Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 657,377 T→C V29A (GTG→GCG)  cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,3770TC100.0% 214.0 / NA 58V29A (GTG→GCG) cspEconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/24);  total (34/24)

AATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAT  >  NC_000913/657231‑657518
                                                                                                                                                  |                                                                                                                                             
aaTTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTc                                                                                                                                           >  2:634825/1‑151 (MQ=255)
   tGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGt                                                                                                                                         >  2:437831/1‑150 (MQ=255)
         aCTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTtc                                                                                                                                  >  2:576348/1‑151 (MQ=255)
             gTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTg                                                                                                                              <  1:538035/151‑1 (MQ=255)
                    acacAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATcc                                                                                                                        >  1:32822/1‑150 (MQ=255)
                      acaGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGa                                                                                                                     <  1:235795/151‑1 (MQ=255)
                        aGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACt                                                                                                                   >  2:440950/1‑151 (MQ=255)
                         gCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTa                                                                                                                  <  1:357942/151‑1 (MQ=255)
                             ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATgg                                                                                                              >  2:224783/1‑151 (MQ=255)
                             ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATgg                                                                                                              >  1:764190/1‑151 (MQ=255)
                             ttGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATgg                                                                                                              >  1:630797/1‑151 (MQ=255)
                              tgtgTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGt                                                                                                             >  2:931685/1‑151 (MQ=255)
                                 gtCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGtttt                                                                                                          >  2:826676/1‑151 (MQ=255)
                                      tttttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAAc                                                                                                     >  2:69638/1‑151 (MQ=255)
                                         ttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAActct                                                                                                  >  2:239688/1‑151 (MQ=255)
                                         ttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAActct                                                                                                  >  1:260050/1‑151 (MQ=255)
                                         ttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAActc                                                                                                   <  1:405899/150‑1 (MQ=255)
                                         ttCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAActc                                                                                                   <  1:808034/150‑1 (MQ=255)
                                          tCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCtt                                                                                                 >  1:24852/1‑151 (MQ=255)
                                                aaaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGa                                                                                            >  1:753641/1‑150 (MQ=255)
                                                aaaGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGa                                                                                            <  1:768745/150‑1 (MQ=255)
                                                  aGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAgg                                                                                         >  2:672837/1‑151 (MQ=255)
                                                  aGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAgg                                                                                         >  2:712700/1‑151 (MQ=255)
                                                      aaTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCa                                                                                      <  1:291794/150‑1 (MQ=255)
                                                         tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAgcg                                                                                   <  2:507988/150‑1 (MQ=255)
                                                         tttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGGTTTAAAACTCTTGCTGAAGGTCAgcg                                                                                   >  1:228787/1‑150 (MQ=255)
                                                          ttGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGcgcg                                                                                 >  2:842345/1‑151 (MQ=255)
                                                             aTGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTag                                                                              <  1:347200/151‑1 (MQ=255)
                                                             aTGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTag                                                                              <  1:444195/151‑1 (MQ=255)
                                                                   aaGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCg                                                                        >  1:126216/1‑151 (MQ=255)
                                                                    aGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGa                                                                       <  1:576411/151‑1 (MQ=255)
                                                                         aaaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATc                                                                   <  2:440036/150‑1 (MQ=255)
                                                                              tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAc                                                             >  1:400121/1‑151 (MQ=255)
                                                                                aCGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACgg                                                           >  1:970773/1‑151 (MQ=255)
                                                                                 cGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGt                                                          >  2:167525/1‑151 (MQ=255)
                                                                                  gTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTg                                                         >  1:627102/1‑151 (MQ=255)
                                                                                     aaGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGcc                                                       >  2:979813/1‑150 (MQ=255)
                                                                                                  aGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgc                                         <  1:98290/151‑1 (MQ=255)
                                                                                                     ccAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTctgctg                                       <  2:750232/150‑1 (MQ=255)
                                                                                                      cAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCa                                     <  1:182040/151‑1 (MQ=255)
                                                                                                       aaaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCa                                     >  2:430708/1‑150 (MQ=255)
                                                                                                        aaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCaa                                    >  1:520356/1‑150 (MQ=255)
                                                                                                         aGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAAc                                  <  1:576267/151‑1 (MQ=255)
                                                                                                             ttCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTa                               >  1:151774/1‑150 (MQ=255)
                                                                                                              tCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAAt                             >  2:354957/1‑151 (MQ=255)
                                                                                                                ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                           >  1:694993/1‑151 (MQ=255)
                                                                                                                ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                           >  2:310319/1‑151 (MQ=255)
                                                                                                                ggTTTCATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCg                           <  2:414930/151‑1 (MQ=255)
                                                                                                                     cATTACTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTg                      <  2:776835/151‑1 (MQ=255)
                                                                                                                         aCTCCGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTaa                   <  1:669232/150‑1 (MQ=255)
                                                                                                                             cGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAc              <  1:793409/151‑1 (MQ=255)
                                                                                                                             cGGAAGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATAc              >  1:678568/1‑151 (MQ=255)
                                                                                                                                 aGACGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCa          <  1:373464/151‑1 (MQ=255)
                                                                                                                                    cGGCAGCAAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCa       <  2:361649/151‑1 (MQ=255)
                                                                                                                                      gcagcaAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAg     <  2:328899/151‑1 (MQ=255)
                                                                                                                                       cagcaAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCa       >  2:40748/1‑148 (MQ=255)
                                                                                                                                         gcaAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAt  >  1:538271/1‑151 (MQ=255)
                                                                                                                                         gcaAAGACGCGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGATGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAt  <  1:826752/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
AATTGGTGTACTGGTAACCGACACAGCATTTGTGTCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAAT  >  NC_000913/657231‑657518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: