Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	878,968	A→G	D76G (GAC→GGC)	yliI →	soluble aldose sugar dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	878,968	0	A	G	100.0%	192.8 / NA	53	D76G (GAC→GGC)	yliI	soluble aldose sugar dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/34);  total (19/34)

GAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGCTTAAGTGAT  >  NC_000913/878823‑879113                                                                                                                                                  |                                                                                                                                                  gAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGttt                                                                                                                                              >  1:298213/1‑151 (MQ=255)   aGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTgg                                                                                                                                            >  1:98114/1‑151 (MQ=255)         gCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCAc                                                                                                                                      <  2:784815/151‑1 (MQ=255)           aaGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACgg                                                                                                                                    <  2:793535/151‑1 (MQ=255)           aaGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACgg                                                                                                                                    <  2:477823/151‑1 (MQ=255)                 aaCTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAggg                                                                                                                              <  1:109580/151‑1 (MQ=255)                     cGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGggcg                                                                                                                            <  2:218307/149‑1 (MQ=255)                      gACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGcc                                                                                                                         <  1:972820/151‑1 (MQ=255)                        ccATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCctg                                                                                                                       <  2:468714/151‑1 (MQ=255)                               tGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACg                                                                                                                >  2:427399/1‑151 (MQ=255)                                   cACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGt                                                                                                            >  1:485157/1‑151 (MQ=255)                                    aCTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGtt                                                                                                           <  1:584954/151‑1 (MQ=255)                                    aCTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGtt                                                                                                           <  2:717344/151‑1 (MQ=255)                                      tGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGtttt                                                                                                         >  2:65474/1‑151 (MQ=255)                                          cTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAgcg                                                                                                     >  1:607649/1‑151 (MQ=255)                                            ttttACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAgcgc                                                                                                    <  1:29575/150‑1 (MQ=255)                                                   cGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGAtttt                                                                                            <  1:41343/151‑1 (MQ=255)                                                   cGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGAtttt                                                                                            <  2:640123/151‑1 (MQ=255)                                                    gATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTg                                                                                           <  1:815489/151‑1 (MQ=255)                                                      tAATCACGGCATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCCGGAAAAGGATTATCTGCGCCGCTTTCCGGAGCTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGCGGCTTTAGCGCCTGATTTTGCt                                                                                         >  2:4161/1‑151 (MQ=255)                                                       aaTCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTc                                                                                        <  2:181419/151‑1 (MQ=255)                                                        aTCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCa                                                                                       <  1:114007/151‑1 (MQ=255)                                                            cGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGtct                                                                                   <  2:752891/151‑1 (MQ=255)                                                                aTGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTcgc                                                                                <  2:512961/150‑1 (MQ=255)                                                                     aaTCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCAt                                                                           <  1:363063/150‑1 (MQ=255)                                                                        cACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTgg                                                                       <  2:662273/151‑1 (MQ=255)                                                                        cACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTgg                                                                       >  1:833149/1‑151 (MQ=255)                                                                           tctGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGtt                                                                     >  2:591476/1‑150 (MQ=255)                                                                            ctGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTa                                                                    >  1:882791/1‑150 (MQ=255)                                                                              gcgcGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGt                                                                 <  1:42484/151‑1 (MQ=255)                                                                                gcggcggcgAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTa                                                               <  1:846575/151‑1 (MQ=255)                                                                                gcggcggcgAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTa                                                               <  1:228332/151‑1 (MQ=255)                                                                                 cggcggcgAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTAt                                                              >  2:535746/1‑151 (MQ=255)                                                                                        gAGTTGCGTCACTGGCAAGGAGGAAAAGGATTAGCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGCCCTGCTGGACGTGGTTTTAGCGCATGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTAGTCCGAAg                                                       <  2:95858/151‑1 (MQ=255)                                                                                        gAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGa                                                         >  2:943146/1‑149 (MQ=255)                                                                                           ttGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTg                                                    >  1:201101/1‑151 (MQ=255)                                                                                             gCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTgg                                                   <  1:80722/150‑1 (MQ=255)                                                                                                     ggCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCgatgat                                            <  1:763226/149‑1 (MQ=255)                                                                                                     ggCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATgg                                          <  1:196475/151‑1 (MQ=255)                                                                                                             ggAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCg                                  <  2:401200/151‑1 (MQ=255)                                                                                                              gAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCgg                                 <  2:274567/151‑1 (MQ=255)                                                                                                                  aGGATTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTATCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAAct                             <  2:906944/151‑1 (MQ=255)                                                                                                                     aTTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAActgct                          <  1:418279/151‑1 (MQ=255)                                                                                                                     aTTATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAActgct                          <  1:543595/151‑1 (MQ=255)                                                                                                                       tATCTGCGCCGCTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGATTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAActgctt                         <  2:98659/150‑2 (MQ=255)                                                                                                                             cgccgcTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGtt                   >  2:676195/1‑150 (MQ=255)                                                                                                                              gccgcTTTCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTAt                 >  2:821755/1‑151 (MQ=255)                                                                                                                                     tCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGc           >  1:682751/1‑150 (MQ=255)                                                                                                                                     tCCGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGCt          >  1:264800/1‑151 (MQ=255)                                                                                                                                      ccGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGCtt         >  1:231643/1‑151 (MQ=255)                                                                                                                                       cGGAGTTCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGCtt         >  2:36232/1‑150 (MQ=255)                                                                                                                                             tCCGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGCTTAAGtga   <  1:504234/150‑1 (MQ=255)                                                                                                                                              ccGGGCGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGCTTAAGtgat  <  2:779325/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GAAGTACTGCAAGACAAACTCGACCATCCCTGGGCACTGGCCTTTTTACCCGATAATCACGGTATGTTAATCACTCTGCGCGGCGGCGAGTTGCGTCACTGGCAAGCAGGAAAAGGATTATCTGCGCCGCTTTCCGGAGTTCCGGACGTTTGGGCGCACGGGCAGGGCGGCCTGCTGGACGTGGTTTTAGCGCCTGATTTTGCTCAGTCTCGCCGCATCTGGTTAAGTTATTCCGAAGTTGGCGATGATGGCAAAGCCGGAACTGCTGTGGGTTATGGCCGCTTAAGTGAT  >  NC_000913/878823‑879113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑