Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,132,321	C→T	T156T (ACC→ACT)	flgD →	flagellar hook assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,132,321	0	C	T	100.0%	180.4 / NA	46	T156T (ACC→ACT)	flgD	flagellar hook assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/21);  total (25/21)

GCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGT  >  NC_000913/1132178‑1132465                                                                                                                                                |                                                                                                                                                 gCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCgg                                                                                                                                             >  2:470680/1‑149 (MQ=255)    ggAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTc                                                                                                                                        <  1:22623/151‑1 (MQ=255)       aCCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTcac                                                                                                                                      >  2:600122/1‑150 (MQ=255)                  agaagGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTgg                                                                                                                          >  2:143622/1‑151 (MQ=255)                   gaagGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTggg                                                                                                                         <  1:250143/151‑1 (MQ=255)                     agGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGAc                                                                                                                       <  1:352988/151‑1 (MQ=255)                          cTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCTTCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTa                                                                                                                   >  2:741686/1‑150 (MQ=255)                          cTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTAc                                                                                                                  >  2:612202/1‑151 (MQ=255)                              gaccacgaccacGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTg                                                                                                              >  2:246955/1‑151 (MQ=255)                              gaccacgaccacGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTg                                                                                                              <  1:908338/151‑1 (MQ=255)                                  acgaccacgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTg                                                                                                          >  1:605188/1‑151 (MQ=255)                                       cacgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGgc                                                                                                     <  2:103717/151‑1 (MQ=255)                                       cacgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGgc                                                                                                     >  1:591062/1‑151 (MQ=255)                                       cacgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGgc                                                                                                     >  1:762904/1‑151 (MQ=255)                                         cgCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCGCCTGGGACGGTACGTTGACTGATGgcag                                                                                                   <  1:234976/151‑1 (MQ=255)                                            cGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCAc                                                                                                <  2:336921/151‑1 (MQ=255)                                             gTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACt                                                                                               <  1:4785/151‑1 (MQ=255)                                              tttGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTg                                                                                              >  1:308496/1‑151 (MQ=255)                                                       gAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAAc                                                                                      <  1:635530/150‑1 (MQ=255)                                                            gCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCt                                                                                >  2:22155/1‑151 (MQ=255)                                                             cAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCtt                                                                               >  2:768863/1‑151 (MQ=255)                                                              aaCAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCtt                                                                               >  1:313716/1‑150 (MQ=255)                                                                        aGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCg                                                                    >  1:316371/1‑151 (MQ=255)                                                                           cAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGAtt                                                                 <  2:206874/151‑1 (MQ=255)                                                                            aaaGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGAtt                                                                 >  1:882808/1‑150 (MQ=255)                                                                               gTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAgcg                                                             <  2:268607/151‑1 (MQ=255)                                                                                    ggCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAg                                                         <  2:197493/150‑1 (MQ=255)                                                                                     gCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTa                                                       <  2:953535/151‑1 (MQ=255)                                                                                      ccaccaTCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTaa                                                      <  1:984486/151‑1 (MQ=255)                                                                                       caccaTCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAAc                                                     >  2:31451/1‑151 (MQ=255)                                                                                        accaTCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACg                                                    <  2:397961/151‑1 (MQ=255)                                                                                                                 ttGTGCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGcc                           >  2:556160/1‑151 (MQ=255)                                                                                                                   gtgCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCg                          <  1:691567/150‑1 (MQ=255)                                                                                                                    tgCGCACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGc                         >  2:872341/1‑150 (MQ=255)                                                                                                                     gcgcACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCt                        <  1:916553/150‑1 (MQ=255)                                                                                                                        cACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAg                    <  1:86181/151‑1 (MQ=255)                                                                                                                        cACCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAg                    <  1:388047/151‑1 (MQ=255)                                                                                                                         aCCATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGt                   >  2:881841/1‑151 (MQ=255)                                                                                                                          ccATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGtt                  >  1:133005/1‑151 (MQ=255)                                                                                                                           cATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGttt                 <  2:397411/151‑1 (MQ=255)                                                                                                                           cATTGATATTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGttt                 <  1:731344/151‑1 (MQ=255)                                                                                                                                atatTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGctct            >  1:391057/1‑151 (MQ=255)                                                                                                                                 tatTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTg           >  2:558739/1‑151 (MQ=255)                                                                                                                                  atTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTgg          >  1:94129/1‑151 (MQ=255)                                                                                                                                  atTGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTgg          >  2:911619/1‑151 (MQ=255)                                                                                                                                    tGGTGAACTGACTGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTg        >  1:977059/1‑151 (MQ=255)                                                                                                                                          actgactgCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGgt  <  2:114216/151‑1 (MQ=255)                                                                                                                                          actgactgCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGgt  <  1:696127/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                 GCGGGAACCGGCAGTGAAGAAGGGGCTGTGACCACGACCACGCCGTTTGGTGTTGAGCTGCAACAGGCGGCAGACAAAGTTACGGCCACCATCACCGATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGT  >  NC_000913/1132178‑1132465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑