Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,710,621	G→A	D140N (GAC→AAC)	rsxG →	SoxR iron‑sulfur cluster reduction factor component; putative membrane protein of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,710,621	0	G	A	100.0%	188.1 / NA	51	D140N (GAC→AAC)	rsxG	SoxR iron‑sulfur cluster reduction factor component; putative membrane protein of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/22);  total (29/22)

AGCAACCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGAT  >  NC_000913/1710482‑1710768                                                                                                                                            |                                                                                                                                                    aGCAACCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCAcc                                                                                                                                          <  1:309378/151‑1 (MQ=255)   cAACCGCGCCAGATGTCTATTCCGGTGCGATTCAGCTGCTGGTGAGAGCCGATTTTAACGTCTCGGTACTTGGCACGCGCGTGTCAGAGCACCACGAAACGCCAGTGCTTGGCGCTAAAATCGAACTGCGCCTTTCTAACTGGATCACCCa                                                                                                                                        <  2:779943/151‑1 (MQ=255)    aaCCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCAt                                                                                                                                       >  2:346799/1‑151 (MQ=255)          gcCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCg                                                                                                                                 >  2:747687/1‑151 (MQ=255)                tGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTaa                                                                                                                            >  2:73368/1‑150 (MQ=255)                    tATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTaaaaa                                                                                                                         >  2:450537/1‑149 (MQ=255)                       tCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTaaaaaaa                                                                                                                       <  1:989696/148‑1 (MQ=255)                             gCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAgtggtg                                                                                                              <  1:936771/151‑1 (MQ=255)                             gCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAgtggtg                                                                                                              <  2:640836/151‑1 (MQ=255)                                aTTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGc                                                                                                             >  1:890488/1‑149 (MQ=255)                                aTTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAg                                                                                                           <  1:830696/151‑1 (MQ=255)                                     gcggctgGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACGGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAgatga                                                                                                       <  2:335566/147‑1 (MQ=255)                                        gctgGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATgcg                                                                                                   >  2:218789/1‑151 (MQ=255)                                          tggtggGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATgcgc                                                                                                  >  1:824630/1‑150 (MQ=255)                                            gtggGAGCCGATTTTAACGGCACTGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATATAATCGAACTGCGCCGTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATgcgc                                                                                                  <  2:472793/148‑1 (MQ=255)                                            gtggGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACt                                                                                               <  2:363665/151‑1 (MQ=255)                                                      aTTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTgaa                                                                                     <  1:956277/151‑1 (MQ=255)                                                      aTTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTga                                                                                      >  2:944509/1‑150 (MQ=255)                                                         ttAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTgaaga                                                                                   >  2:232224/1‑150 (MQ=255)                                                          tAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTgaagaa                                                                                  >  2:817094/1‑150 (MQ=255)                                                           aaCGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTgaagaa                                                                                  >  2:838566/1‑149 (MQ=255)                                                                 aCGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGAtggtg                                                                          >  1:248340/1‑151 (MQ=255)                                                                   ggTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATggtggt                                                                        <  1:450590/151‑1 (MQ=255)                                                                    ggaCTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGgtggtg                                                                       >  2:756280/3‑151 (MQ=255)                                                                        ttGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGAttt                                                                   >  2:294578/1‑151 (MQ=255)                                                                            cACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGAc                                                               >  2:233143/1‑151 (MQ=255)                                                                             aCGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGAcc                                                              >  1:471474/1‑151 (MQ=255)                                                                              cgcgcgTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGAcc                                                              >  2:791334/1‑150 (MQ=255)                                                                              cgcgcgTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCa                                                             <  1:463325/151‑1 (MQ=255)                                                                                  cgTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTc                                                         <  2:929022/151‑1 (MQ=255)                                                                                    tGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCAc                                                       >  1:464450/1‑151 (MQ=255)                                                                                      aCAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCg                                                     <  2:525284/151‑1 (MQ=255)                                                                                         gagCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGgcg                                                  <  1:204514/151‑1 (MQ=255)                                                                                                   aaaCGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTAc                                        >  2:207283/1‑151 (MQ=255)                                                                                                       gCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTccc                                    >  1:666533/1‑151 (MQ=255)                                                                                                        ccAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTcccc                                   >  1:297723/1‑151 (MQ=255)                                                                                                            ggCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCcgcgc                               <  1:233172/151‑1 (MQ=255)                                                                                                             gCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCTGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCGCCGGCGCGACGATTACTCCCcgct                                <  2:706556/149‑2 (MQ=255)                                                                                                             gCTTGGCGATAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGTTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCcgcgcg                              >  1:109238/1‑151 (MQ=255)                                                                                                                     aTAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAGGATGGCGATGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGCGGtt                        >  1:71122/1‑149 (MQ=255)                                                                                                                     aTAAAATCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTa                       >  2:860337/1‑150 (MQ=255)                                                                                                                        aaaTCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGc                   >  2:489194/1‑151 (MQ=255)                                                                                                                          aTCGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCgg                 >  1:382880/1‑151 (MQ=255)                                                                                                                            cGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGTTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTa               <  2:109224/151‑1 (MQ=255)                                                                                                                            cGAACTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGt                >  2:801373/1‑150 (MQ=255)                                                                                                                              aaCTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTaaa             <  1:454704/151‑1 (MQ=255)                                                                                                                              aaCTGCGCCTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTaaa             <  1:191327/151‑1 (MQ=255)                                                                                                                                     ccTTTCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGcc      >  2:289864/1‑151 (MQ=255)                                                                                                                                        ttCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACgcgc       <  1:747771/147‑1 (MQ=255)                                                                                                                                        ttCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCg     <  1:864994/149‑1 (MQ=255)                                                                                                                                         tCTAACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGAt  >  1:759179/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                    AGCAACCGCGCCAGATGGCTATTCCGGTGCGATTCAGCTGCTGGTGGGAGCCGATTTTAACGGCACGGTACTTGGCACGCGCGTGACAGAGCACCACGAAACGCCAGGGCTTGGCGATAAAATCGAACTGCGCCTTTCTGACTGGATCACCCATTTTGCGGGTAAAAAAATCAGTGGTGCAGATGATGCGCACTGGGCGGTGAAGAAAGATGGTGGTGATTTCGACCAGTTCACCGGCGCGACGATTACTCCCCGCGCGGTGGTTAATGCGGTAAAACGCGCCGGAT  >  NC_000913/1710482‑1710768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑