Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,516	A→G	V377A (GTC→GCC)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,516	0	A	G	100.0%	192.2 / NA	53	V377A (GTC→GCC)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/26);  total (27/26)

ACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGC  >  NC_000913/1307371‑1307661                                                                                                                                                  |                                                                                                                                                  aCGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAg                                                                                                                                               <  1:643421/150‑1 (MQ=255)    cATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATc                                                                                                                                           >  2:589983/1‑151 (MQ=255)     atatCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCg                                                                                                                                          >  2:837653/1‑151 (MQ=255)        tCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGt                                                                                                                                       >  1:517848/1‑151 (MQ=255)         cAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGt                                                                                                                                       <  1:137924/150‑1 (MQ=255)          aaGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTc                                                                                                                                      >  2:363417/1‑150 (MQ=255)               tAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCAtttt                                                                                                                                 <  2:110298/150‑1 (MQ=255)                aaCTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCAtttttt                                                                                                                               >  2:418671/1‑151 (MQ=255)                    gtgCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTcgcg                                                                                                                           <  1:489374/151‑1 (MQ=255)                    gtgCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTcgc                                                                                                                            <  1:665784/150‑1 (MQ=255)                                   aGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGGGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATTCTGa                                                                                                            <  2:933235/151‑1 (MQ=255)                                   aGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTg                                                                                                             >  2:610714/1‑150 (MQ=255)                                     ttCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGAc                                                                                                           >  1:367067/1‑150 (MQ=255)                                       cGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATc                                                                                                        >  1:767779/1‑151 (MQ=255)                                          cATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCAc                                                                                                     >  2:8944/1‑151 (MQ=255)                                             cGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCAcccc                                                                                                  <  1:900128/151‑1 (MQ=255)                                              gACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCcg                                                                                                 >  2:947207/1‑151 (MQ=255)                                               aCCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCAAGCATAGAGTCATTTTTTCGCGGAAGGATAATACTGACATCAACCCcgc                                                                                                >  2:43872/1‑151 (MQ=255)                                                    cACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTgc                                                                                           >  1:580009/1‑151 (MQ=255)                                                           ttGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGccgccg                                                                                    >  1:100432/1‑151 (MQ=255)                                                           ttGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGccgccg                                                                                    >  1:155302/1‑151 (MQ=255)                                                           ttGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGccgccg                                                                                    >  2:960873/1‑151 (MQ=255)                                                             ggTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTg                                                                                  <  1:433814/151‑1 (MQ=255)                                                             ggTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTg                                                                                  <  2:396124/151‑1 (MQ=255)                                                                      tAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGc                                                                          >  2:971432/1‑150 (MQ=255)                                                                            gCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGt                                                                   >  1:163778/1‑151 (MQ=255)                                                                                tCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAat                                                               >  1:309515/1‑151 (MQ=255)                                                                                  aaaCTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCCTTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCAAGAAGTAAat                                                               >  2:989737/1‑149 (MQ=255)                                                                                      tGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGc                                                         >  1:571703/1‑151 (MQ=255)                                                                                       gATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCg                                                          <  2:587611/149‑1 (MQ=255)                                                                                       gATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCg                                                          <  2:827688/149‑1 (MQ=255)                                                                                          aaaTTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTgg                                                     <  2:120257/151‑1 (MQ=255)                                                                                          aaaTTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTgg                                                     <  2:672936/151‑1 (MQ=255)                                                                                            aTTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGca                                                   <  2:398817/151‑1 (MQ=255)                                                                                                tAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACaa                                                >  1:810259/1‑150 (MQ=255)                                                                                                 aaCCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACaaa                                               <  2:146193/150‑1 (MQ=255)                                                                                                   ccccAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGt                                             >  1:736934/1‑150 (MQ=255)                                                                                                        gcagccagcagTTCCGTAAAGAATGCGCGTCTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGTACATAGTAGTGc                                        <  2:960023/150‑1 (MQ=255)                                                                                                        gcagccagcagTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCg                                       <  1:406178/151‑1 (MQ=255)                                                                                                                  gTTCCGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGAt                             <  2:481401/151‑1 (MQ=255)                                                                                                                     ccGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCa                           >  1:913703/1‑150 (MQ=255)                                                                                                                     ccGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCa                           <  2:65888/150‑1 (MQ=255)                                                                                                                      cGTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCaa                          >  1:159509/1‑150 (MQ=255)                                                                                                                       gTAAAGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAat                        >  1:329232/1‑151 (MQ=255)                                                                                                                           aGAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTc                    <  1:539642/151‑1 (MQ=255)                                                                                                                            gAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCa                   <  2:696288/151‑1 (MQ=255)                                                                                                                            gAATGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCa                   <  2:503834/151‑1 (MQ=255)                                                                                                                               tGCGCGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTAACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCa                   <  2:787698/148‑1 (MQ=255)                                                                                                                                gcgcGACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCAcgcg               <  1:718954/151‑1 (MQ=255)                                                                                                                                    gACTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCg            >  2:910186/1‑150 (MQ=255)                                                                                                                                      cTGGCCCAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAAt         >  1:416049/1‑151 (MQ=255)                                                                                                                                          cccAGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGc     <  1:418719/151‑1 (MQ=255)                                                                                                                                             aGCCGGCCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCGCCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGc  <  2:95189/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCGCGGAAGGATAATACTGACATCCACCCCGCGCTGCGCCGCCGTGCAAATCGCATGAAGTAAATCATCGCTTGGCACAAAGTAGGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGC  >  NC_000913/1307371‑1307661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑