Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,070	A→G	I178T (ATT→ACT)	anmK ←	anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,070	0	A	G	100.0%	174.5 / NA	46	I178T (ATT→ACT)	anmK	anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

TTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCAC  >  NC_000913/1718926‑1719211                                                                                                                                                 |                                                                                                                                              ttttGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGc                                                                                                                                          <  1:892034/150‑1 (MQ=255)  tttGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGc                                                                                                                                          >  2:585635/1‑149 (MQ=255)    tGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGt                                                                                                                                       >  1:199841/1‑150 (MQ=255)     gcagcaGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTg                                                                                                                                      >  2:561519/1‑150 (MQ=255)                agaATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCg                                                                                                                           >  1:191609/1‑150 (MQ=255)                  aaTAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGAt                                                                                                                         <  1:623979/150‑1 (MQ=255)                         ttACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCa                                                                                                                  <  2:496369/150‑1 (MQ=255)                             ccgcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATAtt                                                                                                             >  1:799785/1‑151 (MQ=255)                             ccgcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATAtt                                                                                                             >  1:485267/1‑151 (MQ=255)                               gcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAatat                                                                                                              >  1:136547/1‑148 (MQ=255)                               gcccgTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGa                                                                                                           <  2:199819/151‑1 (MQ=255)                                      cccACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCAt                                                                                                    <  2:148202/151‑1 (MQ=255)                                          cTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcg                                                                                                 <  2:347716/150‑1 (MQ=255)                                           tCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcgtc                                                                                               >  1:621635/1‑151 (MQ=255)                                            cGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcgtc                                                                                               <  2:779872/150‑1 (MQ=255)                                            cGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATtcgtc                                                                                               <  2:180680/150‑1 (MQ=255)                                               cATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTc                                                                                           <  2:530824/151‑1 (MQ=255)                                               cATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTc                                                                                           <  2:902400/151‑1 (MQ=255)                                                aTCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCg                                                                                          >  1:168701/1‑151 (MQ=255)                                                    ttATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCgg                                                                                         <  1:968706/148‑1 (MQ=255)                                                       tCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTgg                                                                                     <  1:478040/149‑1 (MQ=255)                                                        cGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTg                                                                                  >  2:439426/1‑151 (MQ=255)                                                         gTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTg                                                                                  >  1:834680/1‑150 (MQ=255)                                                          tAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTg                                                                                  >  2:563236/1‑149 (MQ=255)                                                               tttACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCcagc                                                                           >  1:64268/1‑151 (MQ=255)                                                                  aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCcagcag                                                                         <  1:693521/150‑1 (MQ=255)                                                                  aCCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCagcagc                                                                        >  2:953498/1‑151 (MQ=255)                                                                       ccTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatg                                                                   >  2:628453/1‑151 (MQ=255)                                                                        cTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatg                                                                   >  1:431153/1‑150 (MQ=255)                                                                          gACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCatgatg                                                                >  1:917051/1‑151 (MQ=255)                                                                           aCGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATgg                                                               >  1:999938/1‑151 (MQ=255)                                                                                     cAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTa                                                     <  2:152607/151‑1 (MQ=255)                                                                                         catccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAg                                                 <  1:475186/151‑1 (MQ=255)                                                                                            ccatcAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCg                                               <  1:569595/150‑1 (MQ=255)                                                                                                cAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTgcg                                          >  2:79101/1‑151 (MQ=255)                                                                                                               aCCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCa                             >  2:253914/1‑149 (MQ=255)                                                                                                                ccGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAgg                          >  2:956205/1‑151 (MQ=255)                                                                                                                 cGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGc                         <  2:209678/151‑1 (MQ=255)                                                                                                                 cGGTATCGTAGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGc                         <  2:746989/151‑1 (MQ=255)                                                                                                                          aGCCCCCAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATcgcg                <  2:784789/151‑1 (MQ=255)                                                                                                                            cccccAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGa               <  2:318644/150‑1 (MQ=255)                                                                                                                            cccccAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGAc              >  1:294655/1‑151 (MQ=255)                                                                                                                             ccccAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACg             <  2:739407/151‑1 (MQ=255)                                                                                                                              cccAACCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACgg            <  2:323599/151‑1 (MQ=255)                                                                                                                                  aCCGGCTGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGaa        <  2:64262/151‑1 (MQ=255)                                                                                                                                        tGCCCAGGAGTGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCAc  >  1:286516/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                              TTTTGCAGCAGTGGGAGAATAACTTTACCCGCCCGTGCCCACTCGGCATCTTTATCGTAAGGTTTACCGGCCTGACGCCAGATCCAGGCATCCATCAGCATGTTACCAGGACCGGTATCGTAGCCCCCAACCGGCTGCCCAGGAATGAGCAGTGACAGATTGGCGATGCCGCCAATATTGAGCACCATTCGTCGCTCGGTTGGGTGAGCCAGCAGCGCATGATGGAACGCAGGTACCAGCGGTGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCAC  >  NC_000913/1718926‑1719211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑