  1:248525/1‑148 (MQ=255)                                                         tCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGt                                                                                        <  1:292900/149‑1 (MQ=255)                                                              aCCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTc                                                                                  >  2:383706/1‑150 (MQ=255)                                                                  ggTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaa                                                                             <  2:228884/151‑1 (MQ=255)                                                                     aTTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTaaaaa                                                                          <  1:135933/151‑1 (MQ=255)                                                                       tCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAat                                                                         <  2:763851/150‑1 (MQ=255)                                                                        cTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAatt                                                                        >  1:6876/1‑150 (MQ=255)                                                                            ttgCGGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCCCGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCAACCCCATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATAATTc                                                                    <  2:570027/150‑1 (MQ=255)                                                                              gCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAg                                                                  <  1:144049/150‑1 (MQ=255)                                                                              gCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGc                                                                 <  1:390714/151‑1 (MQ=255)                                                                                 gggTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCggg                                                              >  2:991463/1‑151 (MQ=255)                                                                                         ggTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGaa                                                       >  1:602628/1‑150 (MQ=255)                                                                                            gtTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACa                                                    <  2:217377/150‑1 (MQ=255)                                                                                                 ttttGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATg                                              >  1:818039/1‑151 (MQ=255)                                                                                                 ttttGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATg                                              >  1:287790/1‑151 (MQ=255)                                                                                                  tttGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATgg                                             >  1:957497/1‑151 (MQ=255)                                                                                                      atgaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaaa                                         <  1:81224/151‑1 (MQ=255)                                                                                                        gaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCaaaa                                        <  1:5469/150‑1 (MQ=255)                                                                                                        gaGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAAc                                       >  2:365204/1‑151 (MQ=255)                                                                                                         aGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAAct                                      <  1:436664/151‑1 (MQ=255)                                                                                                          gCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAActt                                     >  1:616828/1‑151 (MQ=255)                                                                                                               agaTCACGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAActtacctt                                >  2:148684/1‑151 (MQ=255)                                                                                                                    aCGAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACa                             >  1:408633/1‑149 (MQ=255)                                                                                                                      gAATACAGCCGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAaga                          <  2:627075/150‑1 (MQ=255)                                                                                                                              ccGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCt                 <  2:531028/151‑1 (MQ=255)                                                                                                                              ccGTGCGCCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCt                 <  2:377587/151‑1 (MQ=255)                                                                                                                                   cgcCGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCaa              >  1:556705/1‑149 (MQ=255)                                                                                                                                     ccGATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCa          <  1:441246/151‑1 (MQ=255)                                                                                                                                       gATGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAgg        >  1:874614/1‑151 (MQ=255)                                                                                                                                        aTGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAgg        >  1:411895/1‑150 (MQ=255)                                                                                                                                         tGCCTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGAt      >  2:680719/1‑151 (MQ=255)                                                                                                                                            cTGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGAtt     >  1:398353/1‑149 (MQ=255)                                                                                                                                             tGACGCGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGATTAGt  >  2:991539/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                  TACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAGCCGTGCGCCGATGCCTGACGTGCCTTATTAGGCAGGGGCGAAAGGGACTTCACCCCAATACACCTACGGCGTTTACAGGTATACTCGCTAAAAATTATTCAGCGGGTTTGGAAACAAAGATGGCAAAACTTACCTTACAAGAGCAGTTGCTCAAAGCAGGATTAGT  >  NC_000913/376528‑376818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑