Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	736,382	T→C	intergenic (+163/‑63)	ybfC → / → ybfQ	putative secreted protein/pseudogene, H repeat‑associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	736,382	0	T	C	100.0%	226.2 / NA	59	intergenic (+163/‑63)	ybfC/ybfQ	putative secreted protein/pseudogene, H repeat‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

AAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGTCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGAT  >  NC_000913/736234‑736528                                                                                                                                                     |                                                                                                                                                   aaaaCAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCt                                                                                                                                                  >  1:336090/1‑151 (MQ=255)  aaaCAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTa                                                                                                                                                 <  1:216860/151‑1 (MQ=255)    aCAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTaaa                                                                                                                                               <  2:737857/151‑1 (MQ=255)     cAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAg                                                                                                                                              <  2:679898/151‑1 (MQ=255)      aaaTTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAg                                                                                                                                              >  2:207637/1‑150 (MQ=255)             tCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGttttt                                                                                                                                      >  1:824619/1‑151 (MQ=255)                 tgtgAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCAt                                                                                                                                   <  2:17429/150‑1 (MQ=255)                          ttATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATAc                                                                                                                          >  1:477994/1‑150 (MQ=255)                           tatataACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACAt                                                                                                                        >  2:78916/1‑151 (MQ=255)                            atataACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACAt                                                                                                                        <  1:937440/150‑1 (MQ=255)                            atataACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATg                                                                                                                       <  1:329252/151‑1 (MQ=255)                               taACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATc                                                                                                                    >  2:401782/1‑151 (MQ=255)                                   tCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAAt                                                                                                                >  1:914777/1‑151 (MQ=255)                                   tCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAAt                                                                                                                >  2:357286/1‑151 (MQ=255)                                      tGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACt                                                                                                             >  2:94101/1‑151 (MQ=255)                                        gAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTcc                                                                                                           >  2:45482/1‑151 (MQ=255)                                        gAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTcc                                                                                                           >  1:31721/1‑151 (MQ=255)                                             ggTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACa                                                                                                      <  1:122614/151‑1 (MQ=255)                                                   tATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAGGTTCAACAAGTGAGATACTGCTTATTACCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACataaat                                                                                                 >  2:280084/1‑150 (MQ=255)                                                    aTTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACataaata                                                                                                <  2:455122/150‑1 (MQ=255)                                                       ttGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGt                                                                                            >  1:500759/1‑151 (MQ=255)                                                         gCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTg                                                                                           >  2:557049/1‑150 (MQ=255)                                                           gTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAAc                                                                                        >  1:506193/1‑151 (MQ=255)                                                                  ttGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATgg                                                                                  >  1:57299/1‑150 (MQ=255)                                                                   tGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGa                                                                                 >  2:610546/1‑150 (MQ=255)                                                                    gCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAAc                                                                               <  1:762784/151‑1 (MQ=255)                                                                      aGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACtt                                                                             <  2:712788/151‑1 (MQ=255)                                                                      aGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACtt                                                                             >  2:961578/1‑151 (MQ=255)                                                                        ggAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaa                                                                           <  1:997088/151‑1 (MQ=255)                                                                        ggAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaa                                                                           <  2:350704/151‑1 (MQ=255)                                                                           aaGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaaaaa                                                                        <  2:381141/151‑1 (MQ=255)                                                                            aGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaaaaaa                                                                       <  1:489201/151‑1 (MQ=255)                                                                            aGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTaaaaaa                                                                       <  2:298770/151‑1 (MQ=255)                                                                                 aCGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACAAGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGAt                                                                  <  1:628331/151‑1 (MQ=255)                                                                                     aaCGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGa                                                               <  2:824542/150‑1 (MQ=255)                                                                                      aCGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAAc                                                             <  1:701201/151‑1 (MQ=255)                                                                                           aCTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATAtt                                                        >  2:178851/1‑151 (MQ=255)                                                                                              tttttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTc                                                      <  2:88399/150‑1 (MQ=255)                                                                                              tttttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTc                                                      <  2:583118/150‑1 (MQ=255)                                                                                               ttttGGACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACACATGGAACTTAAAAAATTGATGGAACATATTTCt                                                     >  2:468892/1‑150 (MQ=255)                                                                                                 ttGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAtt                                                  >  1:113955/1‑151 (MQ=255)                                                                                                  tGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTAtt                                                  <  2:98828/150‑1 (MQ=255)                                                                                                        aaaTAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACAcc                                             <  1:205537/149‑1 (MQ=255)                                                                                                            aatTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGAtt                                        <  2:267663/150‑1 (MQ=255)                                                                                                            aatTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGAtt                                        >  1:589822/1‑150 (MQ=255)                                                                                                             atTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTAc                                      >  1:926672/1‑151 (MQ=255)                                                                                                               tCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACa                                     <  1:644656/150‑1 (MQ=255)                                                                                                                cGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGa                                   <  2:477943/151‑1 (MQ=255)                                                                                                                cGAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGa                                   <  2:960126/151‑1 (MQ=255)                                                                                                                 gAATTTTAAGTTCAACAATTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGAc                                  >  1:317131/1‑151 (MQ=255)                                                                                                                             caacaaTTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAg                      <  2:847588/151‑1 (MQ=255)                                                                                                                              aacaaTTGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAg                      >  2:878049/1‑150 (MQ=255)                                                                                                                                    tGAGATACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGc                >  1:461090/1‑150 (MQ=255)                                                                                                                                     gagaTACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCAt              >  2:206485/1‑151 (MQ=255)                                                                                                                                      agaTACTACTTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCAt              >  1:204419/1‑150 (MQ=255)                                                                                                                                             actTATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTc      <  2:360735/151‑1 (MQ=255)                                                                                                                                                tATTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCa     <  1:641281/149‑1 (MQ=255)                                                                                                                                                 aTTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTc      >  2:84225/1‑147 (MQ=255)                                                                                                                                                 aTTGCCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGat  <  2:287133/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AAAACAAATTAATCATTGTGAAAAATTATATAACTCATGGAATAGGTGTTTATTTTGCGTTCCATTTGCAGGGAAAGATCACGTAACGCTACTTTTTTGTACTAAATAATTCGAATTTTAAGTTCAACAATTGAGATACTACTTATTGTCTAAAGCTGTTTTTCATAGCTTATACATGATCAAATACTCCTTACATAAATAAGGTGAACAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTACACCCGATTACAGACAAGCCTGGAAAGTGGTGCATAAATTGTCAGAT  >  NC_000913/736234‑736528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑