Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,270,861 A→G V330A (GTG→GCG)  chaA ← calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,8610AG98.0% 175.4 / ‑6.7 49V330A (GTG→GCG) chaAcalcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (23/25);  minor base C (1/0);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGT  >  NC_000913/1270713‑1271005
                                                                                                                                                    |                                                                                                                                                
ctcCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGgccgcc                                                                                                                                                >  2:3131/1‑151 (MQ=255)
 tcCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGgccgcc                                                                                                                                                >  2:940866/1‑150 (MQ=255)
   ccccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATc                                                                                                                                             >  1:289590/1‑151 (MQ=255)
      cGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATcacc                                                                                                                                          >  1:433676/1‑151 (MQ=255)
        aTTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATcaccac                                                                                                                                        >  1:38716/1‑151 (MQ=255)
         ttACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCaccacc                                                                                                                                       >  1:221179/1‑151 (MQ=255)
              gTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTc                                                                                                                                  <  1:763151/151‑1 (MQ=255)
                 aTCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTgg                                                                                                                               >  2:260204/1‑151 (MQ=255)
                 aTCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTgg                                                                                                                               >  1:193077/1‑151 (MQ=255)
                    ggaggaGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGgcgc                                                                                                                            <  2:265855/151‑1 (MQ=255)
                        gagaTATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCAcc                                                                                                                         >  2:248609/1‑150 (MQ=255)
                             ataACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTg                                                                                                                    <  1:340856/150‑1 (MQ=255)
                             ataACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGc                                                                                                                   <  2:926400/151‑1 (MQ=255)
                             ataACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGc                                                                                                                   >  1:161001/1‑151 (MQ=255)
                                   gTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTg                                                                                                             <  1:647871/151‑1 (MQ=255)
                                        gCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAtt                                                                                                        <  2:404006/151‑1 (MQ=255)
                                        gCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAtt                                                                                                        <  2:345474/151‑1 (MQ=255)
                                        gCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAtt                                                                                                        >  2:698838/1‑151 (MQ=255)
                                          aaaTATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCg                                                                                                      <  1:555003/151‑1 (MQ=255)
                                             tatCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGtt                                                                                                    >  2:627156/1‑150 (MQ=255)
                                              atCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTAc                                                                                                  <  1:845953/151‑1 (MQ=255)
                                                    atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTc                                                                                             >  1:78618/1‑150 (MQ=255)
                                                           aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGc                                                                                     >  1:541222/1‑151 (MQ=255)
                                                             gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCa                                                                                    >  1:670471/1‑150 (MQ=255)
                                                                gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGTAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTa                                                                                <  1:212474/151‑1 (MQ=255)
                                                                      aGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGt                                                                            >  1:131767/1‑149 (MQ=255)
                                                                       gTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac                                                                         >  1:892632/1‑151 (MQ=255)
                                                                         gCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacg                                                                        <  1:793965/150‑1 (MQ=255)
                                                                          ccAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacgac                                                                      >  2:359089/1‑151 (MQ=255)
                                                                                       gcCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGc                                                         <  2:491504/151‑1 (MQ=255)
                                                                                         cATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGa                                                       <  2:809795/151‑1 (MQ=255)
                                                                                          aTTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGa                                                       >  1:547255/1‑150 (MQ=255)
                                                                                             gAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg                                                   <  1:994325/151‑1 (MQ=255)
                                                                                                cACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGtt                                                <  2:827348/151‑1 (MQ=255)
                                                                                                    ttAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCt                                             >  2:215724/1‑150 (MQ=255)
                                                                                                      aGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAac                                          <  1:3405/151‑1 (MQ=255)
                                                                                                       gTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAaca                                         <  2:160978/151‑1 (MQ=255)
                                                                                                         aCGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACt                                       <  1:13104/151‑1 (MQ=255)
                                                                                                            tCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGa                                     >  1:807894/1‑150 (MQ=255)
                                                                                                              cGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAAcc                                  <  2:223244/151‑1 (MQ=255)
                                                                                                                 tGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCaa                                >  1:983880/1‑150 (MQ=255)
                                                                                                                    agaAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGaa                            <  1:329090/151‑1 (MQ=255)
                                                                                                                    agaAGGAGATATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGa                             >  1:819779/1‑150 (MQ=255)
                                                                                                                          agaTATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGAtt                      <  1:359132/151‑1 (MQ=255)
                                                                                                                           gaTATGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTc                     >  2:545576/1‑151 (MQ=255)
                                                                                                                              atGGCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATc                  <  1:691435/151‑1 (MQ=255)
                                                                                                                                 gCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATcgcg               <  2:713754/151‑1 (MQ=255)
                                                                                                                                 gCACAGCACTAAAGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACACGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATcgc                >  2:824052/1‑150 (MQ=255)
                                                                                                                                        aCTAAAGAGGCCCCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGCACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCgcgcgc            >  2:275676/1‑147 (MQ=255)
                                                                                                                                            aaGAGGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTg    <  2:670400/151‑1 (MQ=255)
                                                                                                                                              gagGCCGCCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGgt  <  1:940946/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGT  >  NC_000913/1270713‑1271005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑