Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,081,148	T→C	S39G (AGC→GGC)	sbmC ←	DNA gyrase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,148	0	T	C	100.0%	218.0 / NA	57	S39G (AGC→GGC)	sbmC	DNA gyrase inhibitor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/24);  total (33/24)

CAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGT  >  NC_000913/2081005‑2081295                                                                                                                                                |                                                                                                                                                    cAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGGTTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTAcc                                                                                                                                              >  1:898902/1‑151 (MQ=255) cAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATTTAcc                                                                                                                                              >  1:775495/1‑151 (MQ=255) cAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGAAAGCAACCCACTCCTTCGGCACAAAATTTTTGCCATTTAcc                                                                                                                                              >  2:15657/1‑151 (MQ=255)        aCGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCAcatca                                                                                                                                       <  1:846589/151‑1 (MQ=255)            ccTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACatcatc                                                                                                                                     >  2:166842/1‑149 (MQ=255)               cACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTg                                                                                                                                <  1:64093/151‑1 (MQ=255)                   gTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCa                                                                                                                            <  1:392894/151‑1 (MQ=255)                    ttttCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTc                                                                                                                             >  2:138824/1‑149 (MQ=255)                          ggAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCctt                                                                                                                     <  1:240848/151‑1 (MQ=255)                              gCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCctttctt                                                                                                                 <  2:521940/151‑1 (MQ=255)                               cGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCctttcttt                                                                                                                <  2:92602/151‑1 (MQ=255)                                gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTa                                                                                                               <  2:630735/151‑1 (MQ=255)                                gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAACCACTCCTTCGGCACAATATTTTTGCCATCTACCCAAATCATCAAATGCTCAAAGCctttcttt                                                                                                                >  1:496074/1‑150 (MQ=255)                                gTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGGGGTTCATCTGGGTTGTTGTAATAGTCAGCAACCCACTCCTTCGGCACAATATTTTTTTCATCTACCCACATCCTCAACTGGTCAAAATctttctttt                                                                                                               >  2:92822/1‑150 (MQ=255)                                 tAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTa                                                                                                               >  1:31219/1‑150 (MQ=255)                                       aaCCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCt                                                                                                         <  1:222668/150‑1 (MQ=255)                                              cACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCAc                                                                                                 >  1:332534/1‑151 (MQ=255)                                                ccGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATTTGGATTGTCGGAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTTCCCCgg                                                                                               >  2:36843/1‑151 (MQ=255)                                                ccGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAAACCCCTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACgg                                                                                               >  2:550906/1‑151 (MQ=255)                                                    cACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCa                                                                                           <  2:773622/151‑1 (MQ=255)                                                       gacgGTGTCGCAGCGTAATTTTTCGGCGGGGGTTTCATCTGGGTTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTTCCACGGGCCAACg                                                                                        >  2:822123/1‑151 (MQ=255)                                                          ggTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAAc                                                                                         >  1:222438/1‑147 (MQ=255)                                                           gtgtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGAt                                                                                    <  1:813589/151‑1 (MQ=255)                                                             gtCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATgg                                                                                  <  1:634385/151‑1 (MQ=255)                                                                     gTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGGACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAACCTTTCTTTAACGTCTGTTCCCACGGGCCAACGAGATGGGAACCTg                                                                           >  1:538679/1‑150 (MQ=255)                                                                     gTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGc                                                                          >  1:687918/1‑151 (MQ=255)                                                                          ttttCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAAACCGCAACgg                                                                     >  2:5628/1‑151 (MQ=255)                                                                             tCGGCGGGTGTTTCATCTGGATTGTCGGAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACgg                                                                     >  2:585585/1‑148 (MQ=255)                                                                                 cggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                              <  2:297627/151‑1 (MQ=255)                                                                                 cggGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGttt                                                              >  2:203471/1‑151 (MQ=255)                                                                                       tttCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTc                                                        >  1:502551/1‑151 (MQ=255)                                                                                          cATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCt                                                      <  1:102089/150‑1 (MQ=255)                                                                                          cATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTg                                                     <  1:183069/151‑1 (MQ=255)                                                                                            tCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCt                                                   >  1:542440/1‑151 (MQ=255)                                                                                               ggATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTaa                                                <  1:329189/151‑1 (MQ=255)                                                                                                gATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGc                                                    <  1:843003/146‑1 (MQ=255)                                                                                                    gTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCg                                           >  1:24505/1‑151 (MQ=255)                                                                                                    gTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCg                                           <  2:577145/151‑1 (MQ=255)                                                                                                      cGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGt                                          <  1:945082/150‑1 (MQ=255)                                                                                                      cGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGt                                          <  2:26522/150‑1 (MQ=255)                                                                                                      cGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCACACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGt                                          >  1:446527/1‑150 (MQ=255)                                                                                                        tAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGt                                       >  1:750200/1‑151 (MQ=255)                                                                                                          aTAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTc                                     >  1:161923/1‑151 (MQ=255)                                                                                                                aGCAACCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACg                               <  1:649331/151‑1 (MQ=255)                                                                                                                   aaCCCACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTCTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCt                            >  1:713862/1‑151 (MQ=255)                                                                                                                     cccACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                          <  2:308928/151‑1 (MQ=255)                                                                                                                     cccACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTcc                          <  2:711911/151‑1 (MQ=255)                                                                                                                      ccACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                         <  1:187461/151‑1 (MQ=255)                                                                                                                      ccACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCa                         <  2:210894/151‑1 (MQ=255)                                                                                                                       cACTCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCAt                        >  2:592517/1‑151 (MQ=255)                                                                                                                          tCCTTCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTg                      >  1:360209/1‑150 (MQ=255)                                                                                                                             ttCGGCACAATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATAc                  >  2:670442/1‑151 (MQ=255)                                                                                                                                   acaATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGtttt            >  2:872617/1‑151 (MQ=255)                                                                                                                                    caATATTTTTGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGttttt           >  2:358552/1‑151 (MQ=255)                                                                                                                                         tttttGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTtatat       >  2:793940/1‑150 (MQ=255)                                                                                                                                           tttGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATAc     >  2:11753/1‑150 (MQ=255)                                                                                                                                             tGCCATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGt  >  2:594205/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                    CAGAATGACGCCCTCACTGTTTTCGGGAAGCGTAAAGTAACCCGGCACCGTCACGACGGTGTCGCAGCGTAATTTTTCGGCGGGTGTTTCATCTGGATTGTCGTAATAGACAGCAACCCACTCCTTCGGCACAATATTTTTGCTATCTACCCACATCATCAACTGCTCAAAGCCTTTCTTTACCGTCTGTTCCCACGGGCCAACGAGATGGAAACCTGCAACGGTACGTTTCTCTTCCTGCTTAATCTCGTAGTTCATGACGCCTCCATTGATACTGTTTTTATATACAGT  >  NC_000913/2081005‑2081295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑