Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	187,251	G→A	T467M (ACG→ATG)	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	187,251	0	G	A	100.0%	174.8 / NA	46	T467M (ACG→ATG)	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

ATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTT  >  NC_000913/187104‑187396                                                                                                                                                    |                                                                                                                                                  atatCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCatgt                                                                                                                                                <  1:509066/151‑1 (MQ=255) atatCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGACAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCatg                                                                                                                                                 >  2:457092/1‑150 (MQ=255)   atCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTa                                                                                                                                               <  2:812866/150‑1 (MQ=255)     cGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAgg                                                                                                                                             <  2:559778/150‑1 (MQ=255)     cGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGc                                                                                                                                            >  2:120061/1‑151 (MQ=255)      gTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACTTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCg                                                                                                                                           <  2:423224/151‑1 (MQ=255)                 cggcgATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATc                                                                                                                                <  2:159664/151‑1 (MQ=255)                    cgATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCaaa                                                                                                                             <  2:520192/151‑1 (MQ=255)                      aTGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACt                                                                                                                           <  2:999591/151‑1 (MQ=255)                      aTGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACt                                                                                                                           <  2:957357/151‑1 (MQ=255)                          aaaTCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCAt                                                                                                                       >  1:28421/1‑151 (MQ=255)                          aaaTCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCAt                                                                                                                       >  2:804604/1‑151 (MQ=255)                                  tCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCcg                                                                                                                >  1:204305/1‑150 (MQ=255)                                   cAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGccct                                                                                                                <  1:192666/149‑2 (MQ=255)                                   cAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCcgac                                                                                                              >  2:22715/1‑151 (MQ=255)                                   cAGTTCACGGCAGGCGCGGCCAGACGTCCACACACATCGGATGGCGCTGGCGCGTTTATTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTCGGCGTGGACCCGATCAAACTGCATCTGCCcga                                                                                                               >  2:354715/1‑150 (MQ=255)                                    aGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCcgacg                                                                                                             >  1:198435/1‑151 (MQ=255)                                      ttGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGat                                                                                                           >  1:723047/1‑151 (MQ=255)                                       tGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGat                                                                                                           <  1:499334/150‑1 (MQ=255)                                       tGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGat                                                                                                           <  2:978520/150‑1 (MQ=255)                                        gACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGata                                                                                                          <  2:196950/150‑1 (MQ=255)                                              aGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGAcc                                                                                                   >  1:406919/1‑151 (MQ=255)                                                  gcgGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTg                                                                                               >  1:479625/1‑151 (MQ=255)                                                     gCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCgg                                                                                            <  2:350602/151‑1 (MQ=255)                                                     gCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCgg                                                                                            <  2:821797/151‑1 (MQ=255)                                                            gTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATAc                                                                                     >  2:658449/1‑151 (MQ=255)                                                               cacacacaACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCAtata                                                                                      >  2:107762/1‑147 (MQ=255)                                                                     caACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGc                                                                            >  2:970404/1‑151 (MQ=255)                                                                      aaCGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAg                                                                             >  2:809230/1‑149 (MQ=255)                                                                          gATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGc                                                                        >  2:364093/1‑150 (MQ=255)                                                                          gATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCt                                                                       <  2:336407/151‑1 (MQ=255)                                                                              gcgctggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATgg                                                                   <  2:112991/151‑1 (MQ=255)                                                                                 ctggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCg                                                                 >  1:15052/1‑150 (MQ=255)                                                                                  tggcgcGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGAt                                                               >  2:528530/1‑151 (MQ=255)                                                                                      gcgTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCAt                                                           <  1:681735/151‑1 (MQ=255)                                                                                              tcACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCa                                                      <  2:583187/148‑1 (MQ=255)                                                                                               cACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATa                                                   <  2:722969/150‑1 (MQ=255)                                                                                                      aaaaCTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCcgcg                                            <  1:639190/150‑1 (MQ=255)                                                                                                           tctcCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCAc                                      >  2:175012/1‑151 (MQ=255)                                                                                                              ccAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGc                                   >  2:895653/1‑151 (MQ=255)                                                                                                                    tCAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCggg                             >  1:798531/1‑151 (MQ=255)                                                                                                                     cAGCATCACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCggg                             <  1:364135/150‑1 (MQ=255)                                                                                                                           cACGCGGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACg                       <  2:386322/150‑1 (MQ=255)                                                                                                                             cgcgGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGc                      >  2:633502/1‑149 (MQ=255)                                                                                                                              gcgGATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGa                   <  2:198413/151‑1 (MQ=255)                                                                                                                                ggATAGTATGTTCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAAt                 <  2:851173/151‑1 (MQ=255)                                                                                                                                           tCATCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAAc       >  2:439642/1‑150 (MQ=255)                                                                                                                                             aTCCACCATGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGt    <  2:515538/151‑1 (MQ=255)                                                                                                                                               ccaccaTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGttt  <  2:471038/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGACGTCCACACACAACGGATGGCGCTGGCGCGTTTCTTCACTGGCAAAACTCTCCAGTTTCAGCATCACGCGGATAGTATGTTCATCCACCGTGTAGGCGTGGAACAGATCAAACTGCATCTGCCCGACGATATGCGACCATTGCGGCATATACGCGCCGAGCACGCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTT  >  NC_000913/187104‑187396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑