Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	487,092	T→C	L186P (CTG→CCG)	mscK →	mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	487,092	0	T	C	100.0%	313.5 / NA	86	L186P (CTG→CCG)	mscK	mechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/42);  total (44/42)

GGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCTGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCA  >  NC_000913/486952‑487239                                                                                                                                             |                                                                                                                                                    ggCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACt                                                                                                                                           <  1:865260/151‑1 (MQ=255)    gcTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGAt                                                                                                                                        <  2:382443/151‑1 (MQ=255)       ggACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTc                                                                                                                                     <  2:198976/151‑1 (MQ=255)       ggACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTc                                                                                                                                     <  2:461530/151‑1 (MQ=255)       ggACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTc                                                                                                                                     <  2:488196/151‑1 (MQ=255)        gACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCg                                                                                                                                    >  2:381800/1‑151 (MQ=255)        gACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCg                                                                                                                                    <  2:546915/151‑1 (MQ=255)         aCGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCgg                                                                                                                                   <  1:106984/151‑1 (MQ=255)          cGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGc                                                                                                                                  <  1:323391/151‑1 (MQ=255)           gATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                 >  1:271898/1‑151 (MQ=255)            aTTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCga                                                                                                                                <  2:374709/151‑1 (MQ=255)             tttGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                 >  2:823502/1‑149 (MQ=255)             tttGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                 >  2:561124/1‑149 (MQ=255)              ttGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCg                                                                                                                                 >  2:790165/1‑148 (MQ=255)                      cGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTa                                                                                                                      <  1:409436/151‑1 (MQ=255)                      cGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTa                                                                                                                      <  2:919996/151‑1 (MQ=255)                       gCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTAc                                                                                                                     <  1:357883/151‑1 (MQ=255)                        cacaAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACg                                                                                                                    >  2:373903/1‑151 (MQ=255)                        cacaAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACg                                                                                                                    >  1:620346/1‑151 (MQ=255)                         acaAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGt                                                                                                                   >  1:653600/1‑151 (MQ=255)                          caAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTc                                                                                                                  >  2:63889/1‑151 (MQ=255)                           aaaaCGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTcc                                                                                                                 <  1:597106/151‑1 (MQ=255)                            aaaCGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTccc                                                                                                                >  2:575387/1‑151 (MQ=255)                               cGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagc                                                                                                             >  1:785982/1‑151 (MQ=255)                                  tCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagcc                                                                                                            >  1:367001/1‑149 (MQ=255)                                     ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCccagc                                                                                                             <  2:261038/145‑1 (MQ=255)                                     ggCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGaa                                                                                                        >  2:544156/1‑150 (MQ=255)                                          cTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGtt                                                                                                  >  1:221948/1‑151 (MQ=255)                                           ttATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGtt                                                                                                  >  1:350226/1‑150 (MQ=255)                                            tataACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAgtgt                                                                                                   <  2:454603/148‑1 (MQ=255)                                            tataACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGtt                                                                                                  <  1:136619/149‑1 (MQ=255)                                            tataACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTa                                                                                                 <  2:490959/150‑1 (MQ=255)                                            tataACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTa                                                                                                 >  1:845628/1‑150 (MQ=255)                                             ataACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTaa                                                                                                <  1:299224/150‑1 (MQ=255)                                             ataACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAAt                                                                                               <  2:747860/151‑1 (MQ=255)                                              taACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATg                                                                                              >  2:358126/1‑151 (MQ=255)                                                 cagccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGc                                                                                             >  1:538776/1‑149 (MQ=255)                                                 cagccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAg                                                                                           <  2:221920/151‑1 (MQ=255)                                                   gccagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGc                                                                                         <  1:863386/151‑1 (MQ=255)                                                     cagcTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGccc                                                                                       >  2:685673/1‑151 (MQ=255)                                                        cTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagc                                                                                    >  2:746964/1‑151 (MQ=255)                                                          ggTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagcag                                                                                  <  1:610497/151‑1 (MQ=255)                                                          ggTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagc                                                                                    >  2:925370/1‑149 (MQ=255)                                                          ggTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCcagc                                                                                    >  1:595862/1‑149 (MQ=255)                                                            tttCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAgg                                                                                 >  2:939796/1‑150 (MQ=255)                                                             ttCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCg                                                                               >  1:379298/1‑151 (MQ=255)                                                               cGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGtt                                                                             <  1:84117/151‑1 (MQ=255)                                                                gTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTg                                                                            <  1:614473/151‑1 (MQ=255)                                                                  tACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCt                                                                          >  1:86743/1‑151 (MQ=255)                                                                   aCAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTg                                                                         >  1:386222/1‑151 (MQ=255)                                                                      gACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAAt                                                                      <  1:248203/151‑1 (MQ=255)                                                                      gACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCACAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGaa                                                                       <  2:156793/150‑1 (MQ=255)                                                                        cGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGc                                                                    <  1:376383/151‑1 (MQ=255)                                                                             cccGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCg                                                                   >  2:747780/1‑147 (MQ=255)                                                                              ccGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGAt                                                              <  2:659487/151‑1 (MQ=255)                                                                               cGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGAtt                                                             >  2:526335/1‑151 (MQ=255)                                                                                gAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTg                                                            <  1:548484/151‑1 (MQ=255)                                                                                 aaCGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGa                                                           <  2:631337/151‑1 (MQ=255)                                                                                  aCGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGAc                                                          >  1:604214/1‑151 (MQ=255)                                                                                    gcgTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGAcc                                                         >  2:457882/1‑150 (MQ=255)                                                                                    gcgTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACca                                                        <  2:794800/151‑1 (MQ=255)                                                                                     cgTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGAcc                                                         >  2:299910/1‑149 (MQ=255)                                                                                      gTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACcagc                                                      <  2:823739/151‑1 (MQ=255)                                                                                         cAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCagcagc                                                   <  1:736747/151‑1 (MQ=255)                                                                                          aaaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCg                                                  <  1:341394/151‑1 (MQ=255)                                                                                          aaaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCg                                                  >  1:556304/1‑151 (MQ=255)                                                                                           aaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCg                                                  >  2:198793/1‑150 (MQ=255)                                                                                           aaaTGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGt                                                 <  2:884221/151‑1 (MQ=255)                                                                                              tGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTaa                                               >  2:753196/1‑150 (MQ=255)                                                                                                cGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGGATGCGGAGATTGACCAGCAGCGTaca                                              >  2:355169/1‑147 (MQ=255)                                                                                                  aTGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGcc                                          <  1:349595/151‑1 (MQ=255)                                                                                                   tGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCt                                         >  2:351147/1‑151 (MQ=255)                                                                                                    gTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCt                                         <  1:859126/150‑1 (MQ=255)                                                                                                        aaCGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGa                                      >  1:461184/1‑149 (MQ=255)                                                                                                          cGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAggg                                  >  1:461311/1‑151 (MQ=255)                                                                                                               cGCAGCAGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAacac                             <  1:358169/151‑1 (MQ=255)                                                                                                                gcagcaGCTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACAcc                            >  1:52382/1‑151 (MQ=255)                                                                                                                  agcagcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGt                          >  1:405156/1‑151 (MQ=255)                                                                                                                     agcTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCtt                       <  1:104377/151‑1 (MQ=255)                                                                                                                       cTGCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGc                     >  1:808887/1‑151 (MQ=255)                                                                                                                         gCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGc                     >  2:702582/1‑149 (MQ=255)                                                                                                                         gCAACAAATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGc                     <  2:451646/149‑1 (MQ=255)                                                                                                                          caacaaATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAgg                  >  1:758429/1‑151 (MQ=255)                                                                                                                             caaATTCGCAGTCGTCCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGAt                >  1:670882/1‑150 (MQ=255)                                                                                                                                         cgtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGc   <  2:379254/151‑1 (MQ=255)                                                                                                                                          gtcCGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCa  <  2:52379/151‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                    GGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGGCGTCTTATAACAGCCAGCTGGTTTCGTTACAGACGCAGCCCGAACGCGTGCAAAATGCGATGTATAACGCTTCGCAGCAGCTGCAACAAATTCGCAGTCGTCTGGATGGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCA  >  NC_000913/486952‑487239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑