Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,444,706	C→T	G552G (GGC→GGT)	ydbH →	putative membrane‑anchored protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,444,706	0	C	T	100.0%	176.6 / NA	45	G552G (GGC→GGT)	ydbH	putative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/27);  total (18/27)

TTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGC  >  NC_000913/1444561‑1444852                                                                                                                                                  |                                                                                                                                                   ttAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCgg                                                                                                                                                <  1:974262/150‑1 (MQ=255)    gcggcgCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTc                                                                                                                                             <  2:328487/150‑1 (MQ=255)    gcggcgCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCg                                                                                                                                            <  1:789868/151‑1 (MQ=255)       gcgcgcTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGt                                                                                                                                         <  2:393123/151‑1 (MQ=255)                cACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTc                                                                                                                                 <  2:990472/150‑1 (MQ=255)                      aCGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGa                                                                                                                          <  2:829268/151‑1 (MQ=255)                      aCGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCACCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGGGAGATTGGTCCGGTTCGGGTAAATGGGCGCTggg                                                                                                                           >  2:78932/1‑150 (MQ=255)                        gCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACg                                                                                                                        <  1:26492/151‑1 (MQ=255)                        gCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACg                                                                                                                        >  1:908494/1‑151 (MQ=255)                                    cTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTgc                                                                                                            >  1:975073/1‑151 (MQ=255)                                      gTTCACTGGCGGCATTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTgc                                                                                                            <  2:49808/149‑1 (MQ=255)                                          aCTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTa                                                                                                      <  1:208293/151‑1 (MQ=255)                                             ggcggcAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTaa                                                                                                     <  1:964674/149‑1 (MQ=255)                                              gcggcAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGc                                                                                                  <  2:867706/151‑1 (MQ=255)                                                      gtgtTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCtggtgg                                                                                           <  1:375823/150‑1 (MQ=255)                                                      gtgtTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCtggtgg                                                                                           <  2:267781/150‑1 (MQ=255)                                                       tgtTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGcc                                                                                         >  1:136160/1‑151 (MQ=255)                                                         ttACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCt                                                                                        >  1:949025/1‑150 (MQ=255)                                                          tACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTa                                                                                       >  2:483619/1‑150 (MQ=255)                                                          tACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATATACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTa                                                                                       <  2:417363/150‑1 (MQ=255)                                                                cATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATc                                                                                >  2:142038/1‑151 (MQ=255)                                                                   cAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCAc                                                                              <  2:24932/150‑1 (MQ=255)                                                                    aaCCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTg                                                                            >  2:831860/1‑151 (MQ=255)                                                                      ccTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGAc                                                                          <  2:854260/151‑1 (MQ=255)                                                                         tAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGt                                                                       >  2:430479/1‑151 (MQ=255)                                                                          aaaaTTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGt                                                                       >  2:561443/1‑150 (MQ=255)                                                                           aaaTTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTAt                                                                     >  1:506710/1‑151 (MQ=255)                                                                                              gcgATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCT‑GTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCAccc                                                 <  1:921372/151‑1 (MQ=255)                                                                                                gATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCg                                                >  1:956989/1‑151 (MQ=255)                                                                                                   cctacctaTTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACt                                             >  1:45960/1‑151 (MQ=255)                                                                                                   cctacctaTTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGCATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACt                                             >  1:417552/1‑151 (MQ=255)                                                                                                          aTTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAg                                        <  1:219016/149‑1 (MQ=255)                                                                                                             tCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGa                                    <  1:470349/150‑1 (MQ=255)                                                                                                              ccTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAAc                                  >  1:227866/1‑151 (MQ=255)                                                                                                                  tttAAAGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTAc                              >  1:675375/1‑151 (MQ=255)                                                                                                                  tttAAAGGCGATTTACATGCAGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACtt                                <  2:449879/149‑1 (MQ=255)                                                                                                                       aGGCGATTTACATGCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGAt                         <  2:105776/151‑1 (MQ=255)                                                                                                                                    gCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTAt             <  1:497480/150‑1 (MQ=255)                                                                                                                                    gCTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTAt             <  1:236227/150‑1 (MQ=255)                                                                                                                                     cTGGTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGc           >  1:340560/1‑151 (MQ=255)                                                                                                                                       ggTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCt          <  1:105603/150‑1 (MQ=255)                                                                                                                                       ggTGAGATTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTc         <  2:908414/151‑1 (MQ=255)                                                                                                                                          gagaTTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAgg      <  2:606239/151‑1 (MQ=255)                                                                                                                                            gaTTGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGtt    >  1:917317/1‑151 (MQ=255)                                                                                                                                               tGGTCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGc  <  1:432136/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TTAGCGGCGCGCTGTCACTGGACGCCGGGCAAACGCTGTTCACTGGCGGCAGTGTGTTACCGCCATCAACCTTAAAATTTAGCGTCGATGGGCGCGATCCTACCTATTTCCTCTTTAAAGGCGATTTACATGCTGGTGAGATTGGCCCGGTTCGGGTAAATGGTCGCTGGGACGGTATTCGTCTGCGCGGTAACGCCTGGTGGCCTAAACAATCACTGACCGTATTCCAGCCGCTGGTGCCACCCGACTGGAAGATGAACTTACGCGATGGTGAACTGTATGCTCAGGTTGC  >  NC_000913/1444561‑1444852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑