Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,010	T→C	E106E (GAA→GAG)	wcaN ←	putative regulatory subunit for GalU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,010	0	T	C	100.0%	201.8 / NA	55	E106E (GAA→GAG)	wcaN	putative regulatory subunit for GalU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/26);  total (29/26)

CGTTGAAACGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCG  >  NC_000913/2113862‑2114157                                                                                                                                                     |                                                                                                                                                    cGTTGAAACGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTc                                                                                                                                                   <  1:98345/151‑1 (MQ=255) cGTTGAAACGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTc                                                                                                                                                   >  2:412032/1‑151 (MQ=255)    tGAAACGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGc                                                                                                                                                 <  1:305503/150‑1 (MQ=255)       aaCGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTg                                                                                                                                             >  1:655207/1‑151 (MQ=255)        aCGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTg                                                                                                                                             >  2:144412/1‑150 (MQ=255)        aCGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTg                                                                                                                                             >  2:816749/1‑150 (MQ=255)              aaTCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACg                                                                                                                                      >  2:878606/1‑151 (MQ=255)                    agcagcaAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCAt                                                                                                                                 >  1:858452/2‑150 (MQ=255)                           aGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTc                                                                                                                          >  1:297221/1‑150 (MQ=255)                                  aaCGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCcgg                                                                                                                  >  2:342376/1‑151 (MQ=255)                                        gCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACa                                                                                                            <  2:141991/151‑1 (MQ=255)                                        gCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACa                                                                                                            <  2:306135/151‑1 (MQ=255)                                            gTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATg                                                                                                        <  2:276290/151‑1 (MQ=255)                                              cGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGa                                                                                                      <  1:910464/151‑1 (MQ=255)                                               ggCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGAc                                                                                                     <  1:264427/151‑1 (MQ=255)                                                  gcTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGt                                                                                                  >  2:225900/1‑151 (MQ=255)                                                   cTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGt                                                                                                  >  1:884008/1‑150 (MQ=255)                                                         tcgtcgATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACtt                                                                                              >  2:563281/1‑148 (MQ=255)                                                           gtcgATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGcc                                                                                         >  1:198464/1‑151 (MQ=255)                                                             cgATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGcc                                                                                         >  2:165740/1‑149 (MQ=255)                                                             cgATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGcc                                                                                         >  2:278477/1‑149 (MQ=255)                                                              gATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCcagc                                                                                      >  2:206168/1‑151 (MQ=255)                                                               aTCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCcagc                                                                                      <  1:196107/150‑1 (MQ=255)                                                                              aGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCAcgc                                                                      <  1:304483/151‑1 (MQ=255)                                                                              aGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCAcg                                                                       <  2:828190/150‑1 (MQ=255)                                                                                   cACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCgctgc                                                                 <  2:853664/151‑1 (MQ=255)                                                                                     cGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTc                                                               <  1:223279/151‑1 (MQ=255)                                                                                      gACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTc                                                               >  1:650764/1‑150 (MQ=255)                                                                                      gACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTc                                                               >  2:828704/1‑150 (MQ=255)                                                                                        cAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCa                                                              >  2:628106/1‑149 (MQ=255)                                                                                                ttGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGAtt                                                    >  1:115319/1‑151 (MQ=255)                                                                                                ttGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGAtt                                                    >  1:885470/1‑151 (MQ=255)                                                                                                    cACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTa                                                 <  1:906846/150‑1 (MQ=255)                                                                                                       cAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTc                                             <  1:374241/151‑1 (MQ=255)                                                                                                         aTGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCAt                                           >  1:109263/1‑151 (MQ=255)                                                                                                         aTGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCAt                                           >  1:213758/1‑151 (MQ=255)                                                                                                           ggCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATa                                          <  2:209545/150‑1 (MQ=255)                                                                                                           ggCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATa                                          <  1:643752/150‑1 (MQ=255)                                                                                                            gCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAag                                        <  2:410488/151‑1 (MQ=255)                                                                                                             cAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAaga                                       <  2:881233/151‑1 (MQ=255)                                                                                                             cAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAaga                                       <  1:643540/151‑1 (MQ=255)                                                                                                                gTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGgt                                    <  2:90437/151‑1 (MQ=255)                                                                                                                  cgcgCACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGgtg                                   <  1:266005/150‑1 (MQ=255)                                                                                                                    cgcACACAAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCg                                <  2:683898/151‑1 (MQ=255)                                                                                                                       acacaAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAg                             <  2:516786/151‑1 (MQ=255)                                                                                                                        cacaAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGt                            >  1:728008/1‑151 (MQ=255)                                                                                                                         acaAAATGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTg                           <  2:684475/151‑1 (MQ=255)                                                                                                                              aTGGAGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGtttt                      >  2:582011/1‑151 (MQ=255)                                                                                                                                 aaGTGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCg                    >  1:544946/2‑150 (MQ=255)                                                                                                                                    tGGCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACcg                >  1:661271/1‑151 (MQ=255)                                                                                                                                     ggCCTAAACCTAAAGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACcgc               <  1:409027/151‑1 (MQ=255)                                                                                                                                          aaacctaaaGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCt          >  2:462931/1‑151 (MQ=255)                                                                                                                                              ctaaaGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTgg       >  1:153583/1‑150 (MQ=255)                                                                                                                                               taaaGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGAc     >  2:742343/1‑151 (MQ=255)                                                                                                                                                  aGGCTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGAcgcg  <  2:677835/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGTTGAAACGTGCAATCATGGCAGCAAGGTTGTAACGTAGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCG  >  NC_000913/2113862‑2114157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑