Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,494,068	A→G	E533E (GAA→GAG)	trg →	methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,494,068	0	A	G	100.0%	206.6 / NA	60	E533E (GAA→GAG)	trg	methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/29);  total (31/29)

GATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCAT  >  NC_000913/1493921‑1494212                                                                                                                                                    |                                                                                                                                                 gATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGcc                                                                                                                                                >  2:202069/1‑150 (MQ=255) gATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCt                                                                                                                                               <  2:921994/151‑1 (MQ=255) gATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGGATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGcc                                                                                                                                                >  1:878906/1‑150 (MQ=255)   tttCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGGTGGTTTCCGTCTCCACAAACATTCTGTGGCGGCAGAGCCTc                                                                                                                                              >  1:237672/1‑150 (MQ=255)   tttCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTcg                                                                                                                                             >  1:982617/1‑151 (MQ=255)    ttCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTcg                                                                                                                                             >  1:59398/1‑150 (MQ=255)        gAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCgg                                                                                                                                          >  1:677368/1‑149 (MQ=255)         aaaTGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGc                                                                                                                                       <  1:298226/151‑1 (MQ=255)         aaaTGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGGGGATGTATTCCGTCTTCACAAACATTTTGTGTCGGCAGAGCCTTGGGGAg                                                                                                                                        >  2:118282/1‑150 (MQ=255)           aTGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGGTGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCgg                                                                                                                                     >  1:324663/1‑151 (MQ=255)           aTGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGGTGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCgg                                                                                                                                     >  1:720056/1‑151 (MQ=255)            tGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCggg                                                                                                                                    >  1:881999/1‑151 (MQ=255)                 aaGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGaa                                                                                                                                >  1:989907/1‑150 (MQ=255)                       aCGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCagtta                                                                                                                         <  2:285894/151‑1 (MQ=255)                            aCAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTc                                                                                                                    <  2:421950/151‑1 (MQ=255)                               gAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCt                                                                                                                 <  2:37719/151‑1 (MQ=255)                                   gCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACgg                                                                                                             <  2:526333/151‑1 (MQ=255)                                   gCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGCGGATGTATTCCGTCTGCACAAACATTCTGGGTCGGCAGAGCCCCGCGGAGCGGGTGGACCCGTTAGTTTCGCTACgg                                                                                                             >  2:682990/1‑151 (MQ=255)                                     ttCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGgtg                                                                                                           >  1:333805/1‑151 (MQ=255)                                        tctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGtgtg                                                                                                         >  1:133340/1‑150 (MQ=255)                                         ctGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGa                                                                                                        >  2:648827/1‑150 (MQ=255)                                           ggTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGaaa                                                                                                      <  1:444172/150‑1 (MQ=255)                                            gTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGGTCCGGTGTGAAAAt                                                                                                    <  2:757013/151‑1 (MQ=255)                                              agaagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGt                                                                                                  <  2:813266/151‑1 (MQ=255)                                               gaagaGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGtt                                                                                                 <  2:358351/151‑1 (MQ=255)                                                    ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAagg                                                                                            <  2:541937/151‑1 (MQ=255)                                                    ggCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTAGCTACGGTGTGAAAATGTTCAagg                                                                                            >  1:820121/1‑151 (MQ=255)                                                      ccTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGGTGTATTCCGGCTGCCCAAACATTCTGTGTCGGCAGAGGCTCCCGGAGCGGGTGAACCAGTTAGTTTCGCCACGGTGAGAAAATGTTCaa                                                                                              >  2:873530/1‑147 (MQ=255)                                                       cTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGGTGTATTCCGTCTGCACAAACATTCTGTGTCGGCCGAGCCCCGCGGAGCGGGGGAACCAGTTAGTTTCGCTACGGGGTGAAAATGTTCAaggagg                                                                                         >  2:18600/1‑151 (MQ=255)                                                          aGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGAt                                                                                      <  2:653600/151‑1 (MQ=255)                                                              gcggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGAc                                                                                  <  1:263117/151‑1 (MQ=255)                                                               cggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACa                                                                                 <  2:486607/151‑1 (MQ=255)                                                               cggcggTGTCCCTAGAAGAACAGGCGTCACGATTAACTGAGGAGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAACGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACa                                                                                 <  2:560466/151‑1 (MQ=255)                                                                ggcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGGGTGAAAATGTTCAAGGAGGGATCg                                                                                    >  1:582624/1‑147 (MQ=255)                                                                 gcggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGa                                                                               <  1:738577/151‑1 (MQ=255)                                                                  cggTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGGCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGAc                                                                                  >  1:807088/1‑147 (MQ=255)                                                                     tgtCCCTTGAAGAACAGCCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGGGAAACCGTTAGATTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGAAcc                                                                            >  1:835219/1‑150 (MQ=255)                                                                       tCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCtt                                                                         >  1:510911/1‑151 (MQ=255)                                                                         ccTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTc                                                                        <  2:237644/150‑1 (MQ=255)                                                                              aagaaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCttt                                                                  >  2:895999/1‑151 (MQ=255)                                                                                 aaCAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAg                                                               <  2:285007/151‑1 (MQ=255)                                                                                            cGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGa                                                    <  2:320478/151‑1 (MQ=255)                                                                                                 aaCTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGAtttt                                                >  2:238995/1‑150 (MQ=255)                                                                                                     gAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGaa                                            <  2:719979/150‑1 (MQ=255)                                                                                                     gAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGaa                                            <  1:403834/150‑1 (MQ=255)                                                                                                      aGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAAt                                           >  2:818747/1‑150 (MQ=255)                                                                                                       ggCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAg                                         <  2:347426/151‑1 (MQ=255)                                                                                                             ggATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTa                                    >  1:88187/1‑150 (MQ=255)                                                                                                                 gTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAatcat                               <  1:238182/151‑1 (MQ=255)                                                                                                                   aTTCCGTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAatcatc                              >  1:998935/1‑150 (MQ=255)                                                                                                                        gTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTgg                        >  1:814015/1‑151 (MQ=255)                                                                                                                        gTCTGCACAAACATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTgg                        <  1:74539/151‑1 (MQ=255)                                                                                                                                 aaCATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCagaa               <  1:309559/151‑1 (MQ=255)                                                                                                                                  aCATTCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCaga                >  1:151693/1‑149 (MQ=255)                                                                                                                                     ttCTGTGTCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCagaag              >  2:120993/1‑148 (MQ=255)                                                                                                                                         gtgtCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGaa          <  2:794782/148‑1 (MQ=255)                                                                                                                                         gtgtCGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGaa          <  1:68841/148‑1 (MQ=255)                                                                                                                                             cGGCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGc    <  1:868422/150‑1 (MQ=255)                                                                                                                                              ggCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCAt  >  2:16005/1‑151 (MQ=255)                                                                                                                                              ggCAGAGCCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCAt  <  1:202090/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GATTTCTGAAATGGATAAGGTGACGCAACAGAATGCTTCTCTGGTAGAAGAGGCCTCAGCGGCGGCGGTGTCCCTTGAAGAACAGGCGGCACGATTAACTGAGGCGGTGGATGTATTCCGTCTGCACAAACATTCTGTGTCGGCAGAACCTCGCGGAGCGGGTGAACCAGTTAGTTTCGCTACGGTGTGAAAATGTTCAAGGAGGGATCGACAGATCCCTTCACCTTTCAGAACGGCATTGATTTTCGAATAGCGTTAATCATCAACTGGCAACCAGAAGAGAACGTCGCAT  >  NC_000913/1493921‑1494212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑