Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	851,958	G→A	P214S (CCG→TCG)	opgE ←	OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	851,958	0	G	A	100.0%	312.5 / NA	81	P214S (CCG→TCG)	opgE	OPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/45);  total (36/45)

GTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGGCTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTAAATACG  >  NC_000913/851810‑852103                                                                                                                                                     |                                                                                                                                                  gTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACt                                                                                                                                                 >  1:383672/1‑151 (MQ=255)  tGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACtt                                                                                                                                                >  1:784254/1‑151 (MQ=255)    gAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTaa                                                                                                                                              <  1:679896/151‑1 (MQ=255)    gAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTaa                                                                                                                                              <  1:428391/151‑1 (MQ=255)      gCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAAc                                                                                                                                             <  2:295944/150‑1 (MQ=255)      gCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACg                                                                                                                                            <  1:723708/151‑1 (MQ=255)        cACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGc                                                                                                                                          <  1:888537/151‑1 (MQ=255)           tGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGt                                                                                                                                       >  1:382002/1‑151 (MQ=255)            ggcggcAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATTCTTCGACTTAACGGCAGt                                                                                                                                       <  1:823065/150‑1 (MQ=255)            ggcggcAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAg                                                                                                                                        <  2:712562/149‑1 (MQ=255)            ggcggcAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGt                                                                                                                                       <  1:763694/150‑1 (MQ=255)              cggcAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGAc                                                                                                                                    >  2:135809/1‑151 (MQ=255)              cggcAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGAc                                                                                                                                    >  1:221388/1‑151 (MQ=255)              cggcAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGAc                                                                                                                                    >  2:139641/1‑151 (MQ=255)                  aaTGTAGTCAGCAAATATCAAACCGTAAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGt                                                                                                                                 <  2:943972/150‑1 (MQ=255)                    tGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTcc                                                                                                                               >  2:567979/1‑150 (MQ=255)                       aGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAgg                                                                                                                            <  1:156244/150‑1 (MQ=255)                       aGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGt                                                                                                                           >  1:827153/1‑151 (MQ=255)                       aGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGt                                                                                                                           >  2:43692/1‑151 (MQ=255)                          cAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTAt                                                                                                                         <  1:299691/150‑1 (MQ=255)                              aaaTATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTc                                                                                                                    <  1:322768/151‑1 (MQ=255)                              aaaTATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTc                                                                                                                    >  2:348932/1‑151 (MQ=255)                              aaaTATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTc                                                                                                                    <  1:81810/151‑1 (MQ=255)                               aaTATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCa                                                                                                                   <  1:511344/151‑1 (MQ=255)                               aaTATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCa                                                                                                                   <  2:936099/151‑1 (MQ=255)                                   tCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTcc                                                                                                               >  2:644394/1‑151 (MQ=255)                                     aaaCCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCgg                                                                                                             <  2:492365/151‑1 (MQ=255)                                     aaaCCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCgg                                                                                                             <  2:463630/151‑1 (MQ=255)                                        ccGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGaa                                                                                                           <  1:262091/150‑1 (MQ=255)                                            tAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGttt                                                                                                      >  2:236324/1‑151 (MQ=255)                                                gctgctGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGcc                                                                                                  <  2:755576/151‑1 (MQ=255)                                                   gctgGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCa                                                                                                 <  1:637489/149‑1 (MQ=255)                                                   gctgGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATc                                                                                               >  2:91074/1‑151 (MQ=255)                                                    ctgGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATcc                                                                                              >  1:377989/1‑151 (MQ=255)                                                      ggCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATcc                                                                                              <  1:903538/149‑1 (MQ=255)                                                      ggCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATcc                                                                                              <  1:336448/149‑1 (MQ=255)                                                       gCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGtt                                                                                           <  2:277374/151‑1 (MQ=255)                                                       gCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGtt                                                                                           <  2:63309/151‑1 (MQ=255)                                                          aaCGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCtt                                                                                        >  1:447389/1‑151 (MQ=255)                                                            cGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGc                                                                                      >  2:951300/1‑151 (MQ=255)                                                              gggTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGctc                                                                                    >  1:102059/1‑151 (MQ=255)                                                                  aTTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCCGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTc                                                                                 >  2:969100/1‑150 (MQ=255)                                                                    tGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAAt                                                                              >  1:29418/1‑151 (MQ=255)                                                                       cccAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCAc                                                                           >  2:10408/1‑151 (MQ=255)                                                                          aGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTc                                                                        >  1:874480/1‑151 (MQ=255)                                                                           gTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCg                                                                       <  1:845420/151‑1 (MQ=255)                                                                           gTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCg                                                                       <  1:979608/151‑1 (MQ=255)                                                                             gACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGtt                                                                     >  1:856177/1‑151 (MQ=255)                                                                             gACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGtt                                                                     >  1:170710/1‑151 (MQ=255)                                                                             gACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGt                                                                      <  2:32523/150‑1 (MQ=255)                                                                                cGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGGTTTTCCACCCGTTCTTCCTATTCAATCACCTCGttatt                                                                  <  2:677859/151‑1 (MQ=255)                                                                                   cAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTattatt                                                               >  2:831847/1‑151 (MQ=255)                                                                                        gCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGa                                                          <  1:477884/151‑1 (MQ=255)                                                                                         cACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACAGAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGAt                                                         <  2:314870/151‑1 (MQ=255)                                                                                            cGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGAc                                                      >  1:193088/1‑151 (MQ=255)                                                                                            cGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGAc                                                      <  1:491326/151‑1 (MQ=255)                                                                                               gggCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATc                                                   >  2:640611/1‑151 (MQ=255)                                                                                                  cATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCtt                                                <  1:236080/151‑1 (MQ=255)                                                                                                       cgACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCaa                                           <  1:797864/151‑1 (MQ=255)                                                                                                            cgcgcTTTCACCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACg                                       >  1:518796/1‑150 (MQ=255)                                                                                                                 tttCAACGATCAACACCACATAGGTCTGATACTTTGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTTCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACACCCTTAGCAAAACGaaa                                    <  2:687156/148‑1 (MQ=255)                                                                                                                  ttCTCCGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATa                                  <  1:334986/149‑1 (MQ=255)                                                                                                                       cGATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATa                           <  2:586967/151‑1 (MQ=255)                                                                                                                        gATCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGAt                            >  2:4309/1‑149 (MQ=255)                                                                                                                         aTCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGc                         >  1:501665/1‑151 (MQ=255)                                                                                                                         aTCACCACCACATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGc                         <  1:416420/151‑1 (MQ=255)                                                                                                                            accaccacATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGcc                        >  1:521529/1‑149 (MQ=255)                                                                                                                            accaccacATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGcc                        >  1:65823/1‑149 (MQ=255)                                                                                                                             ccaccacATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGt                     >  2:42270/1‑151 (MQ=255)                                                                                                                                ccacATAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATc                  >  1:552848/1‑151 (MQ=255)                                                                                                                                  acaTAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCtt                <  1:180371/151‑1 (MQ=255)                                                                                                                                    aTAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTc              >  2:31706/1‑151 (MQ=255)                                                                                                                                    aTAGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTc              <  2:352830/151‑1 (MQ=255)                                                                                                                                      aGGTCTGATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCa             <  1:521715/150‑1 (MQ=255)                                                                                                                                      aGGTCTGATACTTCGACTTAACGGCAGGGACCGTCCAGGTATCTTTCATTCCGGAAAGGTTTGCCATCCGGTCTTGCTCTTCAATCACCTCGTTATTATTGAAGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTc              >  2:840466/1‑149 (MQ=255)                                                                                                                                            gATACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTaaa      <  2:383627/151‑1 (MQ=255)                                                                                                                                              tACTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTaaa      >  1:618313/1‑149 (MQ=255)                                                                                                                                               aCTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTAAATa    <  1:135823/150‑1 (MQ=255)                                                                                                                                               aCTTCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTAAATAc   <  1:568038/151‑1 (MQ=255)                                                                                                                                                  tCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTAAATACg  <  2:784171/149‑1 (MQ=255)                                                                                                                                                  tCGACTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTAAATACg  <  2:445505/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCGCCAAAGGCACCGAGGGCATCGCGACGCGCGCTTTCACCGATCACCACCACATAGGTCTGATACTTCGGCTTAACGGCAGTGACCGTCCAGGTATCTTTCATTCCGGAAAGTTTTGCCATCCGTTCTTGCTCTTCAATCACCTCGTTATTATTGACGATGACATCCTTAGCAAAACGAAATACCGGATAGCCAGTATCTTTCAGTTTAAATACG  >  NC_000913/851810‑852103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑