Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,407,641	A→G	V52A (GTC→GCC)	ydaM ←	diguanylate cyclase, csgD regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,407,641	0	A	G	100.0%	223.5 / NA	62	V52A (GTC→GCC)	ydaM	diguanylate cyclase, csgD regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/32);  total (30/32)

ACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGACGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGT  >  NC_000913/1407494‑1407782                                                                                                                                                    |                                                                                                                                              aCCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCgg                                                                                                                                            >  2:462673/1‑151 (MQ=255)        ctgtttctgtCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAggg                                                                                                                                     >  1:471587/1‑151 (MQ=255)          gtttctgtCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTc                                                                                                                                   <  2:162889/151‑1 (MQ=255)            ttctgtCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCg                                                                                                                                 >  2:817953/1‑151 (MQ=255)               tgtCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGtt                                                                                                                              >  1:115897/1‑151 (MQ=255)                      tttCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCt                                                                                                                       >  1:862076/1‑151 (MQ=255)                      tttCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCt                                                                                                                       >  2:13770/1‑151 (MQ=255)                       ttCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCt                                                                                                                       <  2:569647/150‑1 (MQ=255)                       ttCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTg                                                                                                                      >  2:154534/1‑151 (MQ=255)                          gCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACt                                                                                                                   >  2:110365/1‑151 (MQ=255)                          gCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACt                                                                                                                   >  1:391847/1‑151 (MQ=255)                           cAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTa                                                                                                                  <  1:178367/151‑1 (MQ=255)                            aaaaCAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTa                                                                                                                  >  2:451698/1‑150 (MQ=255)                             aaaCAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTaaa                                                                                                                <  1:31436/151‑1 (MQ=255)                                 aaGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTg                                                                                                            >  1:22131/1‑151 (MQ=255)                                  aGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGa                                                                                                           <  1:84268/151‑1 (MQ=255)                                  aGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGa                                                                                                           <  2:824678/151‑1 (MQ=255)                                      gACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTc                                                                                                       >  1:645124/1‑151 (MQ=255)                                         aGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCtt                                                                                                     <  1:669246/150‑1 (MQ=255)                                         aGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTg                                                                                                    >  2:380778/1‑151 (MQ=255)                                          gCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGc                                                                                                   <  1:709263/151‑1 (MQ=255)                                              aaTGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCa                                                                                               <  1:283796/151‑1 (MQ=255)                                                  cTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAAc                                                                                             >  1:963378/1‑149 (MQ=255)                                                  cTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACa                                                                                            <  2:356166/150‑1 (MQ=255)                                                      ttCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGttt                                                                                        <  2:245123/150‑1 (MQ=255)                                                       tCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTcg                                                                                      >  2:511271/1‑151 (MQ=255)                                                           cttcttTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTcgcc                                                                                    <  2:748805/149‑1 (MQ=255)                                                                ttACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTa                                                                              <  1:920902/150‑1 (MQ=255)                                                                      tGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCAt                                                                       >  1:450755/1‑151 (MQ=255)                                                                       gAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCa                                                                        <  1:722702/149‑1 (MQ=255)                                                                        aaCAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATaa                                                                     <  2:286397/151‑1 (MQ=255)                                                                        aaCAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATa                                                                      >  1:317291/1‑150 (MQ=255)                                                                          cAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAAt                                                                   <  1:742528/151‑1 (MQ=255)                                                                            gTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTa                                                                 <  1:691404/151‑1 (MQ=255)                                                                                ggATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTg                                                             <  2:431098/151‑1 (MQ=255)                                                                                ggATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTg                                                             <  1:709845/151‑1 (MQ=255)                                                                                 gATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGt                                                            <  2:383458/151‑1 (MQ=255)                                                                                       gATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTc                                                       >  2:385256/1‑150 (MQ=255)                                                                                         taataTCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCaa                                                     >  2:871354/1‑150 (MQ=255)                                                                                           atatCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAgg                                                  <  1:318279/151‑1 (MQ=255)                                                                                           atatCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAgg                                                  >  2:513568/1‑151 (MQ=255)                                                                                           atatCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAgg                                                  >  1:773911/1‑151 (MQ=255)                                                                                             atCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAggg                                                 <  1:713614/150‑1 (MQ=255)                                                                                                            cAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACa                                 <  2:548673/151‑1 (MQ=255)                                                                                                             aGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGAc                                  >  2:511012/1‑149 (MQ=255)                                                                                                                  ggAGAGGTATTTCGGCAGTTCTTTTTGGGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGTCTAAACGTGAGGTCTAGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGCGCGAAACGATCCAGACa                                 >  2:322056/1‑145 (MQ=255)                                                                                                                   gagaGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTgg                          <  2:857192/151‑1 (MQ=255)                                                                                                                   gagaGGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTgg                          >  1:399917/1‑151 (MQ=255)                                                                                                                    agagGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGt                         <  2:585362/151‑1 (MQ=255)                                                                                                                     gagGTATTTCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGt                         <  1:547077/150‑1 (MQ=255)                                                                                                                            ttCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCaaa                                                    >  2:382244/1‑116 (MQ=255)                                                                                                                            ttCGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCaaa                                                    <  1:382288/116‑1 (MQ=255)                                                                                                                              cGGCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTcc               >  2:735488/1‑151 (MQ=255)                                                                                                                                gCAGTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTcc               >  2:139216/1‑149 (MQ=255)                                                                                                                                   gTTCTTTTTGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAggg           >  1:324273/1‑150 (MQ=255)                                                                                                                                        ttttGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTAt        <  2:167113/148‑1 (MQ=255)                                                                                                                                        ttttGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTg      <  2:108845/150‑1 (MQ=255)                                                                                                                                        ttttGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTg      <  1:632217/150‑1 (MQ=255)                                                                                                                                         tttGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTg      >  2:421219/1‑149 (MQ=255)                                                                                                                                         tttGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGa     >  1:777922/1‑150 (MQ=255)                                                                                                                                          ttGTGAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGaa    <  1:414493/150‑1 (MQ=255)                                                                                                                                            gtgAGGAGGCGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGt  <  1:566920/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              ACCGGTTCTGTTTCTGTCAGCTTTCGCAAAACAAGCCGACAGCTCAATGCTGTTTCCTCTTCTTTACGCTGAACAGTGAGGATTTCGATAATATCGTGTTGGTTTTGCAGATCGGAGAGGTATTTCGGCAGTTCTTTTTGTGAGGAGACGGAATAGGGTCCGGTTCGTAGCTGACTAAACGTGAGGTCTTGCATCAACAGTTTCGCCGCGCTATTGGCATAAATTAACTGTTCCTCAAAGGGCGAAACGATCCAGACAGGACTGGTGAGTAAGTCCAGGGTATTGAAGT  >  NC_000913/1407494‑1407782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑