Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 702,598 A→G S52P (TCC→CCC)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913702,5980AG97.6% 149.0 / ‑2.5 42coding (154/1149 nt)nagAN‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (22/19);  minor base . (0/1);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

GCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAAT  >  NC_000913/702457‑702743
                                                                                                                                             |                                                                                                                                                 
gCGTCGGCAGATCGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGGGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGc                                                                                                                                          >  1:405293/1‑151 (MQ=255)
 agTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGcc                                                                                                                                         >  2:252078/2‑151 (MQ=255)
  gTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGccc                                                                                                                                        >  1:149180/1‑151 (MQ=255)
        aGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTc                                                                                                                                   >  2:941835/1‑150 (MQ=255)
         gATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCa                                                                                                                                  <  2:544458/150‑1 (MQ=255)
               tagtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAAc                                                                                                                            <  2:962402/150‑1 (MQ=255)
                agtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACgt                                                                                                                          <  1:248928/151‑1 (MQ=255)
                agtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACg                                                                                                                           <  2:448323/150‑1 (MQ=255)
                   aCAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACgttgt                                                                                                                       <  2:900216/151‑1 (MQ=255)
                   aCAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACgttgt                                                                                                                       >  2:150087/1‑151 (MQ=255)
                   aCAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACgttg                                                                                                                        <  1:511134/150‑1 (MQ=255)
                       ccTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGG‑GAGAATGGCCCCGTTCAGTGAACGTTGTTCGAt                                                                                                                  <  2:976994/151‑1 (MQ=255)
                                  aTTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTggcgg                                                                                                         >  2:270148/1‑150 (MQ=255)
                                    tGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCa                                                                                                       <  2:939096/150‑1 (MQ=255)
                                      gCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGtt                                                                                                    >  2:857729/1‑151 (MQ=255)
                                         ttCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTcc                                                                                                  <  2:405245/150‑1 (MQ=255)
                                         ttCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTcc                                                                                                  <  1:472060/150‑1 (MQ=255)
                                           cTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGAGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCt                                                                                               >  1:991061/1‑151 (MQ=255)
                                                 gATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGa                                                                                         <  1:538329/151‑1 (MQ=255)
                                                     tCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAg                                                                                      <  1:515853/150‑1 (MQ=255)
                                                      ccAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGAc                                                                                    >  2:111737/1‑151 (MQ=255)
                                                        aGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTACACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGc                                                                                  >  1:207150/1‑151 (MQ=255)
                                                          cGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCtt                                                                                >  2:267535/1‑151 (MQ=255)
                                                                cACGCTGACCGCTTCAGCGGTGGCGTTAAACTGTACGCCGCCGCAGACGTTTAACTGAACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGGTCCGCTACCGGACAGACGCTTTTaa                                                                            >  1:765877/1‑149 (MQ=255)
                                                                   gCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAgg                                                                       >  1:329017/1‑151 (MQ=255)
                                                                     tGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGcc                                                                     >  1:125072/1‑151 (MQ=255)
                                                                              cAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGAt                                                            >  2:517772/1‑151 (MQ=255)
                                                                                    tgtCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATaacaac                                                      >  1:135637/1‑151 (MQ=255)
                                                                                    tgtCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATaacaac                                                      >  2:319241/1‑151 (MQ=255)
                                                                                        gTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACcgcg                                                  >  2:18267/1‑151 (MQ=255)
                                                                                          tAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGt                                                 <  1:994704/150‑1 (MQ=255)
                                                                                                gTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGtca                                            <  2:205803/149‑1 (MQ=255)
                                                                                                    gccgctgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGa                                      <  1:731475/151‑1 (MQ=255)
                                                                                                      cgccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGaa                                     >  1:127085/1‑150 (MQ=255)
                                                                                                        ccgcAGCCGTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAAtt                                  <  2:454126/151‑1 (MQ=255)
                                                                                                                gTTTAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCg                          >  2:448640/1‑151 (MQ=255)
                                                                                                                 tttAACTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCgg                         >  1:328140/1‑151 (MQ=255)
                                                                                                                    aaCTGCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTaa                      <  1:319280/151‑1 (MQ=255)
                                                                                                                        gCACATCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGAt                  <  1:482172/151‑1 (MQ=255)
                                                                                                                           caTCGATAAAACCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCg               <  2:53143/151‑1 (MQ=255)
                                                                                                                                  aaaaCCGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTgg        <  1:197799/151‑1 (MQ=255)
                                                                                                                                      ccGGGGGGGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGtt     >  1:251810/1‑150 (MQ=255)
                                                                                                                                        ggggggGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAAt  >  1:963917/1‑151 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAGCGATAACAACCGCGTGGTCATCAAGAAATTCGTGGCCGGTAAAGATCCGGCCCTGGGTTAAT  >  NC_000913/702457‑702743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑